Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purT and kdgA are separated by 55 nucleotides kdgA and edd are separated by 36 nucleotides edd and zwf are separated by 234 nucleotides
MCAODC_19055: purT - formate-dependent phosphoribosylglycinamide formyltransferase, at 661,979 to 663,157
purT
MCAODC_19060: kdgA - bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase, at 663,213 to 663,854
kdgA
MCAODC_19065: edd - phosphogluconate dehydratase, at 663,891 to 665,702
edd
MCAODC_19070: zwf - glucose-6-phosphate dehydrogenase, at 665,937 to 667,412
zwf
Position (kb) 663
664
665
666 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 663.141 kb on + strand at 663.147 kb on + strand at 663.214 kb on + strand at 663.222 kb on - strand at 663.231 kb on + strand at 663.252 kb on - strand at 663.349 kb on + strand, within kdgA at 663.531 kb on - strand, within kdgA at 663.892 kb on + strand at 663.903 kb on - strand at 664.114 kb on + strand, within edd at 664.129 kb on - strand, within edd at 664.239 kb on - strand, within edd at 664.560 kb on - strand, within edd at 664.693 kb on + strand, within edd at 664.714 kb on - strand, within edd at 664.907 kb on + strand, within edd at 665.039 kb on + strand, within edd at 665.060 kb on - strand, within edd at 665.060 kb on - strand, within edd at 665.103 kb on - strand, within edd at 665.277 kb on - strand, within edd at 665.426 kb on + strand, within edd at 665.427 kb on - strand, within edd at 665.453 kb on - strand, within edd at 665.480 kb on + strand, within edd at 665.481 kb on - strand, within edd at 665.482 kb on + strand, within edd at 665.483 kb on - strand, within edd at 665.573 kb on + strand at 665.623 kb on - strand at 665.784 kb on - strand at 665.936 kb on - strand at 666.222 kb on - strand, within zwf at 666.361 kb on + strand, within zwf at 666.398 kb on + strand, within zwf at 666.549 kb on - strand, within zwf at 666.549 kb on - strand, within zwf
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 663,141 + -2.3 663,147 + -0.8 663,214 + -1.2 663,222 - -0.1 663,231 + +0.1 663,252 - -0.9 663,349 + kdgA MCAODC_19060 0.21 -0.1 663,531 - kdgA MCAODC_19060 0.50 +0.1 663,892 + +0.8 663,903 - -2.8 664,114 + edd MCAODC_19065 0.12 -0.9 664,129 - edd MCAODC_19065 0.13 +0.7 664,239 - edd MCAODC_19065 0.19 +0.6 664,560 - edd MCAODC_19065 0.37 -0.7 664,693 + edd MCAODC_19065 0.44 -0.6 664,714 - edd MCAODC_19065 0.45 -2.2 664,907 + edd MCAODC_19065 0.56 -1.9 665,039 + edd MCAODC_19065 0.63 -0.3 665,060 - edd MCAODC_19065 0.65 +0.2 665,060 - edd MCAODC_19065 0.65 -1.2 665,103 - edd MCAODC_19065 0.67 -1.8 665,277 - edd MCAODC_19065 0.76 -0.2 665,426 + edd MCAODC_19065 0.85 -1.7 665,427 - edd MCAODC_19065 0.85 +0.0 665,453 - edd MCAODC_19065 0.86 -2.9 665,480 + edd MCAODC_19065 0.88 -1.8 665,481 - edd MCAODC_19065 0.88 -0.3 665,482 + edd MCAODC_19065 0.88 -0.3 665,483 - edd MCAODC_19065 0.88 -2.1 665,573 + +0.5 665,623 - -2.5 665,784 - +0.2 665,936 - -1.4 666,222 - zwf MCAODC_19070 0.19 -2.1 666,361 + zwf MCAODC_19070 0.29 -3.2 666,398 + zwf MCAODC_19070 0.31 +1.1 666,549 - zwf MCAODC_19070 0.41 -0.2 666,549 - zwf MCAODC_19070 0.41 +2.0
Or see this region's nucleotide sequence