Strain Fitness in Escherichia coli ECRC101 around MCAODC_19025

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyobB and exoX are separated by 23 nucleotidesexoX and ptrB overlap by 4 nucleotidesptrB and yebE are separated by 208 nucleotidesyebE and MCAODC_19035 are separated by 59 nucleotides MCAODC_19015: yobB - Uncharacterized protein YobB, at 656,538 to 657,194 yobB MCAODC_19020: exoX - exodeoxyribonuclease X, at 657,218 to 657,880 exoX MCAODC_19025: ptrB - oligopeptidase B, at 657,877 to 659,937 ptrB MCAODC_19030: yebE - Inner membrane protein YebE, at 660,146 to 660,805 yebE MCAODC_19035: MCAODC_19035 - hypothetical protein, at 660,865 to 660,975 _19035 Position (kb) 657 658 659 660Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 656.936 kb on + strand, within yobBat 656.937 kb on - strand, within yobBat 656.942 kb on + strand, within yobBat 657.134 kb on - strandat 657.177 kb on - strandat 657.187 kb on + strandat 657.187 kb on + strandat 657.229 kb on + strandat 657.428 kb on + strand, within exoXat 657.428 kb on + strand, within exoXat 657.429 kb on - strand, within exoXat 657.431 kb on + strand, within exoXat 657.446 kb on + strand, within exoXat 657.458 kb on - strand, within exoXat 657.551 kb on + strand, within exoXat 657.559 kb on + strand, within exoXat 657.569 kb on + strand, within exoXat 657.571 kb on + strand, within exoXat 657.571 kb on + strand, within exoXat 657.629 kb on + strand, within exoXat 657.629 kb on + strand, within exoXat 657.629 kb on + strand, within exoXat 657.638 kb on + strand, within exoXat 657.639 kb on - strand, within exoXat 657.639 kb on - strand, within exoXat 657.667 kb on + strand, within exoXat 657.753 kb on - strand, within exoXat 657.753 kb on - strand, within exoXat 657.797 kb on + strand, within exoXat 657.878 kb on + strandat 657.961 kb on - strandat 658.012 kb on + strandat 658.013 kb on - strandat 658.093 kb on + strand, within ptrBat 658.121 kb on + strand, within ptrBat 658.167 kb on - strand, within ptrBat 658.167 kb on - strand, within ptrBat 658.172 kb on + strand, within ptrBat 658.173 kb on - strand, within ptrBat 658.173 kb on - strand, within ptrBat 658.252 kb on + strand, within ptrBat 658.294 kb on + strand, within ptrBat 658.429 kb on - strand, within ptrBat 658.508 kb on + strand, within ptrBat 658.526 kb on - strand, within ptrBat 658.563 kb on + strand, within ptrBat 658.563 kb on + strand, within ptrBat 658.584 kb on - strand, within ptrBat 658.589 kb on + strand, within ptrBat 658.590 kb on - strand, within ptrBat 658.860 kb on - strand, within ptrBat 658.860 kb on - strand, within ptrBat 659.051 kb on + strand, within ptrBat 659.053 kb on + strand, within ptrBat 659.227 kb on + strand, within ptrBat 659.227 kb on + strand, within ptrBat 659.227 kb on + strand, within ptrBat 659.228 kb on - strand, within ptrBat 659.228 kb on - strand, within ptrBat 659.231 kb on + strand, within ptrBat 659.250 kb on + strand, within ptrBat 659.251 kb on - strand, within ptrBat 659.268 kb on - strand, within ptrBat 659.271 kb on + strand, within ptrBat 659.375 kb on + strand, within ptrBat 659.376 kb on - strand, within ptrBat 659.379 kb on - strand, within ptrBat 659.481 kb on - strand, within ptrBat 659.568 kb on + strand, within ptrBat 659.569 kb on - strand, within ptrBat 659.645 kb on + strand, within ptrBat 659.649 kb on + strand, within ptrBat 659.649 kb on + strand, within ptrBat 659.650 kb on - strand, within ptrBat 659.676 kb on + strand, within ptrBat 659.752 kb on + strandat 659.805 kb on + strandat 659.941 kb on - strandat 659.966 kb on + strandat 659.967 kb on - strandat 659.967 kb on - strandat 659.967 kb on - strandat 659.968 kb on + strandat 659.969 kb on - strandat 659.969 kb on - strandat 659.969 kb on - strandat 659.969 kb on - strandat 660.244 kb on - strand, within yebEat 660.269 kb on - strand, within yebEat 660.294 kb on + strand, within yebEat 660.451 kb on + strand, within yebEat 660.451 kb on + strand, within yebEat 660.521 kb on - strand, within yebEat 660.577 kb on - strand, within yebEat 660.577 kb on - strand, within yebEat 660.639 kb on + strand, within yebEat 660.668 kb on - strand, within yebEat 660.668 kb on - strand, within yebEat 660.806 kb on - strandat 660.806 kb on - strandat 660.806 kb on - strandat 660.806 kb on - strandat 660.806 kb on - strandat 660.838 kb on + strandat 660.839 kb on - strandat 660.868 kb on + strandat 660.869 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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656,936 + yobB MCAODC_19015 0.61 +1.5
