Strain Fitness in Escherichia coli ECRC101 around MCAODC_20435

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisC and hisB overlap by 1 nucleotideshisB and hisH overlap by 1 nucleotideshisH and hisA overlap by 1 nucleotides MCAODC_20430: hisC - histidinol-phosphate transaminase, at 892,353 to 893,423 hisC MCAODC_20435: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 893,423 to 894,490 hisB MCAODC_20440: hisH - imidazole glycerol phosphate synthase subunit HisH, at 894,490 to 895,080 hisH MCAODC_20445: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 895,080 to 895,817 hisA Position (kb) 893 894 895Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 892.468 kb on - strand, within hisCat 892.494 kb on - strand, within hisCat 892.560 kb on + strand, within hisCat 892.561 kb on - strand, within hisCat 892.614 kb on - strand, within hisCat 892.614 kb on - strand, within hisCat 892.680 kb on + strand, within hisCat 892.762 kb on + strand, within hisCat 892.763 kb on - strand, within hisCat 892.803 kb on + strand, within hisCat 892.804 kb on - strand, within hisCat 892.911 kb on + strand, within hisCat 892.912 kb on - strand, within hisCat 892.912 kb on - strand, within hisCat 892.912 kb on - strand, within hisCat 892.912 kb on - strand, within hisCat 892.912 kb on - strand, within hisCat 892.973 kb on + strand, within hisCat 893.027 kb on + strand, within hisCat 893.027 kb on + strand, within hisCat 893.028 kb on - strand, within hisCat 893.028 kb on - strand, within hisCat 893.168 kb on + strand, within hisCat 893.247 kb on + strand, within hisCat 893.248 kb on - strand, within hisCat 893.248 kb on - strand, within hisCat 893.310 kb on - strand, within hisCat 893.405 kb on - strandat 893.677 kb on - strand, within hisBat 893.798 kb on + strand, within hisBat 893.798 kb on + strand, within hisBat 893.941 kb on + strand, within hisBat 893.945 kb on - strand, within hisBat 894.004 kb on + strand, within hisBat 894.263 kb on + strand, within hisBat 894.265 kb on + strand, within hisBat 894.266 kb on - strand, within hisBat 894.341 kb on + strand, within hisBat 894.341 kb on + strand, within hisBat 894.341 kb on + strand, within hisBat 894.342 kb on - strand, within hisBat 894.379 kb on + strand, within hisBat 894.400 kb on - strandat 894.488 kb on + strandat 894.960 kb on + strand, within hisHat 894.960 kb on + strand, within hisHat 894.960 kb on + strand, within hisHat 894.964 kb on + strand, within hisHat 894.965 kb on - strand, within hisHat 895.007 kb on - strand, within hisHat 895.275 kb on - strand, within hisA

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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892,468 - hisC MCAODC_20430 0.11 -1.3
892,494 - hisC MCAODC_20430 0.13 -1.1
892,560 + hisC MCAODC_20430 0.19 -3.4
892,561 - hisC MCAODC_20430 0.19 -2.7
892,614 - hisC MCAODC_20430 0.24 -4.3
892,614 - hisC MCAODC_20430 0.24 -1.9
892,680 + hisC MCAODC_20430 0.31 -4.5
892,762 + hisC MCAODC_20430 0.38 -2.1
892,763 - hisC MCAODC_20430 0.38 -3.2
892,803 + hisC MCAODC_20430 0.42 -3.3
892,804 - hisC MCAODC_20430 0.42 -2.9
892,911 + hisC MCAODC_20430 0.52 -0.8
892,912 - hisC MCAODC_20430 0.52 -4.3
892,912 - hisC MCAODC_20430 0.52 -3.0
892,912 - hisC MCAODC_20430 0.52 -3.3
892,912 - hisC MCAODC_20430 0.52 -1.7
892,912 - hisC MCAODC_20430 0.52 -2.3
892,973 + hisC MCAODC_20430 0.58 -1.9
893,027 + hisC MCAODC_20430 0.63 -2.3
893,027 + hisC MCAODC_20430 0.63 -3.1
893,028 - hisC MCAODC_20430 0.63 -1.4
893,028 - hisC MCAODC_20430 0.63 -0.1
893,168 + hisC MCAODC_20430 0.76 -2.5
893,247 + hisC MCAODC_20430 0.83 -1.7
893,248 - hisC MCAODC_20430 0.84 -3.1
893,248 - hisC MCAODC_20430 0.84 -3.1
893,310 - hisC MCAODC_20430 0.89 -3.8
893,405 - -1.3
893,677 - hisB MCAODC_20435 0.24 -2.5
893,798 + hisB MCAODC_20435 0.35 -0.1
893,798 + hisB MCAODC_20435 0.35 -0.4
893,941 + hisB MCAODC_20435 0.49 -2.5
893,945 - hisB MCAODC_20435 0.49 -1.3
894,004 + hisB MCAODC_20435 0.54 -2.4
894,263 + hisB MCAODC_20435 0.79 -4.8
894,265 + hisB MCAODC_20435 0.79 -1.0
894,266 - hisB MCAODC_20435 0.79 -3.7
894,341 + hisB MCAODC_20435 0.86 -3.8
894,341 + hisB MCAODC_20435 0.86 -3.8
894,341 + hisB MCAODC_20435 0.86 -0.4
894,342 - hisB MCAODC_20435 0.86 -2.5
894,379 + hisB MCAODC_20435 0.90 -3.8
894,400 - -2.6
894,488 + -3.2
894,960 + hisH MCAODC_20440 0.80 +0.5
894,960 + hisH MCAODC_20440 0.80 -0.4
894,960 + hisH MCAODC_20440 0.80 -0.6
894,964 + hisH MCAODC_20440 0.80 -3.5
894,965 - hisH MCAODC_20440 0.80 -1.6
895,007 - hisH MCAODC_20440 0.87 -2.5
895,275 - hisA MCAODC_20445 0.26 +0.0

Or see this region's nucleotide sequence