Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt copD and yobA are separated by 3 nucleotides yobA and holE are separated by 138 nucleotides holE and yobB are separated by 101 nucleotides yobB and exoX are separated by 23 nucleotides exoX and ptrB overlap by 4 nucleotides
MCAODC_19000: copD - copper homeostasis membrane protein CopD, at 654,817 to 655,689
copD
MCAODC_19005: yobA - CopC domain-containing protein YobA, at 655,693 to 656,067
yobA
MCAODC_19010: holE - DNA polymerase III subunit theta, at 656,206 to 656,436
holE
MCAODC_19015: yobB - Uncharacterized protein YobB, at 656,538 to 657,194
yobB
MCAODC_19020: exoX - exodeoxyribonuclease X, at 657,218 to 657,880
exoX
MCAODC_19025: ptrB - oligopeptidase B, at 657,877 to 659,937
ptrB
Position (kb) 656
657
658 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 655.553 kb on - strand, within copD at 655.613 kb on + strand at 655.613 kb on + strand at 655.613 kb on + strand at 655.614 kb on - strand at 655.694 kb on + strand at 655.767 kb on - strand, within yobA at 655.967 kb on + strand, within yobA at 655.979 kb on - strand, within yobA at 656.070 kb on + strand at 656.070 kb on + strand at 656.070 kb on + strand at 656.071 kb on - strand at 656.071 kb on - strand at 656.075 kb on - strand at 656.090 kb on - strand at 656.170 kb on - strand at 656.201 kb on + strand at 656.287 kb on - strand, within holE at 656.297 kb on - strand, within holE at 656.297 kb on - strand, within holE at 656.302 kb on - strand, within holE at 656.302 kb on - strand, within holE at 656.302 kb on - strand, within holE at 656.373 kb on + strand, within holE at 656.374 kb on - strand, within holE at 656.452 kb on - strand at 656.462 kb on - strand at 656.465 kb on + strand at 656.466 kb on - strand at 656.475 kb on + strand at 656.487 kb on - strand at 656.568 kb on + strand at 656.579 kb on + strand at 656.602 kb on + strand at 656.799 kb on + strand, within yobB at 656.801 kb on + strand, within yobB at 656.802 kb on - strand, within yobB at 656.863 kb on - strand, within yobB at 656.936 kb on + strand, within yobB at 656.937 kb on - strand, within yobB at 656.942 kb on + strand, within yobB at 657.134 kb on - strand at 657.177 kb on - strand at 657.187 kb on + strand at 657.187 kb on + strand at 657.229 kb on + strand at 657.428 kb on + strand, within exoX at 657.428 kb on + strand, within exoX at 657.429 kb on - strand, within exoX at 657.431 kb on + strand, within exoX at 657.446 kb on + strand, within exoX at 657.458 kb on - strand, within exoX at 657.551 kb on + strand, within exoX at 657.559 kb on + strand, within exoX at 657.569 kb on + strand, within exoX at 657.571 kb on + strand, within exoX at 657.571 kb on + strand, within exoX at 657.629 kb on + strand, within exoX at 657.629 kb on + strand, within exoX at 657.629 kb on + strand, within exoX at 657.638 kb on + strand, within exoX at 657.639 kb on - strand, within exoX at 