Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt edd and zwf are separated by 234 nucleotides zwf and MCAODC_19075 are separated by 165 nucleotides MCAODC_19075 and hexR are separated by 22 nucleotides
MCAODC_19065: edd - phosphogluconate dehydratase, at 663,891 to 665,702
edd
MCAODC_19070: zwf - glucose-6-phosphate dehydrogenase, at 665,937 to 667,412
zwf
MCAODC_19075: MCAODC_19075 - hypothetical protein, at 667,578 to 667,727
_19075
MCAODC_19080: hexR - DNA-binding transcriptional regulator HexR, at 667,750 to 668,619
hexR
Position (kb)
665
666
667
668 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 665.039 kb on + strand, within edd at 665.060 kb on - strand, within edd at 665.060 kb on - strand, within edd at 665.103 kb on - strand, within edd at 665.277 kb on - strand, within edd at 665.426 kb on + strand, within edd at 665.427 kb on - strand, within edd at 665.453 kb on - strand, within edd at 665.480 kb on + strand, within edd at 665.481 kb on - strand, within edd at 665.482 kb on + strand, within edd at 665.483 kb on - strand, within edd at 665.573 kb on + strand at 665.623 kb on - strand at 665.784 kb on - strand at 665.936 kb on - strand at 666.222 kb on - strand, within zwf at 666.361 kb on + strand, within zwf at 666.398 kb on + strand, within zwf at 666.549 kb on - strand, within zwf at 666.549 kb on - strand, within zwf at 667.322 kb on - strand at 667.425 kb on - strand at 667.430 kb on - strand at 667.446 kb on - strand at 667.446 kb on - strand at 667.446 kb on - strand at 667.446 kb on - strand at 667.458 kb on - strand at 667.458 kb on - strand at 667.458 kb on - strand at 667.458 kb on - strand at 667.513 kb on - strand at 667.542 kb on - strand at 667.596 kb on + strand, within MCAODC_19075 at 667.619 kb on + strand, within MCAODC_19075 at 667.660 kb on - strand, within MCAODC_19075 at 667.677 kb on + strand, within MCAODC_19075 at 667.677 kb on + strand, within MCAODC_19075 at 667.677 kb on + strand, within MCAODC_19075 at 667.677 kb on + strand, within MCAODC_19075 at 667.677 kb on + strand, within MCAODC_19075 at 667.987 kb on + strand, within hexR at 667.988 kb on - strand, within hexR at 668.042 kb on + strand, within hexR at 668.193 kb on + strand, within hexR at 668.193 kb on + strand, within hexR at 668.193 kb on + strand, within hexR at 668.235 kb on + strand, within hexR at 668.235 kb on + strand, within hexR at 668.358 kb on + strand, within hexR at 668.359 kb on - strand, within hexR
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 665,039 + edd MCAODC_19065 0.63 -0.3 665,060 - edd MCAODC_19065 0.65 +0.2 665,060 - edd MCAODC_19065 0.65 -1.2 665,103 - edd MCAODC_19065 0.67 -1.8 665,277 - edd MCAODC_19065 0.76 -0.2 665,426 + edd MCAODC_19065 0.85 -1.7 665,427 - edd MCAODC_19065 0.85 +0.0 665,453 - edd MCAODC_19065 0.86 -2.9 665,480 + edd MCAODC_19065 0.88 -1.8 665,481 - edd MCAODC_19065 0.88 -0.3 665,482 + edd MCAODC_19065 0.88 -0.3 665,483 - edd MCAODC_19065 0.88 -2.1 665,573 + +0.5 665,623 - -2.5 665,784 - +0.2 665,936 - -1.4 666,222 - zwf MCAODC_19070 0.19 -2.1 666,361 + zwf MCAODC_19070 0.29 -3.2 666,398 + zwf MCAODC_19070 0.31 +1.1 666,549 - zwf MCAODC_19070 0.41 -0.2 666,549 - zwf MCAODC_19070 0.41 +2.0 667,322 - +0.7 667,425 - -0.0 667,430 - -2.0 667,446 - +0.6 667,446 - +0.6 667,446 - +1.1 667,446 - +0.4 667,458 - -0.1 667,458 - -3.9 667,458 - -0.7 667,458 - -2.3 667,513 - -0.2 667,542 - +0.5 667,596 + MCAODC_19075 0.12 -1.1 667,619 + MCAODC_19075 0.27 -2.4 667,660 - MCAODC_19075 0.55 -1.4 667,677 + MCAODC_19075 0.66 +1.3 667,677 + MCAODC_19075 0.66 -0.2 667,677 + MCAODC_19075 0.66 +0.3 667,677 + MCAODC_19075 0.66 +0.1 667,677 + MCAODC_19075 0.66 +0.3 667,987 + hexR MCAODC_19080 0.27 +1.9 667,988 - hexR MCAODC_19080 0.27 -1.2 668,042 + hexR MCAODC_19080 0.34 -2.1 668,193 + hexR MCAODC_19080 0.51 -0.3 668,193 + hexR MCAODC_19080 0.51 -1.5 668,193 + hexR MCAODC_19080 0.51 +1.6 668,235 + hexR MCAODC_19080 0.56 -0.4 668,235 + hexR MCAODC_19080 0.56 +1.3 668,358 + hexR MCAODC_19080 0.70 +0.3 668,359 - hexR MCAODC_19080 0.70 -1.4
Or see this region's nucleotide sequence