Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bet and exoD overlap by 4 nucleotides exoD and MCAODC_25830 overlap by 4 nucleotides MCAODC_25830 and MCAODC_25835 overlap by 28 nucleotides MCAODC_25835 and MCAODC_25840 overlap by 4 nucleotides MCAODC_25840 and MCAODC_25845 overlap by 4 nucleotides MCAODC_25845 and MCAODC_25850 are separated by 98 nucleotides MCAODC_25850 and MCAODC_25855 are separated by 210 nucleotides
MCAODC_25820: bet - phage recombination protein Bet, at 1,884,703 to 1,885,350
bet
MCAODC_25825: exoD - Putative uncharacterized protein ExoD, at 1,885,347 to 1,886,027
exoD
MCAODC_25830: MCAODC_25830 - cruciferin, at 1,886,024 to 1,886,206
_25830
MCAODC_25835: MCAODC_25835 - DUF1382 domain-containing protein, at 1,886,179 to 1,886,370
_25835
MCAODC_25840: MCAODC_25840 - DUF1382 domain-containing protein, at 1,886,367 to 1,886,450
_25840
MCAODC_25845: MCAODC_25845 - Cell division protein ZapA, at 1,886,447 to 1,886,662
_25845
MCAODC_25850: MCAODC_25850 - conjugal transfer protein TraR, at 1,886,761 to 1,886,982
_25850
MCAODC_25855: MCAODC_25855 - hypothetical protein, at 1,887,193 to 1,887,795
_25855
Position (kb) 1886
1887 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1885.107 kb on + strand, within bet at 1885.469 kb on - strand, within exoD at 1885.565 kb on + strand, within exoD at 1885.647 kb on - strand, within exoD at 1885.807 kb on - strand, within exoD at 1885.807 kb on - strand, within exoD at 1885.825 kb on - strand, within exoD at 1885.825 kb on - strand, within exoD at 1885.903 kb on - strand, within exoD at 1885.903 kb on - strand, within exoD at 1885.903 kb on - strand, within exoD at 1885.903 kb on - strand, within exoD at 1886.157 kb on + strand, within MCAODC_25830 at 1886.158 kb on - strand, within MCAODC_25830 at 1886.158 kb on - strand, within MCAODC_25830 at 1886.161 kb on + strand, within MCAODC_25830 at 1886.161 kb on + strand, within MCAODC_25830 at 1886.161 kb on + strand, within MCAODC_25830 at 1886.161 kb on + strand, within MCAODC_25830 at 1886.292 kb on + strand, within MCAODC_25835 at 1886.292 kb on + strand, within MCAODC_25835 at 1886.408 kb on - strand, within MCAODC_25840 at 1886.421 kb on - strand, within MCAODC_25840 at 1887.117 kb on - strand at 1887.165 kb on - strand at 1887.183 kb on - strand at 1887.190 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 1,885,107 + bet MCAODC_25820 0.62 +0.5 1,885,469 - exoD MCAODC_25825 0.18 +1.0 1,885,565 + exoD MCAODC_25825 0.32 -1.0 1,885,647 - exoD MCAODC_25825 0.44 -0.6 1,885,807 - exoD MCAODC_25825 0.68 -0.7 1,885,807 - exoD MCAODC_25825 0.68 -0.6 1,885,825 - exoD MCAODC_25825 0.70 +1.4 1,885,825 - exoD MCAODC_25825 0.70 -3.0 1,885,903 - exoD MCAODC_25825 0.82 +0.7 1,885,903 - exoD MCAODC_25825 0.82 -0.8 1,885,903 - exoD MCAODC_25825 0.82 +2.0 1,885,903 - exoD MCAODC_25825 0.82 -1.3 1,886,157 + MCAODC_25830 0.73 +1.0 1,886,158 - MCAODC_25830 0.73 +0.1 1,886,158 - MCAODC_25830 0.73 +0.4 1,886,161 + MCAODC_25830 0.75 -4.3 1,886,161 + MCAODC_25830 0.75 -1.0 1,886,161 + MCAODC_25830 0.75 -1.8 1,886,161 + MCAODC_25830 0.75 -0.1 1,886,292 + MCAODC_25835 0.59 -0.0 1,886,292 + MCAODC_25835 0.59 +0.3 1,886,408 - MCAODC_25840 0.49 -0.6 1,886,421 - MCAODC_25840 0.64 +1.5 1,887,117 - +0.7 1,887,165 - +0.6 1,887,183 - +0.8 1,887,190 - +1.3
Or see this region's nucleotide sequence