Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_21450 and rusA are separated by 8 nucleotides rusA and MCAODC_21460 are separated by 12 nucleotides MCAODC_21460 and MCAODC_21465 are separated by 1 nucleotides MCAODC_21465 and MCAODC_21470 are separated by 69 nucleotides MCAODC_21470 and hokD1 are separated by 220 nucleotides
MCAODC_21450: MCAODC_21450 - DUF1133 domain-containing protein, at 1,083,516 to 1,084,046
_21450
MCAODC_21455: rusA - Endodeoxyribonuclease RusA, at 1,084,055 to 1,084,414
rusA
MCAODC_21460: MCAODC_21460 - DUF968 domain-containing protein, at 1,084,427 to 1,085,473
_21460
MCAODC_21465: MCAODC_21465 - hypothetical protein, at 1,085,475 to 1,085,753
_21465
MCAODC_21470: MCAODC_21470 - Hypothetical rem protein, at 1,085,823 to 1,086,080
_21470
MCAODC_21475: hokD1 - Protein HokD, at 1,086,301 to 1,086,513
hokD1
Position (kb)
1084
1085
1086 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1083.457 kb on - strand at 1083.541 kb on - strand at 1083.544 kb on + strand at 1083.545 kb on - strand at 1083.545 kb on - strand at 1083.545 kb on - strand at 1083.545 kb on - strand at 1083.651 kb on - strand, within MCAODC_21450 at 1083.671 kb on - strand, within MCAODC_21450 at 1083.687 kb on - strand, within MCAODC_21450 at 1083.758 kb on + strand, within MCAODC_21450 at 1083.759 kb on - strand, within MCAODC_21450 at 1083.867 kb on - strand, within MCAODC_21450 at 1083.941 kb on - strand, within MCAODC_21450 at 1083.941 kb on - strand, within MCAODC_21450 at 1083.941 kb on - strand, within MCAODC_21450 at 1084.006 kb on - strand at 1084.041 kb on + strand at 1084.047 kb on - strand at 1084.047 kb on - strand at 1084.047 kb on - strand at 1084.051 kb on - strand at 1084.338 kb on - strand, within rusA at 1084.344 kb on + strand, within rusA at 1084.832 kb on + strand, within MCAODC_21460 at 1084.832 kb on + strand, within MCAODC_21460 at 1084.860 kb on - strand, within MCAODC_21460 at 1084.991 kb on + strand, within MCAODC_21460 at 1085.016 kb on - strand, within MCAODC_21460 at 1085.419 kb on + strand at 1085.463 kb on - strand at 1085.463 kb on - strand at 1085.594 kb on - strand, within MCAODC_21465 at 1085.594 kb on - strand, within MCAODC_21465 at 1085.812 kb on - strand at 1085.947 kb on - strand, within MCAODC_21470 at 1085.987 kb on + strand, within MCAODC_21470 at 1085.990 kb on + strand, within MCAODC_21470 at 1086.033 kb on - strand, within MCAODC_21470 at 1086.033 kb on - strand, within MCAODC_21470 at 1086.103 kb on + strand at 1086.456 kb on + strand, within hokD1
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 1,083,457 - -1.2 1,083,541 - -1.7 1,083,544 + +0.1 1,083,545 - -0.7 1,083,545 - +1.7 1,083,545 - -0.2 1,083,545 - +1.0 1,083,651 - MCAODC_21450 0.25 +0.1 1,083,671 - MCAODC_21450 0.29 -2.4 1,083,687 - MCAODC_21450 0.32 +0.2 1,083,758 + MCAODC_21450 0.46 +1.1 1,083,759 - MCAODC_21450 0.46 +0.7 1,083,867 - MCAODC_21450 0.66 +0.2 1,083,941 - MCAODC_21450 0.80 -0.1 1,083,941 - MCAODC_21450 0.80 -0.7 1,083,941 - MCAODC_21450 0.80 -0.6 1,084,006 - +0.8 1,084,041 + +0.8 1,084,047 - -1.9 1,084,047 - +0.2 1,084,047 - -1.3 1,084,051 - -3.4 1,084,338 - rusA MCAODC_21455 0.79 +1.1 1,084,344 + rusA MCAODC_21455 0.80 +0.5 1,084,832 + MCAODC_21460 0.39 -3.2 1,084,832 + MCAODC_21460 0.39 -1.6 1,084,860 - MCAODC_21460 0.41 +1.2 1,084,991 + MCAODC_21460 0.54 -1.8 1,085,016 - MCAODC_21460 0.56 -0.2 1,085,419 + +2.0 1,085,463 - +1.5 1,085,463 - -0.4 1,085,594 - MCAODC_21465 0.43 +0.3 1,085,594 - MCAODC_21465 0.43 -1.5 1,085,812 - +0.5 1,085,947 - MCAODC_21470 0.48 +1.1 1,085,987 + MCAODC_21470 0.64 -0.2 1,085,990 + MCAODC_21470 0.65 +1.1 1,086,033 - MCAODC_21470 0.81 +1.6 1,086,033 - MCAODC_21470 0.81 +0.5 1,086,103 + +2.3 1,086,456 + hokD1 MCAODC_21475 0.73 +1.6
Or see this region's nucleotide sequence