Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mhpF and mhpD overlap by 4 nucleotides mhpD and mhpC are separated by 9 nucleotides mhpC and mhpB are separated by 17 nucleotides mhpB and mhpA are separated by 1 nucleotides
MCAODC_27995: mhpF - acetaldehyde dehydrogenase, at 2,347,998 to 2,348,948
mhpF
MCAODC_28000: mhpD - 2-keto-4-pentenoate hydratase, at 2,348,945 to 2,349,754
mhpD
MCAODC_28005: mhpC - 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase, at 2,349,764 to 2,350,630
mhpC
MCAODC_28010: mhpB - 3-carboxyethylcatechol 2,3-dioxygenase, at 2,350,648 to 2,351,592
mhpB
MCAODC_28015: mhpA - bifunctional 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase, at 2,351,594 to 2,353,258
mhpA
Position (kb)
2349
2350
2351 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2348.801 kb on + strand, within mhpF at 2348.802 kb on - strand, within mhpF at 2348.802 kb on - strand, within mhpF at 2348.936 kb on + strand at 2348.937 kb on - strand at 2348.942 kb on + strand at 2348.942 kb on + strand at 2348.943 kb on - strand at 2348.999 kb on + strand at 2348.999 kb on + strand at 2349.000 kb on - strand at 2349.010 kb on + strand at 2349.056 kb on + strand, within mhpD at 2349.056 kb on + strand, within mhpD at 2349.056 kb on + strand, within mhpD at 2349.057 kb on - strand, within mhpD at 2349.228 kb on - strand, within mhpD at 2349.264 kb on + strand, within mhpD at 2349.264 kb on + strand, within mhpD at 2349.264 kb on + strand, within mhpD at 2349.264 kb on + strand, within mhpD at 2349.264 kb on + strand, within mhpD at 2349.264 kb on + strand, within mhpD at 2349.264 kb on + strand, within mhpD at 2349.264 kb on + strand, within mhpD at 2349.264 kb on + strand, within mhpD at 2349.264 kb on + strand, within mhpD at 2349.265 kb on - strand, within mhpD at 2349.265 kb on - strand, within mhpD at 2349.265 kb on - strand, within mhpD at 2349.265 kb on - strand, within mhpD at 2349.283 kb on + strand, within mhpD at 2349.374 kb on - strand, within mhpD at 2349.375 kb on + strand, within mhpD at 2349.375 kb on + strand, within mhpD at 2349.375 kb on + strand, within mhpD at 2349.376 kb on - strand, within mhpD at 2349.376 kb on - strand, within mhpD at 2349.394 kb on + strand, within mhpD at 2349.509 kb on + strand, within mhpD at 2349.509 kb on + strand, within mhpD at 2349.509 kb on + strand, within mhpD at 2349.510 kb on - strand, within mhpD at 2349.510 kb on - strand, within mhpD at 2349.578 kb on - strand, within mhpD at 2349.613 kb on - strand, within mhpD at 2349.623 kb on + strand, within mhpD at 2349.623 kb on + strand, within mhpD at 2349.623 kb on + strand, within mhpD at 2349.623 kb on + strand, within mhpD at 2349.623 kb on + strand, within mhpD at 2349.624 kb on - strand, within mhpD at 2349.624 kb on - strand, within mhpD at 2349.624 kb on - strand, within mhpD at 2349.639 kb on + strand, within mhpD at 2349.742 kb on + strand at 2349.743 kb on - strand at 2349.765 kb on + strand at 2349.850 kb on + strand at 2349.850 kb on + strand at 2349.850 kb on + strand at 2349.851 kb on - strand, within mhpC at 2349.851 kb on - strand, within mhpC at 2350.010 kb on - strand, within mhpC at 