Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_20950 and MCAODC_20955 overlap by 11 nucleotides MCAODC_20955 and oRF82 overlap by 4 nucleotides oRF82 and oRF81 are separated by 2 nucleotides oRF81 and oRF80 overlap by 1 nucleotides oRF80 and MCAODC_20975 are separated by 63 nucleotides MCAODC_20975 and MCAODC_20980 overlap by 4 nucleotides MCAODC_20980 and MCAODC_20985 are separated by 10 nucleotides
MCAODC_20950: MCAODC_20950 - replication protein A, at 996,517 to 998,799
_20950
MCAODC_20955: MCAODC_20955 - Uncharacterized protein in GpA 5'region, at 998,789 to 999,064
_20955
MCAODC_20960: oRF82 - Uncharacterized protein in gpA 5'region, at 999,061 to 999,285
oRF82
MCAODC_20965: oRF81 - Uncharacterized protein in GpA 5'region, at 999,288 to 999,587
oRF81
MCAODC_20970: oRF80 - Uncharacterized protein in GpA 5'region, at 999,587 to 999,811
oRF80
MCAODC_20975: MCAODC_20975 - Replication gene B protein, at 999,875 to 1,000,375
_20975
MCAODC_20980: MCAODC_20980 - Phage protein B, at 1,000,372 to 1,000,542
_20980
MCAODC_20985: MCAODC_20985 - Regulatory phage protein cox, at 1,000,553 to 1,000,828
_20985
Position (kb) 999
1000 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 998.351 kb on + strand, within MCAODC_20950 at 998.351 kb on + strand, within MCAODC_20950 at 998.351 kb on + strand, within MCAODC_20950 at 998.352 kb on - strand, within MCAODC_20950 at 998.352 kb on - strand, within MCAODC_20950 at 998.429 kb on + strand, within MCAODC_20950 at 998.430 kb on - strand, within MCAODC_20950 at 998.455 kb on + strand, within MCAODC_20950 at 998.456 kb on - strand, within MCAODC_20950 at 998.456 kb on - strand, within MCAODC_20950 at 998.543 kb on + strand, within MCAODC_20950 at 998.544 kb on - strand, within MCAODC_20950 at 998.566 kb on - strand, within MCAODC_20950 at 998.673 kb on + strand at 998.673 kb on + strand at 998.673 kb on + strand at 998.720 kb on + strand at 998.720 kb on + strand at 998.721 kb on - strand at 998.721 kb on - strand at 998.721 kb on - strand at 998.721 kb on - strand at 998.799 kb on + strand at 998.799 kb on + strand at 998.799 kb on + strand at 998.800 kb on - strand at 998.800 kb on - strand at 998.921 kb on + strand, within MCAODC_20955 at 998.922 kb on - strand, within MCAODC_20955 at 998.922 kb on - strand, within MCAODC_20955 at 998.935 kb on - strand, within MCAODC_20955 at 999.033 kb on + strand, within MCAODC_20955 at 999.059 kb on - strand at 999.272 kb on - strand at 999.300 kb on - strand at 999.331 kb on + strand, within oRF81 at 999.404 kb on + strand, within oRF81 at 999.564 kb on + strand at 1000.217 kb on + strand, within MCAODC_20975 at 1000.217 kb on + strand, within MCAODC_20975 at 1000.269 kb on + strand, within MCAODC_20975 at 1000.270 kb on - strand, within MCAODC_20975 at 1000.273 kb on - strand, within MCAODC_20975 at 1000.273 kb on - strand, within MCAODC_20975 at 1000.282 kb on + strand, within MCAODC_20975 at 1000.283 kb on - strand, within MCAODC_20975 at 1000.330 kb on + strand at 1000.417 kb on + strand, within MCAODC_20980 at 1000.514 kb on - strand, within MCAODC_20980 at 1000.524 kb on + strand, within MCAODC_20980 at 1000.524 kb on + strand, within MCAODC_20980 at 1000.543 kb on - strand at 1000.575 kb on - strand at 1000.575 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 998,351 + MCAODC_20950 0.80 +0.5 998,351 + MCAODC_20950 0.80 -0.3 998,351 + MCAODC_20950 0.80 +1.0 998,352 - MCAODC_20950 0.80 +0.1 998,352 - MCAODC_20950 0.80 -0.4 998,429 + MCAODC_20950 0.84 +1.2 998,430 - MCAODC_20950 0.84 -1.1 998,455 + MCAODC_20950 0.85 -0.6 998,456 - MCAODC_20950 0.85 -2.3 998,456 - MCAODC_20950 0.85 -1.3 998,543 + MCAODC_20950 0.89 +0.1 998,544 - MCAODC_20950 0.89 +0.1 998,566 - MCAODC_20950 0.90 +0.5 998,673 + -3.1 998,673 + -0.6 998,673 + +1.6 998,720 + +1.2 998,720 + +0.6 998,721 - -0.1 998,721 - +0.6 998,721 - -1.1 998,721 - -0.8 998,799 + -0.7 998,799 + +1.0 998,799 + -2.1 998,800 - -0.6 998,800 - -0.4 998,921 + MCAODC_20955 0.48 +1.1 998,922 - MCAODC_20955 0.48 -0.9 998,922 - MCAODC_20955 0.48 +1.4 998,935 - MCAODC_20955 0.53 +0.1 999,033 + MCAODC_20955 0.88 +0.1 999,059 - +0.1 999,272 - +0.9 999,300 - -2.8 999,331 + oRF81 MCAODC_20965 0.14 +1.3 999,404 + oRF81 MCAODC_20965 0.39 -3.3 999,564 + +2.4 1,000,217 + MCAODC_20975 0.68 +0.6 1,000,217 + MCAODC_20975 0.68 -1.8 1,000,269 + MCAODC_20975 0.79 -0.3 1,000,270 - MCAODC_20975 0.79 -0.4 1,000,273 - MCAODC_20975 0.79 +0.9 1,000,273 - MCAODC_20975 0.79 -1.3 1,000,282 + MCAODC_20975 0.81 -1.2 1,000,283 - MCAODC_20975 0.81 +0.0 1,000,330 + -1.4 1,000,417 + MCAODC_20980 0.26 -0.3 1,000,514 - MCAODC_20980 0.83 +1.3 1,000,524 + MCAODC_20980 0.89 -0.1 1,000,524 + MCAODC_20980 0.89 -1.0 1,000,543 - -2.1 1,000,575 - +0.9 1,000,575 - -0.9
Or see this region's nucleotide sequence