Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aspS and yecD are separated by 309 nucleotides yecD and yecE overlap by 4 nucleotides yecE and yecN are separated by 52 nucleotides yecN and cmoA are separated by 40 nucleotides
MCAODC_19160: aspS - aspartate--tRNA ligase, at 679,947 to 681,719
aspS
MCAODC_19165: yecD - hydrolase, at 682,029 to 682,595
yecD
MCAODC_19170: yecE - UPF0759 protein YecE, at 682,592 to 683,410
yecE
MCAODC_19175: yecN - Inner membrane protein YecN, at 683,463 to 683,858
yecN
MCAODC_19180: cmoA - carboxy-S-adenosyl-L-methionine synthase CmoA, at 683,899 to 684,642
cmoA
Position (kb) 682
683
684 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 681.886 kb on + strand at 681.887 kb on - strand at 681.888 kb on + strand at 681.923 kb on + strand at 681.924 kb on - strand at 681.926 kb on - strand at 681.926 kb on - strand at 682.040 kb on + strand at 682.040 kb on + strand at 682.040 kb on + strand at 682.040 kb on + strand at 682.040 kb on + strand at 682.041 kb on - strand at 682.041 kb on - strand at 682.041 kb on - strand at 682.090 kb on + strand, within yecD at 682.090 kb on + strand, within yecD at 682.090 kb on + strand, within yecD at 682.090 kb on + strand, within yecD at 682.112 kb on + strand, within yecD at 682.113 kb on - strand, within yecD at 682.126 kb on + strand, within yecD at 682.126 kb on + strand, within yecD at 682.126 kb on + strand, within yecD at 682.127 kb on - strand, within yecD at 682.127 kb on - strand, within yecD at 682.127 kb on - strand, within yecD at 682.127 kb on - strand, within yecD at 682.130 kb on + strand, within yecD at 682.325 kb on - strand, within yecD at 682.394 kb on + strand, within yecD at 682.394 kb on + strand, within yecD at 682.395 kb on - strand, within yecD at 682.395 kb on - strand, within yecD at 682.399 kb on + strand, within yecD at 682.399 kb on + strand, within yecD at 682.399 kb on + strand, within yecD at 682.400 kb on - strand, within yecD at 682.400 kb on - strand, within yecD at 682.405 kb on + strand, within yecD at 682.405 kb on + strand, within yecD at 682.405 kb on + strand, within yecD at 682.405 kb on + strand, within yecD at 682.405 kb on + strand, within yecD at 682.405 kb on + strand, within yecD at 682.405 kb on + strand, within yecD at 682.406 kb on - strand, within yecD at 682.406 kb on - strand, within yecD at 682.406 kb on - strand, within yecD at 682.406 kb on - strand, within yecD at 682.406 kb on - strand, within yecD at 682.406 kb on - strand, within yecD at 682.406 kb on - strand, within yecD at 682.406 kb on - strand, within yecD at 682.406 kb on - strand, within yecD at 682.406 kb on - strand, within yecD at 682.529 kb on + strand, within yecD at 682.530 kb on - strand, within yecD at 682.530 kb on - strand, within yecD at 682.530 kb on - strand, within yecD at 682.538 kb on + strand, within yecD at 682.539 kb on - strand at 682.539 kb on - strand at 682.591 kb on + strand at 682.591 kb on + strand at 682.592 kb on - strand at 682.628 kb on - strand at 682.706 kb on + strand, within yecE at 682.707 kb on - strand, within yecE at 682.812 kb on + strand, within yecE at 682.812 kb on + strand, within yecE at 682.967 kb on - strand, within yecE at 682.970 kb on + strand, within yecE at 682.971 kb on - strand, within yecE at 682.971 kb on - strand, within yecE at 682.971 kb on - strand, within yecE at 682.971 kb on - strand, within yecE at 683.029 kb on + strand, within yecE at 683.140 kb on + strand, within yecE at 683.160 kb on + strand, within yecE at 683.161 kb on - strand, within yecE at 683.206 kb on + strand, within yecE at 683.207 kb on - strand, within yecE at 683.207 kb on - strand, within yecE at 683.289 kb on + strand, within yecE at 683.290 kb on - strand, within yecE at 683.290 kb on - strand, within yecE at 683.293 kb on + strand, within yecE at 683.293 kb on + strand, within yecE at 683.294 kb on - strand, within yecE at 683.325 kb on + strand, within yecE at 683.325 kb on + strand, within yecE at 683.325 kb on + strand, within yecE at 683.326 kb on - strand, within yecE at 683.326 kb on - strand, within yecE at 683.329 kb on + strand at 683.329 kb on + strand at 683.330 kb on - strand at 683.330 kb on - strand at 683.330 kb on - strand at 683.330 kb on - strand at 683.330 kb on - strand at 683.330 kb on - strand at 683.330 kb on - strand at 683.330 kb on - strand at 683.347 kb on - strand at 683.351 kb on - strand at 683.351 kb on - strand at 683.421 kb on + strand at 683.421 kb on + strand at 683.421 kb on + strand at 683.468 kb on - strand at 683.478 kb on + strand at 683.478 kb on + strand at 683.478 kb on + strand at 683.479 kb on - strand at 683.556 kb on + strand, within yecN at 683.619 kb on + strand, within yecN at 683.620 kb on - strand, within yecN at 683.621 kb on + strand, within yecN at 683.622 kb on - strand, within yecN at 683.687 kb on + strand, within yecN at 683.687 kb on + strand, within yecN at 683.730 kb on + strand, within yecN at 683.730 kb on + strand, within yecN at 683.823 kb on + strand at 683.823 kb on + strand at 683.823 kb on + strand at 683.823 kb on + strand at 683.824 kb on - strand at 683.825 kb on + strand at 683.826 kb on - strand at 683.866 kb on + strand at 683.898 kb on + strand at 684.039 kb on + strand, within cmoA at 684.040 kb on - strand, within cmoA at 684.141 kb on + strand, within cmoA at 684.142 kb on - strand, within cmoA at 684.202 kb on - strand, within cmoA at 684.211 kb on + strand, within cmoA at 684.222 kb on + strand, within cmoA at 684.223 kb on - strand, within cmoA at 684.230 kb on + strand, within cmoA
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 681,886 + -0.7 681,887 - -0.4 681,888 + -0.0 681,923 + -0.5 681,924 - +1.0 681,926 - +0.6 681,926 - -1.5 682,040 + -0.7 682,040 + -0.5 682,040 + +0.5 682,040 + +0.5 682,040 + -1.3 682,041 - +2.1 682,041 - +0.8 682,041 - +1.6 682,090 + yecD MCAODC_19165 0.11 +2.3 682,090 + yecD MCAODC_19165 0.11 +1.4 682,090 + yecD MCAODC_19165 0.11 -1.4 682,090 + yecD MCAODC_19165 0.11 +0.3 682,112 + yecD MCAODC_19165 0.15 +0.6 682,113 - yecD MCAODC_19165 0.15 +0.8 682,126 + yecD MCAODC_19165 0.17 +1.5 682,126 + yecD MCAODC_19165 0.17 +0.9 682,126 + yecD MCAODC_19165 0.17 -1.1 682,127 - yecD MCAODC_19165 0.17 -0.5 682,127 - yecD MCAODC_19165 0.17 -0.8 682,127 - yecD MCAODC_19165 0.17 -0.8 682,127 - yecD MCAODC_19165 0.17 +0.4 682,130 + yecD MCAODC_19165 0.18 -0.2 682,325 - yecD MCAODC_19165 0.52 -0.5 682,394 + yecD MCAODC_19165 0.64 -3.4 682,394 + yecD MCAODC_19165 0.64 -1.8 682,395 - yecD MCAODC_19165 0.65 -0.1 682,395 - yecD MCAODC_19165 0.65 -1.4 682,399 + yecD MCAODC_19165 0.65 -0.2 