Strain Fitness in Escherichia coli ECRC101 around MCAODC_26405

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntasnB and Met_trna are separated by 379 nucleotidesMet_trna and Leu_trna are separated by 10 nucleotidesLeu_trna and Gln_trna are separated by 23 nucleotidesGln_trna and Gln_trna are separated by 34 nucleotidesGln_trna and Met_trna are separated by 15 nucleotidesMet_trna and Gln_trna are separated by 48 nucleotidesGln_trna and Gln_trna are separated by 37 nucleotidesGln_trna and ubiF are separated by 153 nucleotides MCAODC_26395: asnB - asparagine synthase B, at 2,001,453 to 2,003,117 asnB MCAODC_26400: Met_trna - tRNA-Met(cat), at 2,003,497 to 2,003,573 _trna MCAODC_26405: Leu_trna - tRNA-Leu(tag), at 2,003,584 to 2,003,668 _trna MCAODC_26410: Gln_trna - tRNA-Gln(ttg), at 2,003,692 to 2,003,766 _trna MCAODC_26415: Gln_trna - tRNA-Gln(ttg), at 2,003,801 to 2,003,875 _trna MCAODC_26420: Met_trna - tRNA-Met(cat), at 2,003,891 to 2,003,967 _trna MCAODC_26425: Gln_trna - tRNA-Gln(ctg), at 2,004,016 to 2,004,090 _trna MCAODC_26430: Gln_trna - tRNA-Gln(ctg), at 2,004,128 to 2,004,202 _trna MCAODC_26435: ubiF - 3-demethoxyubiquinol 3-hydroxylase, at 2,004,356 to 2,005,531 ubiF Position (kb) 2003 2004Strain fitness (log2 ratio) -2 -1 0 1 2at 2002.598 kb on + strand, within asnBat 2002.598 kb on + strand, within asnBat 2002.598 kb on + strand, within asnBat 2002.599 kb on - strand, within asnBat 2002.599 kb on - strand, within asnBat 2002.599 kb on - strand, within asnBat 2002.599 kb on - strand, within asnBat 2002.599 kb on - strand, within asnBat 2002.602 kb on + strand, within asnBat 2002.602 kb on + strand, within asnBat 2002.602 kb on + strand, within asnBat 2002.602 kb on + strand, within asnBat 2002.602 kb on + strand, within asnBat 2002.602 kb on + strand, within asnBat 2002.602 kb on + strand, within asnBat 2002.602 kb on + strand, within asnBat 2002.602 kb on + strand, within asnBat 2002.602 kb on + strand, within asnBat 2002.602 kb on + strand, within asnBat 2002.602 kb on + strand, within asnBat 2002.602 kb on + strand, within asnBat 2002.603 kb on - strand, within asnBat 2002.603 kb on - strand, within asnBat 2002.603 kb on - strand, within asnBat 2002.770 kb on + strand, within asnBat 2002.770 kb on + strand, within asnBat 2002.771 kb on - strand, within asnBat 2002.771 kb on - strand, within asnBat 2002.771 kb on - strand, within asnBat 2002.771 kb on - strand, within asnBat 2002.828 kb on - strand, within asnBat 2002.911 kb on + strand, within asnBat 2002.938 kb on + strand, within asnBat 2002.938 kb on + strand, within asnBat 2002.939 kb on - strand, within asnBat 2002.967 kb on - strandat 2003.110 kb on - strandat 2003.111 kb on + strandat 2003.111 kb on + strandat 2003.112 kb on - strandat 2003.112 kb on - strandat 2003.112 kb on - strandat 2003.112 kb on - strandat 2003.112 kb on - strandat 2003.112 kb on - strandat 2003.116 