Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_00200 and ydaS overlap by 4 nucleotides ydaS and NIAGMN_00210 are separated by 97 nucleotides NIAGMN_00210 and NIAGMN_00215 are separated by 20 nucleotides NIAGMN_00215 and parE are separated by 1 nucleotides parE and NIAGMN_00225 are separated by 295 nucleotides NIAGMN_00225 and NIAGMN_00230 are separated by 71 nucleotides NIAGMN_00230 and NIAGMN_00235 are separated by 3 nucleotides
NIAGMN_00200: NIAGMN_00200 - Rha family transcriptional regulator, at 25,978 to 26,403
_00200
NIAGMN_00205: ydaS - Putative antitoxin of bacterial toxin-antitoxin system, YdaS/YdaT, at 26,400 to 26,702
ydaS
NIAGMN_00210: NIAGMN_00210 - Cro/Cl family transcriptional regulator, at 26,800 to 27,171
_00210
NIAGMN_00215: NIAGMN_00215 - stability determinant, at 27,192 to 27,383
_00215
NIAGMN_00220: parE - plasmid stabilization protein ParE, at 27,385 to 27,663
parE
NIAGMN_00225: NIAGMN_00225 - hypothetical protein, at 27,959 to 28,315
_00225
NIAGMN_00230: NIAGMN_00230 - Uncharacterized protein YdfC, at 28,387 to 28,605
_00230
NIAGMN_00235: NIAGMN_00235 - hypothetical protein, at 28,609 to 28,773
_00235
Position (kb) 27
28 Strain fitness (log2 ratio) -1
0
1
2 at 26.455 kb on - strand, within ydaS at 26.501 kb on + strand, within ydaS at 26.502 kb on - strand, within ydaS at 26.568 kb on - strand, within ydaS at 26.587 kb on - strand, within ydaS at 26.588 kb on + strand, within ydaS at 26.589 kb on - strand, within ydaS at 26.589 kb on - strand, within ydaS at 26.620 kb on + strand, within ydaS at 26.703 kb on - strand at 27.176 kb on + strand at 27.176 kb on + strand at 27.425 kb on + strand, within parE at 27.432 kb on + strand, within parE at 27.434 kb on + strand, within parE at 27.527 kb on - strand, within parE
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 26,455 - ydaS NIAGMN_00205 0.18 -0.3 26,501 + ydaS NIAGMN_00205 0.33 -0.7 26,502 - ydaS NIAGMN_00205 0.34 +0.2 26,568 - ydaS NIAGMN_00205 0.55 -0.9 26,587 - ydaS NIAGMN_00205 0.62 -0.3 26,588 + ydaS NIAGMN_00205 0.62 -1.5 26,589 - ydaS NIAGMN_00205 0.62 -1.2 26,589 - ydaS NIAGMN_00205 0.62 +0.0 26,620 + ydaS NIAGMN_00205 0.73 +1.1 26,703 - -1.5 27,176 + +2.0 27,176 + -0.4 27,425 + parE NIAGMN_00220 0.14 -0.9 27,432 + parE NIAGMN_00220 0.17 -0.7 27,434 + parE NIAGMN_00220 0.18 -0.2 27,527 - parE NIAGMN_00220 0.51 +1.9
Or see this region's nucleotide sequence