Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt asnB and Met_trna are separated by 379 nucleotides Met_trna and Leu_trna are separated by 10 nucleotides Leu_trna and Gln_trna are separated by 23 nucleotides Gln_trna and Gln_trna are separated by 34 nucleotides Gln_trna and Met_trna are separated by 15 nucleotides Met_trna and Gln_trna are separated by 48 nucleotides Gln_trna and Gln_trna are separated by 37 nucleotides Gln_trna and ubiF are separated by 153 nucleotides
MCAODC_26395: asnB - asparagine synthase B, at 2,001,453 to 2,003,117
asnB
MCAODC_26400: Met_trna - tRNA-Met(cat), at 2,003,497 to 2,003,573
_trna
MCAODC_26405: Leu_trna - tRNA-Leu(tag), at 2,003,584 to 2,003,668
_trna
MCAODC_26410: Gln_trna - tRNA-Gln(ttg), at 2,003,692 to 2,003,766
_trna
MCAODC_26415: Gln_trna - tRNA-Gln(ttg), at 2,003,801 to 2,003,875
_trna
MCAODC_26420: Met_trna - tRNA-Met(cat), at 2,003,891 to 2,003,967
_trna
MCAODC_26425: Gln_trna - tRNA-Gln(ctg), at 2,004,016 to 2,004,090
_trna
MCAODC_26430: Gln_trna - tRNA-Gln(ctg), at 2,004,128 to 2,004,202
_trna
MCAODC_26435: ubiF - 3-demethoxyubiquinol 3-hydroxylase, at 2,004,356 to 2,005,531
ubiF
Position (kb)
2003
2004 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2002.525 kb on - strand, within asnB at 2002.598 kb on + strand, within asnB at 2002.598 kb on + strand, within asnB at 2002.598 kb on + strand, within asnB at 2002.599 kb on - strand, within asnB at 2002.599 kb on - strand, within asnB at 2002.599 kb on - strand, within asnB at 2002.599 kb on - strand, within asnB at 2002.599 kb on - strand, within asnB at 2002.602 kb on + strand, within asnB at 2002.602 kb on + strand, within asnB at 2002.602 kb on + strand, within asnB at 2002.602 kb on + strand, within asnB at 2002.602 kb on + strand, within asnB at 2002.602 kb on + strand, within asnB at 2002.602 kb on + strand, within asnB at 2002.602 kb on + strand, within asnB at 2002.602 kb on + strand, within asnB at 2002.602 kb on + strand, within asnB at 2002.602 kb on + strand, within asnB at 2002.602 kb on + strand, within asnB at 2002.602 kb on + strand, within asnB at 2002.603 kb on - strand, within asnB at 2002.603 kb on - strand, within asnB at 2002.603 kb on - strand, within asnB at 2002.770 kb on + strand, within asnB at 2002.770 kb on + strand, within asnB at 2002.771 kb on - strand, within asnB at 2002.771 kb on - strand, within asnB at 2002.771 kb on - strand, within asnB at 2002.771 kb on - strand, within asnB at 2002.828 kb on - strand, within asnB at 2002.911 kb on + strand, within asnB at 2002.938 kb on + strand, within asnB at 2002.938 kb on + strand, within asnB at 2002.939 kb on - strand, within asnB at 2002.967 kb on - strand at 2003.110 kb on - strand at 2003.111 kb on + strand at 2003.111 kb on + strand at 2003.112 kb on - strand at 2003.112 kb on - strand at 2003.112 kb on - strand at 2003.112 kb on - strand at 2003.112 kb on - strand at 2003.112 kb on - strand at 2003.116 kb on - strand at 2003.261 kb on + strand at 2003.261 kb on + strand at 2003.262 kb on - strand at 2003.262 kb on - strand at 2003.281 kb on + strand at 2003.303 kb on - strand at 2003.303 kb on - strand at 2003.343 kb on + strand at 2003.350 kb on + strand at 2003.362 kb on - strand at 2003.385 kb on + strand at 2003.385 kb on + strand at 2003.959 kb on + strand, within Met_trna at 2004.107 kb on + strand at 2004.107 kb on + strand at 2004.107 kb on + strand at 2004.107 kb on + strand at 2004.114 kb on + strand at 2004.114 kb on + strand at 2004.114 kb on + strand at 2004.114 kb on + strand at 2004.114 kb on + strand at 2004.114 kb on + strand at 2004.323 kb on + strand at 2004.358 kb on - strand at 2004.358 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 2,002,525 - asnB MCAODC_26395 0.64 +0.4 2,002,598 + asnB MCAODC_26395 0.69 +0.6 2,002,598 + asnB MCAODC_26395 0.69 -0.1 2,002,598 + asnB MCAODC_26395 0.69 +0.8 2,002,599 - asnB MCAODC_26395 0.69 +0.4 2,002,599 - asnB MCAODC_26395 0.69 -0.9 2,002,599 - asnB MCAODC_26395 0.69 +1.4 2,002,599 - asnB MCAODC_26395 0.69 +1.7 2,002,599 - asnB MCAODC_26395 0.69 -1.7 2,002,602 + asnB MCAODC_26395 0.69 +0.6 2,002,602 + asnB MCAODC_26395 0.69 +1.2 2,002,602 + asnB MCAODC_26395 0.69 +0.8 2,002,602 + asnB MCAODC_26395 0.69 -0.9 2,002,602 + asnB MCAODC_26395 0.69 -0.2 2,002,602 + asnB MCAODC_26395 0.69 +0.6 2,002,602 + asnB MCAODC_26395 0.69 -0.2 2,002,602 + asnB MCAODC_26395 0.69 +0.4 2,002,602 + asnB MCAODC_26395 0.69 -0.0 2,002,602 + asnB MCAODC_26395 0.69 -0.3 2,002,602 + asnB MCAODC_26395 0.69 -2.7 2,002,602 + asnB MCAODC_26395 0.69 +2.0 2,002,602 + asnB MCAODC_26395 0.69 +1.9 2,002,603 - asnB MCAODC_26395 0.69 +0.3 2,002,603 - asnB MCAODC_26395 0.69 +0.9 2,002,603 - asnB MCAODC_26395 0.69 +1.5 2,002,770 + asnB MCAODC_26395 0.79 -0.6 2,002,770 + asnB MCAODC_26395 0.79 -0.5 2,002,771 - asnB MCAODC_26395 0.79 +0.5 2,002,771 - asnB MCAODC_26395 0.79 +0.7 2,002,771 - asnB MCAODC_26395 0.79 +0.6 2,002,771 - asnB MCAODC_26395 0.79 -0.3 2,002,828 - asnB MCAODC_26395 0.83 +1.3 2,002,911 + asnB MCAODC_26395 0.88 -0.2 2,002,938 + asnB MCAODC_26395 0.89 -2.5 2,002,938 + asnB MCAODC_26395 0.89 +2.0 2,002,939 - asnB MCAODC_26395 0.89 +1.0 2,002,967 - +2.1 2,003,110 - +0.3 2,003,111 + +0.8 2,003,111 + -1.8 2,003,112 - -0.2 2,003,112 - +0.1 2,003,112 - +0.8 2,003,112 - -1.6 2,003,112 - +1.3 2,003,112 - +1.5 2,003,116 - +0.9 2,003,261 + +0.1 2,003,261 + +2.0 2,003,262 - -1.9 2,003,262 - -1.3 2,003,281 + -0.3 2,003,303 - -1.2 2,003,303 - +1.5 2,003,343 + +0.6 2,003,350 + -0.8 2,003,362 - +2.6 2,003,385 + -0.6 2,003,385 + -1.9 2,003,959 + Met_trna MCAODC_26420 0.88 +0.3 2,004,107 + +0.5 2,004,107 + -0.3 2,004,107 + -1.8 2,004,107 + -0.2 2,004,114 + -0.4 2,004,114 + -0.3 2,004,114 + -1.8 2,004,114 + -0.2 2,004,114 + -0.5 2,004,114 + +0.7 2,004,323 + -2.3 2,004,358 - +0.7 2,004,358 - +1.3
Or see this region's nucleotide sequence