Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt espR3 and espR4 are separated by 818 nucleotides espR4 and fliE are separated by 348 nucleotides fliE and fliF are separated by 214 nucleotides
MCAODC_19665: espR3 - Type III secretion system effector espR3, at 763,009 to 764,148
espR3
MCAODC_19670: espR4 - T3SS effector leucine-rich repeat protein EspR4, at 764,967 to 766,106
espR4
MCAODC_19675: fliE - flagellar hook-basal body complex protein FliE, at 766,455 to 766,769
fliE
MCAODC_19680: fliF - flagellar M-ring protein FliF, at 766,984 to 768,642
fliF
Position (kb) 764
765
766
767 Strain fitness (log2 ratio) -2
-1
0
1
2 at 764.245 kb on + strand at 764.557 kb on - strand at 764.597 kb on - strand at 765.562 kb on - strand, within espR4 at 765.666 kb on - strand, within espR4 at 766.327 kb on + strand at 766.328 kb on - strand at 766.330 kb on - strand at 766.437 kb on - strand at 766.437 kb on - strand at 766.456 kb on + strand at 766.456 kb on + strand at 766.457 kb on - strand at 766.457 kb on - strand at 766.457 kb on - strand at 766.457 kb on - strand at 766.474 kb on - strand at 766.474 kb on - strand at 766.500 kb on - strand, within fliE at 766.628 kb on + strand, within fliE at 766.674 kb on - strand, within fliE at 766.674 kb on - strand, within fliE at 766.805 kb on - strand at 766.867 kb on - strand at 766.888 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 764,245 + +0.2 764,557 - +0.6 764,597 - -0.3 765,562 - espR4 MCAODC_19670 0.52 -0.1 765,666 - espR4 MCAODC_19670 0.61 -0.8 766,327 + +0.5 766,328 - -0.3 766,330 - -0.0 766,437 - -1.8 766,437 - -0.2 766,456 + -1.7 766,456 + +0.8 766,457 - +2.3 766,457 - +1.2 766,457 - +0.8 766,457 - -0.1 766,474 - -0.8 766,474 - +1.9 766,500 - fliE MCAODC_19675 0.14 +1.0 766,628 + fliE MCAODC_19675 0.55 -2.5 766,674 - fliE MCAODC_19675 0.70 +0.9 766,674 - fliE MCAODC_19675 0.70 -0.6 766,805 - -0.3 766,867 - -1.8 766,888 + +1.2
Or see this region's nucleotide sequence