Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nadA and Lys_trna are separated by 277 nucleotides Lys_trna and Lys_trna are separated by 146 nucleotides Lys_trna and Val_trna are separated by 3 nucleotides Val_trna and Lys_trna are separated by 51 nucleotides Lys_trna and Val_trna are separated by 2 nucleotides Val_trna and Lys_trna are separated by 35 nucleotides Lys_trna and cpoB are separated by 164 nucleotides
MCAODC_25980: nadA - quinolinate synthase NadA, at 1,913,715 to 1,914,758
nadA
MCAODC_25985: Lys_trna - tRNA-Lys(ttt), at 1,915,036 to 1,915,111
_trna
MCAODC_25990: Lys_trna - tRNA-Lys(ttt), at 1,915,258 to 1,915,333
_trna
MCAODC_25995: Val_trna - tRNA-Val(tac), at 1,915,337 to 1,915,412
_trna
MCAODC_26000: Lys_trna - tRNA-Lys(ttt), at 1,915,464 to 1,915,539
_trna
MCAODC_26005: Val_trna - tRNA-Val(tac), at 1,915,542 to 1,915,617
_trna
MCAODC_26010: Lys_trna - tRNA-Lys(ttt), at 1,915,653 to 1,915,728
_trna
MCAODC_26015: cpoB - cell division protein CpoB, at 1,915,893 to 1,916,684
cpoB
Position (kb)
1915
1916 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1914.508 kb on - strand, within nadA at 1914.508 kb on - strand, within nadA at 1914.516 kb on - strand, within nadA at 1914.516 kb on - strand, within nadA at 1914.566 kb on + strand, within nadA at 1914.566 kb on + strand, within nadA at 1914.613 kb on + strand, within nadA at 1914.617 kb on - strand, within nadA at 1914.660 kb on + strand at 1914.782 kb on + strand at 1914.827 kb on - strand at 1914.887 kb on + strand at 1914.887 kb on + strand at 1914.918 kb on + strand at 1914.919 kb on - strand at 1914.919 kb on - strand at 1914.959 kb on + strand at 1915.123 kb on - strand at 1915.431 kb on - strand at 1915.431 kb on - strand at 1915.438 kb on - strand at 1915.438 kb on - strand at 1915.438 kb on - strand at 1915.438 kb on - strand at 1915.438 kb on - strand at 1915.458 kb on - strand at 1915.458 kb on - strand at 1915.458 kb on - strand at 1915.624 kb on - strand at 1915.624 kb on - strand at 1915.636 kb on - strand at 1915.642 kb on - strand at 1915.642 kb on - strand at 1915.642 kb on - strand at 1915.646 kb on - strand at 1915.646 kb on - strand at 1915.646 kb on - strand at 1915.781 kb on - strand at 1915.821 kb on + strand at 1915.821 kb on + strand at 1915.838 kb on + strand at 1915.839 kb on - strand at 1915.864 kb on + strand at 1915.864 kb on + strand at 1915.865 kb on - strand at 1915.894 kb on + strand at 1915.894 kb on + strand at 1915.894 kb on + strand at 1915.895 kb on - strand at 1915.895 kb on - strand at 1915.945 kb on + strand at 1915.949 kb on + strand at 1915.956 kb on - strand at 1916.080 kb on + strand, within cpoB at 1916.080 kb on + strand, within cpoB at 1916.080 kb on + strand, within cpoB at 1916.103 kb on - strand, within