Strain Fitness in Escherichia coli ECRC101 around MCAODC_26000

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnadA and Lys_trna are separated by 277 nucleotidesLys_trna and Lys_trna are separated by 146 nucleotidesLys_trna and Val_trna are separated by 3 nucleotidesVal_trna and Lys_trna are separated by 51 nucleotidesLys_trna and Val_trna are separated by 2 nucleotidesVal_trna and Lys_trna are separated by 35 nucleotidesLys_trna and cpoB are separated by 164 nucleotides MCAODC_25980: nadA - quinolinate synthase NadA, at 1,913,715 to 1,914,758 nadA MCAODC_25985: Lys_trna - tRNA-Lys(ttt), at 1,915,036 to 1,915,111 _trna MCAODC_25990: Lys_trna - tRNA-Lys(ttt), at 1,915,258 to 1,915,333 _trna MCAODC_25995: Val_trna - tRNA-Val(tac), at 1,915,337 to 1,915,412 _trna MCAODC_26000: Lys_trna - tRNA-Lys(ttt), at 1,915,464 to 1,915,539 _trna MCAODC_26005: Val_trna - tRNA-Val(tac), at 1,915,542 to 1,915,617 _trna MCAODC_26010: Lys_trna - tRNA-Lys(ttt), at 1,915,653 to 1,915,728 _trna MCAODC_26015: cpoB - cell division protein CpoB, at 1,915,893 to 1,916,684 cpoB Position (kb) 1915 1916Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 1914.508 kb on - strand, within nadAat 1914.508 kb on - strand, within nadAat 1914.516 kb on - strand, within nadAat 1914.516 kb on - strand, within nadAat 1914.566 kb on + strand, within nadAat 1914.566 kb on + strand, within nadAat 1914.613 kb on + strand, within nadAat 1914.617 kb on - strand, within nadAat 1914.660 kb on + strandat 1914.782 kb on + strandat 1914.827 kb on - strandat 1914.887 kb on + strandat 1914.887 kb on + strandat 1914.918 kb on + strandat 1914.919 kb on - strandat 1914.919 kb on - strandat 1914.959 kb on + strandat 1915.123 kb on - strandat 1915.431 kb on - strandat 1915.431 kb on - strandat 1915.438 kb on - strandat 1915.438 kb on - strandat 1915.438 kb on - strandat 1915.438 kb on - strandat 1915.438 kb on - strandat 1915.458 kb on - strandat 1915.458 kb on - strandat 1915.458 kb on - strandat 1915.624 kb on - strandat 1915.624 kb on - strandat 1915.636 kb on - strandat 1915.642 kb on - strandat 1915.642 kb on - strandat 1915.642 kb on - strandat 1915.646 kb on - strandat 1915.646 kb on - strandat 1915.646 kb on - strandat 1915.781 kb on - strandat 1915.821 kb on + strandat 1915.821 kb on + strandat 1915.838 kb on + strandat 1915.839 kb on - strandat 1915.864 kb on + strandat 1915.864 kb on + strandat 1915.865 kb on - strandat 1915.894 kb on + strandat 1915.894 kb on + strandat 1915.894 kb on + strandat 1915.895 kb on - strandat 1915.895 kb on - strandat 1915.945 kb on + strandat 1915.949 kb on + strandat 1915.956 kb on - strandat 1916.080 kb on + strand, within cpoBat 1916.080 kb on + strand, within cpoBat 1916.080 kb on + strand, within cpoBat 1916.103 kb on - strand, within cpoBat 1916.103 kb on - strand, within cpoBat 1916.113 kb on + strand, within cpoBat 1916.114 kb on - strand, within cpoBat 1916.118 kb on + strand, within cpoBat 1916.152 kb on + strand, within cpoBat 1916.152 kb on + strand, within cpoBat 1916.153 kb on - strand, within cpoBat 1916.153 kb on - strand, within cpoBat 1916.237 kb on + strand, within cpoBat 1916.237 kb on + strand, within cpoBat 1916.238 kb on - strand, within cpoBat 1916.238 kb on - strand, within cpoBat 1916.238 kb on - strand, within cpoBat 1916.238 kb on - strand, within cpoBat 1916.248 kb on + strand, within cpoBat 1916.248 kb on + strand, within cpoBat 1916.249 kb on - strand, within cpoBat 1916.297 kb on + strand, within cpoB

