Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sucA and sdhB are separated by 300 nucleotides sdhB and sdhA are separated by 15 nucleotides
MCAODC_26145: sucA - 2-oxoglutarate dehydrogenase E1 component, at 1,942,645 to 1,945,446
sucA
MCAODC_26155: sdhB - succinate dehydrogenase iron-sulfur subunit SdhB, at 1,945,747 to 1,946,463
sdhB
MCAODC_26160: sdhA - succinate dehydrogenase flavoprotein subunit, at 1,946,479 to 1,948,245
sdhA
Position (kb)
1945
1946
1947 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1944.836 kb on - strand, within sucA at 1945.543 kb on - strand at 1945.543 kb on - strand at 1945.547 kb on - strand at 1945.581 kb on - strand at 1945.644 kb on - strand at 1945.741 kb on + strand at 1945.748 kb on + strand at 1945.756 kb on - strand at 1945.756 kb on - strand at 1945.779 kb on + strand at 1945.819 kb on + strand, within sdhB at 1945.820 kb on - strand, within sdhB at 1945.820 kb on - strand, within sdhB at 1945.842 kb on + strand, within sdhB at 1945.842 kb on + strand, within sdhB at 1945.843 kb on - strand, within sdhB at 1945.843 kb on - strand, within sdhB at 1945.843 kb on - strand, within sdhB at 1946.016 kb on + strand, within sdhB at 1946.017 kb on - strand, within sdhB at 1946.017 kb on - strand, within sdhB at 1946.017 kb on - strand, within sdhB at 1946.095 kb on - strand, within sdhB at 1946.106 kb on - strand, within sdhB at 1946.239 kb on - strand, within sdhB at 1946.396 kb on + strand at 1946.397 kb on - strand at 1946.397 kb on - strand at 1946.435 kb on + strand at 1946.436 kb on - strand at 1946.441 kb on + strand at 1946.483 kb on + strand at 1946.484 kb on - strand at 1946.484 kb on - strand at 1946.487 kb on + strand at 1946.487 kb on + strand at 1946.570 kb on + strand at 1946.625 kb on + strand at 1946.839 kb on - strand, within sdhA at 1946.839 kb on - strand, within sdhA at 1946.848 kb on - strand, within sdhA at 1946.848 kb on - strand, within sdhA at 1946.899 kb on - strand, within sdhA at 1946.899 kb on - strand, within sdhA at 1947.065 kb on - strand, within sdhA at 1947.182 kb on + strand, within sdhA
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 1,944,836 - sucA MCAODC_26145 0.78 -1.5 1,945,543 - -1.3 1,945,543 - -1.4 1,945,547 - -3.8 1,945,581 - +2.0 1,945,644 - -0.7 1,945,741 + +0.1 1,945,748 + -3.7 1,945,756 - -0.1 1,945,756 - +0.8 1,945,779 + -1.5 1,945,819 + sdhB MCAODC_26155 0.10 -2.6 1,945,820 - sdhB MCAODC_26155 0.10 +0.4 1,945,820 - sdhB MCAODC_26155 0.10 +1.6 1,945,842 + sdhB MCAODC_26155 0.13 -1.7 1,945,842 + sdhB MCAODC_26155 0.13 -1.0 1,945,843 - sdhB MCAODC_26155 0.13 -1.6 1,945,843 - sdhB MCAODC_26155 0.13 +0.4 1,945,843 - sdhB MCAODC_26155 0.13 +1.0 1,946,016 + sdhB MCAODC_26155 0.38 -1.9 1,946,017 - sdhB MCAODC_26155 0.38 +1.5 1,946,017 - sdhB MCAODC_26155 0.38 +1.5 1,946,017 - sdhB MCAODC_26155 0.38 -1.1 1,946,095 - sdhB MCAODC_26155 0.49 -0.7 1,946,106 - sdhB MCAODC_26155 0.50 -1.7 1,946,239 - sdhB MCAODC_26155 0.69 -1.5 1,946,396 + +0.6 1,946,397 - -0.2 1,946,397 - -0.1 1,946,435 + -0.4 1,946,436 - -0.1 1,946,441 + +2.6 1,946,483 + -1.6 1,946,484 - +0.5 1,946,484 - +1.7 1,946,487 + -1.9 1,946,487 + +1.0 1,946,570 + -1.0 1,946,625 + -1.0 1,946,839 - sdhA MCAODC_26160 0.20 +1.8 1,946,839 - sdhA MCAODC_26160 0.20 -1.8 1,946,848 - sdhA MCAODC_26160 0.21 -0.0 1,946,848 - sdhA MCAODC_26160 0.21 +0.6 1,946,899 - sdhA MCAODC_26160 0.24 +0.1 1,946,899 - sdhA MCAODC_26160 0.24 +1.0 1,947,065 - sdhA MCAODC_26160 0.33 -3.0 1,947,182 + sdhA MCAODC_26160 0.40 -1.0
Or see this region's nucleotide sequence