Strain Fitness in Escherichia coli ECRC101 around MCAODC_27675

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthiL and nusB are separated by 77 nucleotidesnusB and ribE are separated by 19 nucleotidesribE and ribD are separated by 88 nucleotides MCAODC_27665: thiL - thiamine-phosphate kinase, at 2,279,616 to 2,280,593 thiL MCAODC_27670: nusB - transcription antitermination factor NusB, at 2,280,671 to 2,281,090 nusB MCAODC_27675: ribE - 6,7-dimethyl-8-ribityllumazine synthase, at 2,281,110 to 2,281,580 ribE MCAODC_27680: ribD - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD, at 2,281,669 to 2,282,772 ribD Position (kb) 2281 2282Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2280.823 kb on - strand, within nusBat 2281.627 kb on - strandat 2281.635 kb on - strandat 2281.635 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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2,280,823 - nusB MCAODC_27670 0.36 -2.9
2,281,627 - +2.0
2,281,635 - -0.5
2,281,635 - -0.0

Or see this region's nucleotide sequence