682,399 + yecD MCAODC_19165 0.65 -4.0 682,399 + yecD MCAODC_19165 0.65 -0.9 682,400 - yecD MCAODC_19165 0.65 +2.0 682,400 - yecD MCAODC_19165 0.65 -0.6 682,405 + yecD MCAODC_19165 0.66 +0.4 682,405 + yecD MCAODC_19165 0.66 +0.9 682,405 + yecD MCAODC_19165 0.66 -0.2 682,405 + yecD MCAODC_19165 0.66 +1.8 682,405 + yecD MCAODC_19165 0.66 +0.3 682,405 + yecD MCAODC_19165 0.66 +0.8 682,405 + yecD MCAODC_19165 0.66 -0.7 682,406 - yecD MCAODC_19165 0.66 -1.4 682,406 - yecD MCAODC_19165 0.66 -0.9 682,406 - yecD MCAODC_19165 0.66 -0.6 682,406 - yecD MCAODC_19165 0.66 -2.3 682,406 - yecD MCAODC_19165 0.66 -2.3 682,406 - yecD MCAODC_19165 0.66 -0.1 682,406 - yecD MCAODC_19165 0.66 -1.1 682,406 - yecD MCAODC_19165 0.66 -3.9 682,406 - yecD MCAODC_19165 0.66 +0.0 682,406 - yecD MCAODC_19165 0.66 -1.5 682,529 + yecD MCAODC_19165 0.88 -1.3 682,530 - yecD MCAODC_19165 0.88 +0.7 682,530 - yecD MCAODC_19165 0.88 -1.5 682,530 - yecD MCAODC_19165 0.88 +1.0 682,538 + yecD MCAODC_19165 0.90 +0.8 682,539 - -0.1 682,539 - +0.7 682,591 + -0.4 682,591 + +0.4 682,592 - -1.8 682,628 - -1.4 682,706 + yecE MCAODC_19170 0.14 -0.6 682,707 - yecE MCAODC_19170 0.14 -2.1 682,812 + yecE MCAODC_19170 0.27 -1.2 682,812 + yecE MCAODC_19170 0.27 -1.3 682,967 - yecE MCAODC_19170 0.46 -0.4 682,970 + yecE MCAODC_19170 0.46 +1.3 682,971 - yecE MCAODC_19170 0.46 -1.4 682,971 - yecE MCAODC_19170 0.46 -0.0 682,971 - yecE MCAODC_19170 0.46 -0.2 682,971 - yecE MCAODC_19170 0.46 +0.4 683,029 + yecE MCAODC_19170 0.53 -1.9 683,140 + yecE MCAODC_19170 0.67 -0.2 683,160 + yecE MCAODC_19170 0.69 +1.1 683,161 - yecE MCAODC_19170 0.69 +0.0 683,206 + yecE MCAODC_19170 0.75 -1.4 683,207 - yecE MCAODC_19170 0.75 -1.6 683,207 - yecE MCAODC_19170 0.75 -1.3 683,289 + yecE MCAODC_19170 0.85 -2.8 683,290 - yecE MCAODC_19170 0.85 +1.7 683,290 - yecE MCAODC_19170 0.85 -1.4 683,293 + yecE MCAODC_19170 0.86 -0.9 683,293 + yecE MCAODC_19170 0.86 +0.2 683,294 - yecE MCAODC_19170 0.86 +1.0 683,325 + yecE MCAODC_19170 0.89 +0.0 683,325 + yecE MCAODC_19170 0.89 -1.0 683,325 + yecE MCAODC_19170 0.89 -0.2 683,326 - yecE MCAODC_19170 0.90 +2.5 683,326 - yecE MCAODC_19170 0.90 -0.5 683,329 + +3.0 683,329 + -2.8 683,330 - -0.5 683,330 - -0.7 683,330 - +1.0 683,330 - -0.1 683,330 - -0.1 683,330 - +0.1 683,330 - -0.1 683,330 - -0.0 683,347 - +0.8 683,351 - +1.1 683,351 - -0.5 683,421 + -2.4 683,421 + -0.7 683,421 + +0.5 683,468 - +0.4 683,478 + -0.1 683,478 + -0.3 683,478 + -0.2 683,479 - +1.0 683,556 + yecN MCAODC_19175 0.23 -2.0 683,619 + yecN MCAODC_19175 0.39 -1.1 683,620 - yecN MCAODC_19175 0.40 -2.0 683,621 + yecN MCAODC_19175 0.40 +0.4 683,622 - yecN MCAODC_19175 0.40 -2.1 683,687 + yecN MCAODC_19175 0.57 -1.1 683,687 + yecN MCAODC_19175 0.57 -0.2 683,730 + yecN MCAODC_19175 0.67 +0.3 683,730 + yecN MCAODC_19175 0.67 +0.1 683,823 + -2.9 683,823 + -3.0 683,823 + +0.7 683,823 + -2.3 683,824 - -0.6 683,825 + +1.2 683,826 - -2.3 683,866 + +0.6 683,898 + -1.6 684,039 + cmoA MCAODC_19180 0.19 -1.1 684,040 - cmoA MCAODC_19180 0.19 +3.1 684,141 + cmoA MCAODC_19180 0.33 -1.7 684,142 - cmoA MCAODC_19180 0.33 -0.4 684,202 - cmoA MCAODC_19180 0.41 +2.1 684,211 + cmoA MCAODC_19180 0.42 +1.4 684,222 + cmoA MCAODC_19180 0.43 -1.2 684,223 - cmoA MCAODC_19180 0.44 -1.4 684,230 + cmoA MCAODC_19180 0.44 -0.0
Or see this region's nucleotide sequence