Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tra5 and tnp-IS629 overlap by 1 nucleotides tnp-IS629 and gpA1 are separated by 170 nucleotides gpA1 and cysE are separated by 43 nucleotides cysE and MCAODC_19505 are separated by 156 nucleotides
MCAODC_19480: tra5 - IS3 family transposase, at 732,866 to 733,555
tra5
MCAODC_19485: tnp-IS629 - IS3 family IS629 transposase, at 733,555 to 733,881
tnp-IS629
MCAODC_19490: gpA1 - Phage terminase, large subunit GpA, at 734,052 to 734,468
gpA1
MCAODC_19500: cysE - serine acetyltransferase, at 734,512 to 735,084
cysE
MCAODC_19505: MCAODC_19505 - oxidoreductase, at 735,241 to 735,729
_19505
Position (kb) 734
735 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 734.037 kb on - strand at 734.069 kb on + strand at 734.069 kb on + strand at 734.180 kb on + strand, within gpA1 at 734.424 kb on - strand, within gpA1 at 734.431 kb on + strand at 734.548 kb on - strand at 734.722 kb on - strand, within cysE at 734.732 kb on - strand, within cysE at 734.887 kb on + strand, within cysE at 735.101 kb on - strand at 735.187 kb on + strand at 735.232 kb on + strand at 735.232 kb on + strand at 735.232 kb on + strand at 735.232 kb on + strand at 735.233 kb on - strand at 735.295 kb on - strand, within MCAODC_19505 at 735.452 kb on + strand, within MCAODC_19505 at 735.452 kb on + strand, within MCAODC_19505 at 735.452 kb on + strand, within MCAODC_19505 at 735.453 kb on - strand, within MCAODC_19505 at 735.453 kb on - strand, within MCAODC_19505
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 734,037 - +1.0 734,069 + +1.0 734,069 + +0.5 734,180 + gpA1 MCAODC_19490 0.31 +1.0 734,424 - gpA1 MCAODC_19490 0.89 -2.1 734,431 + +0.5 734,548 - -1.2 734,722 - cysE MCAODC_19500 0.37 +1.0 734,732 - cysE MCAODC_19500 0.38 +1.2 734,887 + cysE MCAODC_19500 0.65 -0.3 735,101 - -2.1 735,187 + -1.6 735,232 + +0.2 735,232 + -2.8 735,232 + -0.2 735,232 + +2.1 735,233 - -0.8 735,295 - MCAODC_19505 0.11 -1.0 735,452 + MCAODC_19505 0.43 +1.7 735,452 + MCAODC_19505 0.43 -0.5 735,452 + MCAODC_19505 0.43 -0.3 735,453 - MCAODC_19505 0.43 +0.7 735,453 - MCAODC_19505 0.43 -2.8
Or see this region's nucleotide sequence