657.639 kb on - strand, within exoX at 657.667 kb on + strand, within exoX at 657.753 kb on - strand, within exoX at 657.753 kb on - strand, within exoX at 657.797 kb on + strand, within exoX at 657.878 kb on + strand at 657.961 kb on - strand at 658.012 kb on + strand at 658.013 kb on - strand at 658.093 kb on + strand, within ptrB at 658.121 kb on + strand, within ptrB at 658.167 kb on - strand, within ptrB at 658.167 kb on - strand, within ptrB at 658.172 kb on + strand, within ptrB at 658.173 kb on - strand, within ptrB at 658.173 kb on - strand, within ptrB
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 655,553 - copD MCAODC_19000 0.84 -1.3 655,613 + -1.6 655,613 + -1.8 655,613 + -0.0 655,614 - -0.4 655,694 + -0.2 655,767 - yobA MCAODC_19005 0.20 -0.1 655,967 + yobA MCAODC_19005 0.73 -0.0 655,979 - yobA MCAODC_19005 0.76 -1.2 656,070 + -1.8 656,070 + -0.5 656,070 + +0.3 656,071 - -0.1 656,071 - +0.6 656,075 - -0.5 656,090 - +0.8 656,170 - +0.9 656,201 + -0.0 656,287 - holE MCAODC_19010 0.35 +1.8 656,297 - holE MCAODC_19010 0.39 -0.0 656,297 - holE MCAODC_19010 0.39 +0.3 656,302 - holE MCAODC_19010 0.42 -1.9 656,302 - holE MCAODC_19010 0.42 -2.6 656,302 - holE MCAODC_19010 0.42 -2.0 656,373 + holE MCAODC_19010 0.72 +2.0 656,374 - holE MCAODC_19010 0.73 +0.5 656,452 - +1.4 656,462 - -0.3 656,465 + -1.3 656,466 - +0.5 656,475 + +1.5 656,487 - +0.1 656,568 + +0.1 656,579 + +0.4 656,602 + +2.2 656,799 + yobB MCAODC_19015 0.40 -0.1 656,801 + yobB MCAODC_19015 0.40 -0.9 656,802 - yobB MCAODC_19015 0.40 +0.6 656,863 - yobB MCAODC_19015 0.49 +0.2 656,936 + yobB MCAODC_19015 0.61 +1.5 656,937 - yobB MCAODC_19015 0.61 -0.7 656,942 + yobB MCAODC_19015 0.61 -1.1 657,134 - -0.7 657,177 - +0.2 657,187 + +0.4 657,187 + -0.2 657,229 + +0.7 657,428 + exoX MCAODC_19020 0.32 -0.2 657,428 + exoX MCAODC_19020 0.32 +0.6 657,429 - exoX MCAODC_19020 0.32 -1.9 657,431 + exoX MCAODC_19020 0.32 -1.7 657,446 + exoX MCAODC_19020 0.34 -3.3 657,458 - exoX MCAODC_19020 0.36 -1.0 657,551 + exoX MCAODC_19020 0.50 -2.0 657,559 + exoX MCAODC_19020 0.51 -0.0 657,569 + exoX MCAODC_19020 0.53 -0.5 657,571 + exoX MCAODC_19020 0.53 +0.8 657,571 + exoX MCAODC_19020 0.53 -0.6 657,629 + exoX MCAODC_19020 0.62 -0.4 657,629 + exoX MCAODC_19020 0.62 -1.6 657,629 + exoX MCAODC_19020 0.62 +0.7 657,638 + exoX MCAODC_19020 0.63 -0.9 657,639 - exoX MCAODC_19020 0.63 -0.2 657,639 - exoX MCAODC_19020 0.63 -2.6 657,667 + exoX MCAODC_19020 0.68 -0.3 657,753 - exoX MCAODC_19020 0.81 +1.4 657,753 - exoX MCAODC_19020 0.81 -0.9 657,797 + exoX MCAODC_19020 0.87 -3.1 657,878 + -1.6 657,961 - +0.2 658,012 + +0.5 658,013 - -3.0 658,093 + ptrB MCAODC_19025 0.10 +1.4 658,121 + ptrB MCAODC_19025 0.12 +1.6 658,167 - ptrB MCAODC_19025 0.14 +1.0 658,167 - ptrB MCAODC_19025 0.14 +0.5 658,172 + ptrB MCAODC_19025 0.14 +1.3 658,173 - ptrB MCAODC_19025 0.14 +0.7 658,173 - ptrB MCAODC_19025 0.14 -0.2
Or see this region's nucleotide sequence