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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1,914,508 - nadA MCAODC_25980 0.76 -0.9
1,914,508 - nadA MCAODC_25980 0.76 -0.8
1,914,516 - nadA MCAODC_25980 0.77 -0.2
1,914,516 - nadA MCAODC_25980 0.77 -3.0
1,914,566 + nadA MCAODC_25980 0.82 -0.0
1,914,566 + nadA MCAODC_25980 0.82 +0.3
1,914,613 + nadA MCAODC_25980 0.86 -2.7
1,914,617 - nadA MCAODC_25980 0.86 -3.9
1,914,660 + +1.2
1,914,782 + -2.5
1,914,827 - +1.2
1,914,887 + -0.1
1,914,887 + +2.2
1,914,918 + +0.7
1,914,919 - -1.6
1,914,919 - +0.9
1,914,959 + +0.3
1,915,123 - -0.6
1,915,431 - +0.7
1,915,431 - -0.6
1,915,438 - +1.1
1,915,438 - +0.2
1,915,438 - +0.2
1,915,438 - -0.3
1,915,438 - +0.8
1,915,458 - -0.9
1,915,458 - -0.8
1,915,458 - -4.4
1,915,624 - -0.7
1,915,624 - +0.9
1,915,636 - +0.1
1,915,642 - +0.2
1,915,642 - +0.3
1,915,642 - +0.3
1,915,646 - +0.4
1,915,646 - +1.8
1,915,646 - +3.2
1,915,781 - -1.2
1,915,821 + -1.3
1,915,821 + -1.1
1,915,838 + +2.3
1,915,839 - +0.4
1,915,864 + -0.2
1,915,864 + +0.3
1,915,865 - +1.3
1,915,894 + -0.4
1,915,894 + -0.7
1,915,894 + -0.0
1,915,895 - +1.2
1,915,895 - -0.6
1,915,945 + -0.3
1,915,949 + +1.7
1,915,956 - +0.0
1,916,080 + cpoB MCAODC_26015 0.24 -0.1
1,916,080 + cpoB MCAODC_26015 0.24 +0.7
1,916,080 + cpoB MCAODC_26015 0.24 -0.0
1,916,103 - cpoB MCAODC_26015 0.27 +1.2
1,916,103 - cpoB MCAODC_26015 0.27 -0.0
1,916,113 + cpoB MCAODC_26015 0.28 -2.4
1,916,114 - cpoB MCAODC_26015 0.28 -0.8
1,916,118 + cpoB MCAODC_26015 0.28 -1.3
1,916,152 + cpoB MCAODC_26015 0.33 +1.2
1,916,152 + cpoB MCAODC_26015 0.33 -2.1
1,916,153 - cpoB MCAODC_26015 0.33 -0.0
1,916,153 - cpoB MCAODC_26015 0.33 -0.1
1,916,237 + cpoB MCAODC_26015 0.43 +3.2
1,916,237 + cpoB MCAODC_26015 0.43 +0.0
1,916,238 - cpoB MCAODC_26015 0.44 +0.1
1,916,238 - cpoB MCAODC_26015 0.44 -0.3
1,916,238 - cpoB MCAODC_26015 0.44 +0.9
1,916,238 - cpoB MCAODC_26015 0.44 -0.2
1,916,248 + cpoB MCAODC_26015 0.45 +0.4
1,916,248 + cpoB MCAODC_26015 0.45 +0.3
1,916,249 - cpoB MCAODC_26015 0.45 -1.0
1,916,297 + cpoB MCAODC_26015 0.51 +0.5

Or see this region's nucleotide sequence