2350.045 kb on + strand, within mhpC at 2350.045 kb on + strand, within mhpC at 2350.045 kb on + strand, within mhpC at 2350.046 kb on - strand, within mhpC at 2350.049 kb on - strand, within mhpC at 2350.090 kb on + strand, within mhpC at 2350.146 kb on + strand, within mhpC at 2350.165 kb on + strand, within mhpC at 2350.165 kb on + strand, within mhpC at 2350.166 kb on - strand, within mhpC at 2350.168 kb on + strand, within mhpC at 2350.169 kb on - strand, within mhpC at 2350.169 kb on - strand, within mhpC at 2350.189 kb on + strand, within mhpC at 2350.276 kb on + strand, within mhpC at 2350.277 kb on - strand, within mhpC at 2350.277 kb on - strand, within mhpC at 2350.277 kb on - strand, within mhpC at 2350.379 kb on - strand, within mhpC at 2350.460 kb on - strand, within mhpC at 2350.581 kb on - strand at 2350.623 kb on + strand at 2350.646 kb on - strand at 2350.669 kb on + strand at 2350.670 kb on - strand at 2350.713 kb on - strand at 2350.726 kb on - strand at 2350.843 kb on + strand, within mhpB at 2350.843 kb on + strand, within mhpB at 2350.844 kb on - strand, within mhpB at 2350.844 kb on - strand, within mhpB at 2351.077 kb on + strand, within mhpB at 2351.206 kb on - strand, within mhpB at 2351.262 kb on + strand, within mhpB at 2351.262 kb on + strand, within mhpB at 2351.262 kb on + strand, within mhpB at 2351.262 kb on + strand, within mhpB at 2351.262 kb on + strand, within mhpB at 2351.262 kb on + strand, within mhpB at 2351.263 kb on - strand, within mhpB at 2351.263 kb on - strand, within mhpB at 2351.263 kb on - strand, within mhpB at 2351.263 kb on - strand, within mhpB at 2351.263 kb on - strand, within mhpB at 2351.417 kb on + strand, within mhpB at 2351.433 kb on - strand, within mhpB at 2351.433 kb on - strand, within mhpB at 2351.433 kb on - strand, within mhpB at 2351.433 kb on - strand, within mhpB at 2351.482 kb on - strand, within mhpB at 2351.482 kb on - strand, within mhpB at 2351.547 kb on - strand at 2351.582 kb on + strand at 2351.582 kb on + strand at 2351.593 kb on - strand at 2351.593 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 2,348,801 + mhpF MCAODC_27995 0.84 -1.4 2,348,802 - mhpF MCAODC_27995 0.85 +1.3 2,348,802 - mhpF MCAODC_27995 0.85 +0.5 2,348,936 + +0.6 2,348,937 - -1.2 2,348,942 + +1.4 2,348,942 + +0.5 2,348,943 - -0.7 2,348,999 + -1.1 2,348,999 + -0.8 2,349,000 - -0.5 2,349,010 + +0.5 2,349,056 + mhpD MCAODC_28000 0.14 -0.2 2,349,056 + mhpD MCAODC_28000 0.14 -0.6 2,349,056 + mhpD MCAODC_28000 0.14 -2.0 2,349,057 - mhpD MCAODC_28000 0.14 -0.2 2,349,228 - mhpD MCAODC_28000 0.35 -2.0 2,349,264 + mhpD MCAODC_28000 0.39 +0.6 2,349,264 + mhpD MCAODC_28000 0.39 +1.8 2,349,264 + mhpD MCAODC_28000 0.39 -0.3 2,349,264 + mhpD MCAODC_28000 0.39 -2.2 2,349,264 + mhpD MCAODC_28000 0.39 -0.6 2,349,264 + mhpD MCAODC_28000 0.39 +1.9 2,349,264 + mhpD MCAODC_28000 0.39 +0.1 2,349,264 + mhpD MCAODC_28000 0.39 +1.2 2,349,264 + mhpD MCAODC_28000 0.39 +0.4 2,349,264 + mhpD MCAODC_28000 0.39 -0.7 2,349,265 - mhpD MCAODC_28000 0.40 +0.3 2,349,265 - mhpD MCAODC_28000 0.40 +0.3 2,349,265 - mhpD MCAODC_28000 0.40 +1.1 2,349,265 - mhpD MCAODC_28000 0.40 -0.3 2,349,283 + mhpD