Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pphA and yebY are separated by 395 nucleotides yebY and copD are separated by 12 nucleotides copD and yobA are separated by 3 nucleotides yobA and holE are separated by 138 nucleotides holE and yobB are separated by 101 nucleotides
MCAODC_18980: pphA - protein-serine/threonine phosphatase, at 653,411 to 654,067
pphA
MCAODC_18995: yebY - Uncharacterized protein YebY, at 654,463 to 654,804
yebY
MCAODC_19000: copD - copper homeostasis membrane protein CopD, at 654,817 to 655,689
copD
MCAODC_19005: yobA - CopC domain-containing protein YobA, at 655,693 to 656,067
yobA
MCAODC_19010: holE - DNA polymerase III subunit theta, at 656,206 to 656,436
holE
MCAODC_19015: yobB - Uncharacterized protein YobB, at 656,538 to 657,194
yobB
Position (kb) 654
655
656 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 653.927 kb on + strand, within pphA at 653.993 kb on + strand, within pphA at 653.994 kb on - strand, within pphA at 653.994 kb on - strand, within pphA at 654.014 kb on - strand at 654.014 kb on - strand at 654.014 kb on - strand at 654.202 kb on - strand at 654.227 kb on + strand at 654.283 kb on - strand at 654.293 kb on + strand at 654.293 kb on + strand at 654.293 kb on + strand at 654.294 kb on - strand at 654.294 kb on - strand at 654.315 kb on + strand at 654.315 kb on + strand at 654.316 kb on - strand at 654.360 kb on + strand at 654.384 kb on + strand at 654.384 kb on + strand at 654.384 kb on + strand at 654.385 kb on - strand at 654.465 kb on - strand at 654.488 kb on - strand at 654.641 kb on + strand, within yebY at 654.641 kb on + strand, within yebY at 654.641 kb on + strand, within yebY at 654.706 kb on - strand, within yebY at 654.944 kb on - strand, within copD at 654.953 kb on + strand, within copD at 654.954 kb on - strand, within copD at 654.982 kb on - strand, within copD at 654.982 kb on - strand, within copD at 654.982 kb on - strand, within copD at 655.010 kb on - strand, within copD at 655.025 kb on + strand, within copD at 655.033 kb on - strand, within copD at 655.068 kb on + strand, within copD at 655.141 kb on - strand, within copD at 655.176 kb on + strand, within copD at 655.176 kb on + strand, within copD at 655.177 kb on - strand, within copD at 655.177 kb on - strand, within copD at 655.177 kb on - strand, within copD at 655.195 kb on + strand, within copD at 655.242 kb on + strand, within copD at 655.242 kb on + strand, within copD at 655.330 kb on - strand, within copD at 655.330 kb on - strand, within copD at 655.330 kb on - strand, within copD at 655.462 kb on + strand, within copD at 655.463 kb on - strand, within copD at 655.553 kb on - strand, within copD at 655.613 kb on + strand at 655.613 kb on + strand at 655.613 kb on + strand at 655.614 kb on - strand at 655.694 kb on + strand at 655.767 kb on - strand, within yobA at 655.967 kb on + strand, within yobA at 655.979 kb on - strand, within yobA at 656.070 kb on + strand at 656.070 kb on + strand at 656.070 kb on + strand at 656.071 kb on - strand at 656.071 kb on - strand at 656.075 kb on - strand at 656.090 kb on - strand at 656.170 kb on - strand at 656.201 kb on + strand at 656.287 kb on - strand, within holE at 656.297 kb on - strand, within holE at 656.297 kb on - strand, within holE at 656.302 kb on - strand, within holE at 656.302 kb on - strand, within holE at 656.302 kb on - strand, within holE at 656.373 kb on + strand, within holE at 656.374 kb on - strand, within holE at 656.452 kb on - strand at 656.462 kb on - strand at 656.465 kb on + strand at 656.466 kb on - strand at 656.475 kb on + strand at 656.487 kb on - strand at 656.568 kb on + strand at 656.579 kb on + strand at 656.602 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 653,927 + pphA MCAODC_18980 0.79 +1.0 653,993 + pphA MCAODC_18980 0.89 -2.6 653,994 - pphA MCAODC_18980 0.89 +1.2 653,994 - pphA MCAODC_18980 0.89 +2.5 654,014 - -2.2 654,014 - +1.6 654,014 - -1.2 654,202 - +0.7 654,227 + +0.9 654,283 - +1.4 654,293 + +0.5 654,293 + -1.8 654,293 + +0.7 654,294 - -2.3 654,294 - -0.8 654,315 + +1.0 654,315 + -1.3 654,316 - -1.1 654,360 + -0.3 654,384 + +1.3 654,384 + +2.1 654,384 + -1.5 654,385 - -1.2 654,465 - +1.5 654,488 - +0.3 654,641 + yebY MCAODC_18995 0.52 -1.1 654,641 + yebY MCAODC_18995 0.52 -0.5 654,641 + yebY MCAODC_18995 0.52 +2.1 654,706 - yebY MCAODC_18995 0.71 -0.7 654,944 - copD MCAODC_19000 0.15 -3.6 654,953 + copD MCAODC_19000 0.16 +3.2 654,954 - copD MCAODC_19000 0.16 +0.1 654,982 - copD MCAODC_19000 0.19 +0.8 654,982 - copD MCAODC_19000 0.19 -2.1 654,982 - copD MCAODC_19000 0.19 -0.8 655,010 - copD MCAODC_19000 0.22 +0.2 655,025 + copD MCAODC_19000 0.24 -1.8 655,033 - copD MCAODC_19000 0.25 +0.9 655,068 + copD MCAODC_19000 0.29 +2.4 655,141 - copD MCAODC_19000 0.37 +0.2 655,176 + copD MCAODC_19000 0.41 +0.4 655,176 + copD MCAODC_19000 0.41 -2.2 655,177 - copD MCAODC_19000 0.41 -0.5 655,177 - copD MCAODC_19000 0.41 -0.2 655,177 - copD MCAODC_19000 0.41 -0.3 655,195 + copD MCAODC_19000 0.43 +1.7 655,242 + copD MCAODC_19000 0.49 +0.1 655,242 + copD MCAODC_19000 0.49 +1.7 655,330 - copD MCAODC_19000 0.59 -1.6 655,330 - copD MCAODC_19000 0.59 +0.5 655,330 - copD MCAODC_19000 0.59 +1.8 655,462 + copD MCAODC_19000 0.74 -1.1 655,463 - copD MCAODC_19000 0.74 -0.2 655,553 - copD MCAODC_19000 0.84 -1.3 655,613 + -1.6 655,613 + -1.8 655,613 + -0.0 655,614 - -0.4 655,694 + -0.2 655,767 - yobA MCAODC_19005 0.20 -0.1 655,967 + yobA MCAODC_19005 0.73 -0.0 655,979 - yobA MCAODC_19005 0.76 -1.2 656,070 + -1.8 656,070 + -0.5 656,070 + +0.3 656,071 - -0.1 656,071 - +0.6 656,075 - -0.5 656,090 - +0.8 656,170 - +0.9 656,201 + -0.0 656,287 - holE MCAODC_19010 0.35 +1.8 656,297 - holE MCAODC_19010 0.39 -0.0 656,297 - holE MCAODC_19010 0.39 +0.3 656,302 - holE MCAODC_19010 0.42 -1.9 656,302 - holE MCAODC_19010 0.42 -2.6 656,302 - holE MCAODC_19010 0.42 -2.0 656,373 + holE MCAODC_19010 0.72 +2.0 656,374 - holE MCAODC_19010 0.73 +0.5 656,452 - +1.4 656,462 - -0.3 656,465 + -1.3 656,466 - +0.5 656,475 + +1.5 656,487 - +0.1 656,568 + +0.1 656,579 + +0.4 656,602 + +2.2
Or see this region's nucleotide sequence