Strain Fitness in Escherichia coli ECRC102 around NIAGMN_00185

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntNIAGMN_00175 and NIAGMN_00180 are separated by 56 nucleotidesNIAGMN_00180 and NIAGMN_00185 are separated by 15 nucleotidesNIAGMN_00185 and dnaC are separated by 126 nucleotidesdnaC and NIAGMN_00195 are separated by 6 nucleotides NIAGMN_00175: NIAGMN_00175 - DUF551 domain-containing protein, at 22,399 to 22,983 _00175 NIAGMN_00180: NIAGMN_00180 - DUF977 domain-containing protein, at 23,040 to 23,435 _00180 NIAGMN_00185: NIAGMN_00185 - DUF1627 domain-containing protein, at 23,451 to 24,119 _00185 NIAGMN_00190: dnaC - DNA replication protein DnaC, at 24,246 to 24,986 dnaC NIAGMN_00195: NIAGMN_00195 - DNA-binding protein, at 24,993 to 25,955 _00195 Position (kb) 23 24 25Strain fitness (log2 ratio) -2 -1 0 1at 22.733 kb on - strand, within NIAGMN_00175at 22.745 kb on - strand, within NIAGMN_00175at 22.972 kb on - strandat 23.029 kb on - strandat 23.790 kb on + strand, within NIAGMN_00185at 23.823 kb on - strand, within NIAGMN_00185at 24.128 kb on - strandat 24.252 kb on - strandat 24.324 kb on - strand, within dnaCat 24.396 kb on - strand, within dnaCat 24.663 kb on - strand, within dnaC

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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22,733 - NIAGMN_00175 0.57 -0.9
22,745 - NIAGMN_00175 0.59 +0.7
22,972 - +0.7
23,029 - +0.2
23,790 + NIAGMN_00185 0.51 +0.2
23,823 - NIAGMN_00185 0.56 +0.3
24,128 - +1.2
24,252 - +0.3
24,324 - dnaC NIAGMN_00190 0.11 -0.3
24,396 - dnaC NIAGMN_00190 0.20 -1.8
24,663 - dnaC NIAGMN_00190 0.56 +1.6

Or see this region's nucleotide sequence