Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hokE and hokE are separated by 350 nucleotides hokE and ybdK are separated by 438 nucleotides ybdK and ybdJ are separated by 65 nucleotides ybdJ and ybdF are separated by 64 nucleotides ybdF and nfsB are separated by 93 nucleotides
MCAODC_26850: hokE - type I toxin-antitoxin system toxin HokE, at 2,089,800 to 2,089,952
hokE
MCAODC_26860: hokE - type I toxin-antitoxin system toxin HokE, at 2,090,303 to 2,090,455
hokE
MCAODC_26870: ybdK - YbdK family carboxylate-amine ligase, at 2,090,894 to 2,092,012
ybdK
MCAODC_26875: ybdJ - Uncharacterized protein YbdJ, at 2,092,078 to 2,092,326
ybdJ
MCAODC_26880: ybdF - MmcQ/YjbR family DNA-binding protein, at 2,092,391 to 2,092,759
ybdF
MCAODC_26885: nfsB - oxygen-insensitive NAD(P)H nitroreductase, at 2,092,853 to 2,093,506
nfsB
Position (kb)
2090
2091
2092
2093 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2089.921 kb on - strand, within hokE at 2089.921 kb on - strand, within hokE at 2089.956 kb on + strand at 2089.958 kb on + strand at 2089.958 kb on + strand at 2089.983 kb on + strand at 2090.028 kb on + strand at 2090.028 kb on + strand at 2090.034 kb on + strand at 2090.035 kb on - strand at 2090.035 kb on - strand at 2090.035 kb on - strand at 2090.071 kb on - strand at 2090.105 kb on + strand at 2090.120 kb on + strand at 2090.192 kb on + strand at 2090.238 kb on - strand at 2090.238 kb on - strand at 2090.320 kb on - strand, within hokE at 2090.320 kb on - strand, within hokE at 2090.347 kb on + strand, within hokE at 2090.347 kb on + strand, within hokE at 2090.347 kb on + strand, within hokE at 2090.347 kb on + strand, within hokE at 2090.348 kb on - strand, within hokE at 2090.348 kb on - strand, within hokE at 2090.348 kb on - strand, within hokE at 2090.396 kb on - strand, within hokE at 2090.396 kb on - strand, within hokE at 2090.424 kb on - strand, within hokE at 2090.424 kb on - strand, within hokE at 2090.424 kb on - strand, within hokE at 2090.459 kb on + strand at 2090.461 kb on + strand at 2090.486 kb on + strand at 2090.486 kb on + strand at 2090.486 kb on + strand at 2090.530 kb on + strand at 2090.536 kb on + strand at 2090.536 kb on + strand at 2090.536 kb on + strand at 2090.537 kb on - strand at 2090.681 kb on + strand at 2090.681 kb on + strand at 2090.681 kb on + strand at 2090.682 kb on - strand at 2090.682 kb on - strand at 2090.859 kb on - strand at 2090.861 kb on - strand at 2090.868 kb on - strand at 2090.883 kb on - strand at 2090.893 kb on + strand at 2090.893 kb on + strand at 2090.929 kb on + strand at 2090.930 kb on - strand at 2090.982 kb on + strand at 2090.982 kb on + strand at 2090.982 kb on + strand at 2090.983 kb on - strand at 2091.017 kb on - strand, within ybdK at 2091.043 kb on - strand, within ybdK at 2091.043 kb on - strand, within ybdK at 2091.064 kb on + strand, within ybdK at 2091.065 kb on - strand, within ybdK at 2091.065 kb on - strand, within ybdK at 2091.097 kb on + strand, within ybdK at 2091.098 kb on - strand, within ybdK at 2091.222 kb on + strand, within ybdK at 2091.222 kb on + strand, within ybdK at 2091.222 kb on + strand, within ybdK at 2091.266 kb on + strand, within ybdK at 2091.338 kb on + strand, within ybdK at 2091.338 kb on + strand, within ybdK at 2091.339 kb on - strand, within ybdK at 2091.339 kb on - strand, within ybdK at 2091.401 kb on + strand, within ybdK at 2091.401 kb on + strand, within ybdK at 2091.402 kb on - strand, within ybdK at 2091.402 kb on - strand, within ybdK at 2091.402 kb on - strand, within ybdK at 2091.403 kb on + strand, within ybdK at 2091.403 kb on + strand, within ybdK at 2091.403 kb on + strand, within ybdK at 2091.403 kb on + strand, within ybdK at 2091.403 kb on + strand, within ybdK at 2091.403 kb on + strand, within ybdK at 2091.403 kb on + strand, within ybdK at 2091.404 kb on - strand, within ybdK at 2091.404 kb on - strand, within ybdK at 2091.404 kb on - strand, within ybdK at 2091.404 kb on - strand, within ybdK at 2091.404 kb on - strand, within ybdK at 2091.404 kb on - strand, within ybdK at 2091.404 kb on - strand, within ybdK at 2091.404 kb on - strand, within ybdK at 2091.404 kb on - strand, within ybdK at 2091.649 kb on + strand, within ybdK at 2091.650 kb on - strand, within ybdK at 2091.650 kb on - strand, within ybdK at 2091.709 kb on + strand, within ybdK at 2091.710 kb on - strand, within ybdK at 2091.731 kb on + strand, within ybdK at 2091.731 kb on + strand, within ybdK at 2091.732 kb on - strand, within ybdK at 2091.791 kb on + strand, within ybdK at 2091.791 kb on + strand, within ybdK at 2091.862 kb on + strand, within ybdK at 2091.863 kb on - strand, within ybdK at 2092.011 kb on - strand at 2092.028 kb on - strand at 2092.037 kb on + strand at 2092.068 kb on + strand at 2092.069 kb on - strand at 2092.078 kb on - strand at 2092.173 kb on + strand, within ybdJ at 2092.219 kb on - strand, within ybdJ at 2092.219 kb on - strand, within ybdJ at 2092.236 kb on - strand, within ybdJ at 2092.276 kb on + strand, within ybdJ at 2092.277 kb on - strand, within ybdJ at 2092.293 kb on + strand, within ybdJ at 2092.324 kb on + strand at 2092.325 kb on - strand at 2092.355 kb on + strand at 2092.356 kb on - strand at 2092.356 kb on - strand at 2092.356 kb on - strand at 2092.440 kb on + strand, within ybdF at 2092.528 kb on - strand, within ybdF at 2092.603 kb on + strand, within ybdF at 2092.604 kb on - strand, within ybdF at 2092.624 kb on + strand, within ybdF at 2092.624 kb on + strand, within ybdF at 2092.625 kb on - strand, within ybdF at 2092.647 kb on - strand, within ybdF at 2092.674 kb on + strand, within ybdF at 2092.675 kb on - strand, within ybdF at 2092.727 kb on - strand at 2092.770 kb on + strand at 2092.770 kb on + strand at 2092.771 kb on - strand at 2092.812 kb on + strand at 2092.813 kb on - strand at 2092.813 kb on - strand at 2092.859 kb on - strand at 2092.958 kb on + strand, within nfsB at 2092.959 kb on - strand, within nfsB
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 2,089,921 - hokE MCAODC_26850 0.79 -0.8 2,089,921 - hokE MCAODC_26850 0.79 -0.3 2,089,956 + +0.3 2,089,958 + +1.5 2,089,958 + -1.3 2,089,983 + -0.8 2,090,028 + -0.2 2,090,028 + -0.5 2,090,034 + -1.6 2,090,035 - -2.5 2,090,035 - -0.6 2,090,035 - -0.9 2,090,071 - -2.6 2,090,105 + +0.3 2,090,120 + -1.5 2,090,192 + +0.9 2,090,238 - -2.6 2,090,238 - -0.4 2,090,320 - hokE MCAODC_26860 0.11 +0.6 2,090,320 - hokE MCAODC_26860 0.11 +1.0 2,090,347 + hokE MCAODC_26860 0.29 +0.6 2,090,347 + hokE MCAODC_26860 0.29 +0.1 2,090,347 + hokE MCAODC_26860 0.29 -0.4 2,090,347 + hokE MCAODC_26860 0.29 +0.2 2,090,348 - hokE MCAODC_26860 0.29 -1.9 2,090,348 - hokE MCAODC_26860 0.29 +0.4 2,090,348 - hokE MCAODC_26860 0.29 -1.0 2,090,396 - hokE MCAODC_26860 0.61 -2.6 2,090,396 - hokE MCAODC_26860 0.61 +2.0 2,090,424 - hokE MCAODC_26860 0.79 -0.3 2,090,424 - hokE MCAODC_26860 0.79 +1.0 2,090,424 - hokE MCAODC_26860 0.79 -0.3 2,090,459 + +1.3 2,090,461 + -0.8 2,090,486 + +0.4 2,090,486 + -0.3 2,090,486 + +0.6 2,090,530 + -0.8 2,090,536 + +1.0 2,090,536 + +0.5 2,090,536 + +0.0 2,090,537 - -0.1 2,090,681 + -0.5 2,090,681 + +1.8 2,090,681 + -0.4 2,090,682 - +1.6 2,090,682 - +0.3 2,090,859 - -0.2 2,090,861 - +0.4 2,090,868 - -0.1 2,090,883 - +1.0 2,090,893 + -0.2 2,090,893 + +0.7 2,090,929 + -1.8 2,090,930 - +0.4 2,090,982 + +0.2 2,090,982 + -0.6 2,090,982 + +1.7 2,090,983 - +0.1 2,091,017 - ybdK MCAODC_26870 0.11 +1.6 2,091,043 - ybdK MCAODC_26870 0.13 +0.8 2,091,043 - ybdK MCAODC_26870 0.13 -0.3 2,091,064 + ybdK MCAODC_26870 0.15 +0.2 2,091,065 - ybdK MCAODC_26870 0.15 -0.1 2,091,065 - ybdK MCAODC_26870 0.15 +0.8 2,091,097 + ybdK MCAODC_26870 0.18 +0.1 2,091,098 - ybdK MCAODC_26870 0.18 +0.3 2,091,222 + ybdK MCAODC_26870 0.29 +0.4 2,091,222 + ybdK MCAODC_26870 0.29 +0.1 2,091,222 + ybdK MCAODC_26870 0.29 +1.6 2,091,266 + ybdK MCAODC_26870 0.33 +0.2 2,091,338 + ybdK MCAODC_26870 0.40 +0.2 2,091,338 + ybdK MCAODC_26870 0.40 +1.0 2,091,339 - ybdK MCAODC_26870 0.40 -0.0 2,091,339 - ybdK MCAODC_26870 0.40 +1.2 2,091,401 + ybdK MCAODC_26870 0.45 -1.3 2,091,401 + ybdK MCAODC_26870 0.45 +0.4 2,091,402 - ybdK MCAODC_26870 0.45 +0.2 2,091,402 - ybdK MCAODC_26870 0.45 -0.1 2,091,402 - ybdK MCAODC_26870 0.45 +0.2 2,091,403 + ybdK MCAODC_26870 0.45 +1.0 2,091,403 + ybdK MCAODC_26870 0.45 -0.3 2,091,403 + ybdK MCAODC_26870 0.45 -0.7 2,091,403 + ybdK MCAODC_26870 0.45 +0.3 2,091,403 + ybdK MCAODC_26870 0.45 -0.2 2,091,403 + ybdK MCAODC_26870 0.45 -1.0 2,091,403 + ybdK MCAODC_26870 0.45 -3.0 2,091,404 - ybdK MCAODC_26870 0.46 +1.4 2,091,404 - ybdK MCAODC_26870 0.46 +0.0 2,091,404 - ybdK MCAODC_26870 0.46 -2.0 2,091,404 - ybdK MCAODC_26870 0.46 +0.4 2,091,404 - ybdK MCAODC_26870 0.46 +0.9 2,091,404 - ybdK MCAODC_26870 0.46 -0.3 2,091,404 - ybdK MCAODC_26870 0.46 -0.7 2,091,404 - ybdK MCAODC_26870 0.46 -3.6 2,091,404 - ybdK MCAODC_26870 0.46 -1.5 2,091,649 + ybdK MCAODC_26870 0.67 -0.0 2,091,650 - ybdK MCAODC_26870 0.68 -0.0 2,091,650 - ybdK MCAODC_26870 0.68 -0.0 2,091,709 + ybdK MCAODC_26870 0.73 +1.2 2,091,710 - ybdK MCAODC_26870 0.73 +0.2 2,091,731 + ybdK MCAODC_26870 0.75 -1.0 2,091,731 + ybdK MCAODC_26870 0.75 +2.0 2,091,732 - ybdK MCAODC_26870 0.75 -1.5 2,091,791 + ybdK MCAODC_26870 0.80 +1.7 2,091,791 + ybdK MCAODC_26870 0.80 +0.3 2,091,862 + ybdK MCAODC_26870 0.87 +0.5 2,091,863 - ybdK MCAODC_26870 0.87 -2.0 2,092,011 - +2.2 2,092,028 - +0.6 2,092,037 + -3.4 2,092,068 + -0.3 2,092,069 - +0.0 2,092,078 - -1.0 2,092,173 + ybdJ MCAODC_26875 0.38 -0.8 2,092,219 - ybdJ MCAODC_26875 0.57 +0.2 2,092,219 - ybdJ MCAODC_26875 0.57 -1.6 2,092,236 - ybdJ MCAODC_26875 0.63 +0.3 2,092,276 + ybdJ MCAODC_26875 0.80 -3.1 2,092,277 - ybdJ MCAODC_26875 0.80 -0.8 2,092,293 + ybdJ MCAODC_26875 0.86 -0.8 2,092,324 + +0.5 2,092,325 - -1.8 2,092,355 + -0.7 2,092,356 - -0.7 2,092,356 - -0.1 2,092,356 - -0.1 2,092,440 + ybdF MCAODC_26880 0.13 +0.2 2,092,528 - ybdF MCAODC_26880 0.37 +0.2 2,092,603 + ybdF MCAODC_26880 0.57 +0.0 2,092,604 - ybdF MCAODC_26880 0.58 +1.0 2,092,624 + ybdF MCAODC_26880 0.63 -0.9 2,092,624 + ybdF MCAODC_26880 0.63 +0.8 2,092,625 - ybdF MCAODC_26880 0.63 -0.6 2,092,647 - ybdF MCAODC_26880 0.69 -1.7 2,092,674 + ybdF MCAODC_26880 0.77 +0.3 2,092,675 - ybdF MCAODC_26880 0.77 +1.9 2,092,727 - +1.0 2,092,770 + -2.7 2,092,770 + -1.8 2,092,771 - +0.3 2,092,812 + -1.2 2,092,813 - +0.3 2,092,813 - +0.2 2,092,859 - +0.9 2,092,958 + nfsB MCAODC_26885 0.16 +3.1 2,092,959 - nfsB MCAODC_26885 0.16 -0.1
Or see this region's nucleotide sequence