kb on - strandat 2003.261 kb on + strandat 2003.261 kb on + strandat 2003.262 kb on - strandat 2003.262 kb on - strandat 2003.281 kb on + strandat 2003.303 kb on - strandat 2003.303 kb on - strandat 2003.343 kb on + strandat 2003.350 kb on + strandat 2003.362 kb on - strandat 2003.385 kb on + strandat 2003.385 kb on + strandat 2003.959 kb on + strand, within Met_trnaat 2004.107 kb on + strandat 2004.107 kb on + strandat 2004.107 kb on + strandat 2004.107 kb on + strandat 2004.114 kb on + strandat 2004.114 kb on + strandat 2004.114 kb on + strandat 2004.114 kb on + strandat 2004.114 kb on + strandat 2004.114 kb on + strandat 2004.323 kb on + strandat 2004.358 kb on - strandat 2004.358 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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2,002,598 + asnB MCAODC_26395 0.69 +0.6
2,002,598 + asnB MCAODC_26395 0.69 -0.1
2,002,598 + asnB MCAODC_26395 0.69 +0.8
2,002,599 - asnB MCAODC_26395 0.69 +0.4
2,002,599 - asnB MCAODC_26395 0.69 -0.9
2,002,599 - asnB MCAODC_26395 0.69 +1.4
2,002,599 - asnB MCAODC_26395 0.69 +1.7
2,002,599 - asnB MCAODC_26395 0.69 -1.7
2,002,602 + asnB MCAODC_26395 0.69 +0.6
2,002,602 + asnB MCAODC_26395 0.69 +1.2
2,002,602 + asnB MCAODC_26395 0.69 +0.8
2,002,602 + asnB MCAODC_26395 0.69 -0.9
2,002,602 + asnB MCAODC_26395 0.69 -0.2
2,002,602 + asnB MCAODC_26395 0.69 +0.6
2,002,602 + asnB MCAODC_26395 0.69 -0.2
2,002,602 + asnB MCAODC_26395 0.69 +0.4
2,002,602 + asnB MCAODC_26395 0.69 -0.0
2,002,602 + asnB MCAODC_26395 0.69 -0.3
2,002,602 + asnB MCAODC_26395 0.69 -2.7
2,002,602 + asnB MCAODC_26395 0.69 +2.0
2,002,602 + asnB MCAODC_26395 0.69 +1.9
2,002,603 - asnB MCAODC_26395 0.69 +0.3
2,002,603 - asnB MCAODC_26395 0.69 +0.9
2,002,603 - asnB MCAODC_26395 0.69 +1.5
2,002,770 + asnB MCAODC_26395 0.79 -0.6
2,002,770 + asnB MCAODC_26395 0.79 -0.5
2,002,771 - asnB MCAODC_26395 0.79 +0.5
2,002,771 - asnB MCAODC_26395 0.79 +0.7
2,002,771 - asnB MCAODC_26395 0.79 +0.6
2,002,771 - asnB MCAODC_26395 0.79 -0.3
2,002,828 - asnB MCAODC_26395 0.83 +1.3
2,002,911 + asnB MCAODC_26395 0.88 -0.2
2,002,938 + asnB MCAODC_26395 0.89 -2.5
2,002,938 + asnB MCAODC_26395 0.89 +2.0
2,002,939 - asnB MCAODC_26395 0.89 +1.0
2,002,967 - +2.1
2,003,110 - +0.3
2,003,111 + +0.8
2,003,111 + -1.8
2,003,112 - -0.2
2,003,112 - +0.1
2,003,112 - +0.8
2,003,112 - -1.6
2,003,112 - +1.3
2,003,112 - +1.5
2,003,116 - +0.9
2,003,261 + +0.1
2,003,261 + +2.0
2,003,262 - -1.9
2,003,262 - -1.3
2,003,281 + -0.3
2,003,303 - -1.2
2,003,303 - +1.5
2,003,343 + +0.6
2,003,350 + -0.8
2,003,362 - +2.6
2,003,385 + -0.6
2,003,385 + -1.9
2,003,959 + Met_trna MCAODC_26420 0.88 +0.3
2,004,107 + +0.5
2,004,107 + -0.3
2,004,107 + -1.8
2,004,107 + -0.2
2,004,114 + -0.4
2,004,114 + -0.3
2,004,114 + -1.8
2,004,114 + -0.2
2,004,114 + -0.5
2,004,114 + +0.7
2,004,323 + -2.3
2,004,358 - +0.7
2,004,358 - +1.3

Or see this region's nucleotide sequence