656,937 - yobB MCAODC_19015 0.61 -0.7
656,942 + yobB MCAODC_19015 0.61 -1.1
657,134 - -0.7
657,177 - +0.2
657,187 + +0.4
657,187 + -0.2
657,229 + +0.7
657,428 + exoX MCAODC_19020 0.32 -0.2
657,428 + exoX MCAODC_19020 0.32 +0.6
657,429 - exoX MCAODC_19020 0.32 -1.9
657,431 + exoX MCAODC_19020 0.32 -1.7
657,446 + exoX MCAODC_19020 0.34 -3.3
657,458 - exoX MCAODC_19020 0.36 -1.0
657,551 + exoX MCAODC_19020 0.50 -2.0
657,559 + exoX MCAODC_19020 0.51 -0.0
657,569 + exoX MCAODC_19020 0.53 -0.5
657,571 + exoX MCAODC_19020 0.53 +0.8
657,571 + exoX MCAODC_19020 0.53 -0.6
657,629 + exoX MCAODC_19020 0.62 -0.4
657,629 + exoX MCAODC_19020 0.62 -1.6
657,629 + exoX MCAODC_19020 0.62 +0.7
657,638 + exoX MCAODC_19020 0.63 -0.9
657,639 - exoX MCAODC_19020 0.63 -0.2
657,639 - exoX MCAODC_19020 0.63 -2.6
657,667 + exoX MCAODC_19020 0.68 -0.3
657,753 - exoX MCAODC_19020 0.81 +1.4
657,753 - exoX MCAODC_19020 0.81 -0.9
657,797 + exoX MCAODC_19020 0.87 -3.1
657,878 + -1.6
657,961 - +0.2
658,012 + +0.5
658,013 - -3.0
658,093 + ptrB MCAODC_19025 0.10 +1.4
658,121 + ptrB MCAODC_19025 0.12 +1.6
658,167 - ptrB MCAODC_19025 0.14 +1.0
658,167 - ptrB MCAODC_19025 0.14 +0.5
658,172 + ptrB MCAODC_19025 0.14 +1.3
658,173 - ptrB MCAODC_19025 0.14 +0.7
658,173 - ptrB MCAODC_19025 0.14 -0.2
658,252 + ptrB MCAODC_19025 0.18 -0.9
658,294 + ptrB MCAODC_19025 0.20 -1.2
658,429 - ptrB MCAODC_19025 0.27 -1.2
658,508 + ptrB MCAODC_19025 0.31 -2.1
658,526 - ptrB MCAODC_19025 0.31 +1.4
658,563 + ptrB MCAODC_19025 0.33 +0.1
658,563 + ptrB MCAODC_19025 0.33 -0.5
658,584 - ptrB MCAODC_19025 0.34 -1.1
658,589 + ptrB MCAODC_19025 0.35 -0.4
658,590 - ptrB MCAODC_19025 0.35 -0.1
658,860 - ptrB MCAODC_19025 0.48 +1.0
658,860 - ptrB MCAODC_19025 0.48 -3.5
659,051 + ptrB MCAODC_19025 0.57 -1.2
659,053 + ptrB MCAODC_19025 0.57 -0.7
659,227 + ptrB MCAODC_19025 0.66 -3.0
659,227 + ptrB MCAODC_19025 0.66 +0.8
659,227 + ptrB MCAODC_19025 0.66 +2.3
659,228 - ptrB MCAODC_19025 0.66 -0.9
659,228 - ptrB MCAODC_19025 0.66 -0.2
659,231 + ptrB MCAODC_19025 0.66 +1.6
659,250 + ptrB MCAODC_19025 0.67 -0.7
659,251 - ptrB MCAODC_19025 0.67 -0.1
659,268 - ptrB MCAODC_19025 0.67 -2.4
659,271 + ptrB MCAODC_19025 0.68 +2.6
659,375 + ptrB MCAODC_19025 0.73 -1.5
659,376 - ptrB MCAODC_19025 0.73 -0.1
659,379 - ptrB MCAODC_19025 0.73 -0.2
659,481 - ptrB MCAODC_19025 0.78 +0.5
659,568 + ptrB MCAODC_19025 0.82 +0.8
659,569 - ptrB MCAODC_19025 0.82 +0.9
659,645 + ptrB MCAODC_19025 0.86 +0.0
659,649 + ptrB MCAODC_19025 0.86 -0.4
659,649 + ptrB MCAODC_19025 0.86 -0.5
659,650 - ptrB MCAODC_19025 0.86 +0.2
659,676 + ptrB MCAODC_19025 0.87 +1.1
659,752 + +0.2
659,805 + -0.1
659,941 - +0.0
659,966 + -0.5
659,967 - -0.5
659,967 - +1.7
659,967 - -0.3
659,968 + +0.4
659,969 - -0.0
659,969 - -1.6
659,969 - +0.5
659,969 - -0.1
660,244 - yebE MCAODC_19030 0.15 -2.1
660,269 - yebE MCAODC_19030 0.19 +0.1
660,294 + yebE MCAODC_19030 0.22 +0.3
660,451 + yebE MCAODC_19030 0.46 -1.7
660,451 + yebE MCAODC_19030 0.46 +0.1
660,521 - yebE MCAODC_19030 0.57 -0.0
660,577 - yebE MCAODC_19030 0.65 -1.0
660,577 - yebE MCAODC_19030 0.65 -0.9
660,639 + yebE MCAODC_19030 0.75 -0.7
660,668 - yebE MCAODC_19030 0.79 +0.8
660,668 - yebE MCAODC_19030 0.79 -1.0
660,806 - -0.4
660,806 - -1.9
660,806 - -0.9
660,806 - -1.1
660,806 - -1.1
660,838 + -0.7
660,839 - -0.6
660,868 + -0.8
660,869 - +1.2

Or see this region's nucleotide sequence