cpoB at 1916.103 kb on - strand, within cpoB at 1916.113 kb on + strand, within cpoB at 1916.114 kb on - strand, within cpoB at 1916.118 kb on + strand, within cpoB at 1916.152 kb on + strand, within cpoB at 1916.152 kb on + strand, within cpoB at 1916.153 kb on - strand, within cpoB at 1916.153 kb on - strand, within cpoB at 1916.237 kb on + strand, within cpoB at 1916.237 kb on + strand, within cpoB at 1916.238 kb on - strand, within cpoB at 1916.238 kb on - strand, within cpoB at 1916.238 kb on - strand, within cpoB at 1916.238 kb on - strand, within cpoB at 1916.248 kb on + strand, within cpoB at 1916.248 kb on + strand, within cpoB at 1916.249 kb on - strand, within cpoB at 1916.297 kb on + strand, within cpoB
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 1,914,508 - nadA MCAODC_25980 0.76 -0.9 1,914,508 - nadA MCAODC_25980 0.76 -0.8 1,914,516 - nadA MCAODC_25980 0.77 -0.2 1,914,516 - nadA MCAODC_25980 0.77 -3.0 1,914,566 + nadA MCAODC_25980 0.82 -0.0 1,914,566 + nadA MCAODC_25980 0.82 +0.3 1,914,613 + nadA MCAODC_25980 0.86 -2.7 1,914,617 - nadA MCAODC_25980 0.86 -3.9 1,914,660 + +1.2 1,914,782 + -2.5 1,914,827 - +1.2 1,914,887 + -0.1 1,914,887 + +2.2 1,914,918 + +0.7 1,914,919 - -1.6 1,914,919 - +0.9 1,914,959 + +0.3 1,915,123 - -0.6 1,915,431 - +0.7 1,915,431 - -0.6 1,915,438 - +1.1 1,915,438 - +0.2 1,915,438 - +0.2 1,915,438 - -0.3 1,915,438 - +0.8 1,915,458 - -0.9 1,915,458 - -0.8 1,915,458 - -4.4 1,915,624 - -0.7 1,915,624 - +0.9 1,915,636 - +0.1 1,915,642 - +0.2 1,915,642 - +0.3 1,915,642 - +0.3 1,915,646 - +0.4 1,915,646 - +1.8 1,915,646 - +3.2 1,915,781 - -1.2 1,915,821 + -1.3 1,915,821 + -1.1 1,915,838 + +2.3 1,915,839 - +0.4 1,915,864 + -0.2 1,915,864 + +0.3 1,915,865 - +1.3 1,915,894 + -0.4 1,915,894 + -0.7 1,915,894 + -0.0 1,915,895 - +1.2 1,915,895 - -0.6 1,915,945 + -0.3 1,915,949 + +1.7 1,915,956 - +0.0 1,916,080 + cpoB MCAODC_26015 0.24 -0.1 1,916,080 + cpoB MCAODC_26015 0.24 +0.7 1,916,080 + cpoB MCAODC_26015 0.24 -0.0 1,916,103 - cpoB MCAODC_26015 0.27 +1.2 1,916,103 - cpoB MCAODC_26015 0.27 -0.0 1,916,113 + cpoB MCAODC_26015 0.28 -2.4 1,916,114 - cpoB MCAODC_26015 0.28 -0.8 1,916,118 + cpoB MCAODC_26015 0.28 -1.3 1,916,152 + cpoB MCAODC_26015 0.33 +1.2 1,916,152 + cpoB MCAODC_26015 0.33 -2.1 1,916,153 - cpoB MCAODC_26015 0.33 -0.0 1,916,153 - cpoB MCAODC_26015 0.33 -0.1 1,916,237 + cpoB MCAODC_26015 0.43 +3.2 1,916,237 + cpoB MCAODC_26015 0.43 +0.0 1,916,238 - cpoB MCAODC_26015 0.44 +0.1 1,916,238 - cpoB MCAODC_26015 0.44 -0.3 1,916,238 - cpoB MCAODC_26015 0.44 +0.9 1,916,238 - cpoB MCAODC_26015 0.44 -0.2 1,916,248 + cpoB MCAODC_26015 0.45 +0.4 1,916,248 + cpoB MCAODC_26015 0.45 +0.3 1,916,249 - cpoB MCAODC_26015 0.45 -1.0 1,916,297 + cpoB MCAODC_26015 0.51 +0.5
Or see this region's nucleotide sequence