Strain Fitness in Escherichia coli ECRC101 around MCAODC_19005

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyebY and copD are separated by 12 nucleotidescopD and yobA are separated by 3 nucleotidesyobA and holE are separated by 138 nucleotidesholE and yobB are separated by 101 nucleotides MCAODC_18995: yebY - Uncharacterized protein YebY, at 654,463 to 654,804 yebY MCAODC_19000: copD - copper homeostasis membrane protein CopD, at 654,817 to 655,689 copD MCAODC_19005: yobA - CopC domain-containing protein YobA, at 655,693 to 656,067 yobA MCAODC_19010: holE - DNA polymerase III subunit theta, at 656,206 to 656,436 holE MCAODC_19015: yobB - Uncharacterized protein YobB, at 656,538 to 657,194 yobB Position (kb) 655 656 657Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 654.706 kb on - strand, within yebYat 654.944 kb on - strand, within copDat 654.953 kb on + strand, within copDat 654.954 kb on - strand, within copDat 654.982 kb on - strand, within copDat 654.982 kb on - strand, within copDat 654.982 kb on - strand, within copDat 655.010 kb on - strand, within copDat 655.025 kb on + strand, within copDat 655.033 kb on - strand, within copDat 655.068 kb on + strand, within copDat 655.141 kb on - strand, within copDat 655.176 kb on + strand, within copDat 655.176 kb on + strand, within copDat 655.177 kb on - strand, within copDat 655.177 kb on - strand, within copDat 655.177 kb on - strand, within copDat 655.195 kb on + strand, within copDat 655.242 kb on + strand, within copDat 655.242 kb on + strand, within copDat 655.330 kb on - strand, within copDat 655.330 kb on - strand, within copDat 655.330 kb on - strand, within copDat 655.462 kb on + strand, within copDat 655.463 kb on - strand, within copDat 655.553 kb on - strand, within copDat 655.613 kb on + strandat 655.613 kb on + strandat 655.613 kb on + strandat 655.614 kb on - strandat 655.694 kb on + strandat 655.767 kb on - strand, within yobAat 655.967 kb on + strand, within yobAat 655.979 kb on - strand, within yobAat 656.070 kb on + strandat 656.070 kb on + strandat 656.070 kb on + strandat 656.071 kb on - strandat 656.071 kb on - strandat 656.075 kb on - strandat 656.090 kb on - strandat 656.170 kb on - strandat 656.201 kb on + strandat 656.287 kb on - strand, within holEat 656.297 kb on - strand, within holEat 656.297 kb on - strand, within holEat 656.302 kb on - strand, within holEat 656.302 kb on - strand, within holEat 656.302 kb on - strand, within holEat 656.373 kb on + strand, within holEat 656.374 kb on - strand, within holEat 656.452 kb on - strandat 656.462 kb on - strandat 656.465 kb on + strandat 656.466 kb on - strandat 656.475 kb on + strandat 656.487 kb on - strandat 656.568 kb on + strandat 656.579 kb on + strandat 656.602 kb on + strandat 656.799 kb on + strand, within yobBat 656.801 kb on + strand, within yobBat 656.802 kb on - strand, within yobBat 656.863 kb on - strand, within yobBat 656.936 kb on + strand, within yobBat 656.937 kb on - strand, within yobBat 656.942 kb on + strand, within yobB

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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654,706 - yebY MCAODC_18995 0.71 -0.7
654,944 - copD MCAODC_19000 0.15 -3.6
654,953 + copD MCAODC_19000 0.16 +3.2
654,954 - copD MCAODC_19000 0.16 +0.1
654,982 - copD MCAODC_19000 0.19 +0.8
654,982 - copD MCAODC_19000 0.19 -2.1
654,982 - copD MCAODC_19000 0.19 -0.8
655,010 - copD MCAODC_19000 0.22 +0.2
655,025 + copD MCAODC_19000 0.24 -1.8
655,033 - copD MCAODC_19000 0.25 +0.9
655,068 + copD MCAODC_19000 0.29 +2.4
655,141 - copD MCAODC_19000 0.37 +0.2
655,176 + copD MCAODC_19000 0.41 +0.4
655,176 + copD MCAODC_19000 0.41 -2.2
655,177 - copD MCAODC_19000 0.41 -0.5
655,177 - copD MCAODC_19000 0.41 -0.2
655,177 - copD MCAODC_19000 0.41 -0.3
655,195 + copD MCAODC_19000 0.43 +1.7
655,242 + copD MCAODC_19000 0.49 +0.1
655,242 + copD MCAODC_19000 0.49 +1.7
655,330 - copD MCAODC_19000 0.59 -1.6
655,330 - copD MCAODC_19000 0.59 +0.5
655,330 - copD MCAODC_19000 0.59 +1.8
655,462 + copD MCAODC_19000 0.74 -1.1
655,463 - copD MCAODC_19000 0.74 -0.2
655,553 - copD MCAODC_19000 0.84 -1.3
655,613 + -1.6
655,613 + -1.8
655,613 + -0.0
655,614 - -0.4
655,694 + -0.2
655,767 - yobA MCAODC_19005 0.20 -0.1
655,967 + yobA MCAODC_19005 0.73 -0.0
655,979 - yobA MCAODC_19005 0.76 -1.2
656,070 + -1.8
656,070 + -0.5
656,070 + +0.3
656,071 - -0.1
656,071 - +0.6
656,075 - -0.5
656,090 - +0.8
656,170 - +0.9
656,201 + -0.0
656,287 - holE MCAODC_19010 0.35 +1.8
656,297 - holE MCAODC_19010 0.39 -0.0
656,297 - holE MCAODC_19010 0.39 +0.3
656,302 - holE MCAODC_19010 0.42 -1.9
656,302 - holE MCAODC_19010 0.42 -2.6
656,302 - holE MCAODC_19010 0.42 -2.0
656,373 + holE MCAODC_19010 0.72 +2.0
656,374 - holE MCAODC_19010 0.73 +0.5
656,452 - +1.4
656,462 - -0.3
656,465 + -1.3
656,466 - +0.5
656,475 + +1.5
656,487 - +0.1
656,568 + +0.1
656,579 + +0.4
656,602 + +2.2
656,799 + yobB MCAODC_19015 0.40 -0.1
656,801 + yobB MCAODC_19015 0.40 -0.9
656,802 - yobB MCAODC_19015 0.40 +0.6
656,863 - yobB MCAODC_19015 0.49 +0.2
656,936 + yobB MCAODC_19015 0.61 +1.5
656,937 - yobB MCAODC_19015 0.61 -0.7
656,942 + yobB MCAODC_19015 0.61 -1.1

Or see this region's nucleotide sequence