MCAODC_28000 0.42 +0.7 2,349,374 - mhpD MCAODC_28000 0.53 +1.1 2,349,375 + mhpD MCAODC_28000 0.53 +1.0 2,349,375 + mhpD MCAODC_28000 0.53 -0.4 2,349,375 + mhpD MCAODC_28000 0.53 +1.4 2,349,376 - mhpD MCAODC_28000 0.53 -1.7 2,349,376 - mhpD MCAODC_28000 0.53 +1.2 2,349,394 + mhpD MCAODC_28000 0.55 +0.1 2,349,509 + mhpD MCAODC_28000 0.70 +0.2 2,349,509 + mhpD MCAODC_28000 0.70 +1.3 2,349,509 + mhpD MCAODC_28000 0.70 +0.5 2,349,510 - mhpD MCAODC_28000 0.70 -4.6 2,349,510 - mhpD MCAODC_28000 0.70 -1.0 2,349,578 - mhpD MCAODC_28000 0.78 -0.3 2,349,613 - mhpD MCAODC_28000 0.82 -3.1 2,349,623 + mhpD MCAODC_28000 0.84 -2.6 2,349,623 + mhpD MCAODC_28000 0.84 +0.3 2,349,623 + mhpD MCAODC_28000 0.84 +0.1 2,349,623 + mhpD MCAODC_28000 0.84 -0.8 2,349,623 + mhpD MCAODC_28000 0.84 -0.8 2,349,624 - mhpD MCAODC_28000 0.84 -3.0 2,349,624 - mhpD MCAODC_28000 0.84 +0.4 2,349,624 - mhpD MCAODC_28000 0.84 +1.2 2,349,639 + mhpD MCAODC_28000 0.86 -0.8 2,349,742 + +0.6 2,349,743 - -0.6 2,349,765 + +1.1 2,349,850 + -0.6 2,349,850 + +1.6 2,349,850 + +0.3 2,349,851 - mhpC MCAODC_28005 0.10 +0.4 2,349,851 - mhpC MCAODC_28005 0.10 +1.6 2,350,010 - mhpC MCAODC_28005 0.28 -0.3 2,350,045 + mhpC MCAODC_28005 0.32 -0.8 2,350,045 + mhpC MCAODC_28005 0.32 -2.1 2,350,045 + mhpC MCAODC_28005 0.32 -1.0 2,350,046 - mhpC MCAODC_28005 0.33 +0.4 2,350,049 - mhpC MCAODC_28005 0.33 -0.2 2,350,090 + mhpC MCAODC_28005 0.38 +1.5 2,350,146 + mhpC MCAODC_28005 0.44 +1.0 2,350,165 + mhpC MCAODC_28005 0.46 -0.6 2,350,165 + mhpC MCAODC_28005 0.46 +0.3 2,350,166 - mhpC MCAODC_28005 0.46 +0.9 2,350,168 + mhpC MCAODC_28005 0.47 +4.0 2,350,169 - mhpC MCAODC_28005 0.47 -2.3 2,350,169 - mhpC MCAODC_28005 0.47 +0.1 2,350,189 + mhpC MCAODC_28005 0.49 -1.8 2,350,276 + mhpC MCAODC_28005 0.59 +0.7 2,350,277 - mhpC MCAODC_28005 0.59 -2.1 2,350,277 - mhpC MCAODC_28005 0.59 -0.8 2,350,277 - mhpC MCAODC_28005 0.59 +0.6 2,350,379 - mhpC MCAODC_28005 0.71 +0.0 2,350,460 - mhpC MCAODC_28005 0.80 -1.5 2,350,581 - -1.5 2,350,623 + -0.0 2,350,646 - +0.5 2,350,669 + -0.5 2,350,670 - +0.1 2,350,713 - -0.6 2,350,726 - -0.6 2,350,843 + mhpB MCAODC_28010 0.21 +0.1 2,350,843 + mhpB MCAODC_28010 0.21 -0.7 2,350,844 - mhpB MCAODC_28010 0.21 +0.4 2,350,844 - mhpB MCAODC_28010 0.21 -0.1 2,351,077 + mhpB MCAODC_28010 0.45 +0.7 2,351,206 - mhpB MCAODC_28010 0.59 -1.4 2,351,262 + mhpB MCAODC_28010 0.65 +0.2 2,351,262 + mhpB MCAODC_28010 0.65 -0.9 2,351,262 + mhpB MCAODC_28010 0.65 +0.0 2,351,262 + mhpB MCAODC_28010 0.65 -3.0 2,351,262 + mhpB MCAODC_28010 0.65 +0.3 2,351,262 + mhpB MCAODC_28010 0.65 +0.9 2,351,263 - mhpB MCAODC_28010 0.65 +1.2 2,351,263 - mhpB MCAODC_28010 0.65 -1.0 2,351,263 - mhpB MCAODC_28010 0.65 +0.4 2,351,263 - mhpB MCAODC_28010 0.65 +0.3 2,351,263 - mhpB MCAODC_28010 0.65 +0.3 2,351,417 + mhpB MCAODC_28010 0.81 +0.2 2,351,433 - mhpB MCAODC_28010 0.83 +0.2 2,351,433 - mhpB MCAODC_28010 0.83 +0.4 2,351,433 - mhpB MCAODC_28010 0.83 +0.3 2,351,433 - mhpB MCAODC_28010 0.83 +0.3 2,351,482 - mhpB MCAODC_28010 0.88 +2.4 2,351,482 - mhpB MCAODC_28010 0.88 +0.2 2,351,547 - -0.3 2,351,582 + -1.1 2,351,582 + -0.2 2,351,593 - -0.0 2,351,593 - -1.9
Or see this region's nucleotide sequence