Strain Fitness in Escherichia coli ECRC101 around MCAODC_18115

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydhY and fumD are separated by 455 nucleotidesfumD and pykF are separated by 556 nucleotides MCAODC_18110: ydhY - ferredoxin-like protein, at 482,831 to 483,457 ydhY MCAODC_18115: fumD - fumarate hydratase FumD, at 483,913 to 484,122 fumD MCAODC_18120: pykF - pyruvate kinase PykF, at 484,679 to 486,091 pykF Position (kb) 483 484 485Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 482.936 kb on - strand, within ydhYat 483.163 kb on - strand, within ydhYat 483.292 kb on + strand, within ydhYat 483.297 kb on - strand, within ydhYat 483.511 kb on + strandat 483.524 kb on - strandat 483.920 kb on - strandat 483.997 kb on - strand, within fumDat 484.000 kb on + strand, within fumDat 484.001 kb on - strand, within fumDat 484.021 kb on - strand, within fumDat 484.021 kb on - strand, within fumDat 484.021 kb on - strand, within fumDat 484.138 kb on - strandat 484.150 kb on - strandat 484.159 kb on + strandat 484.159 kb on + strandat 484.160 kb on - strandat 484.160 kb on - strandat 484.222 kb on + strandat 484.247 kb on + strandat 484.248 kb on - strandat 484.254 kb on - strandat 484.274 kb on + strandat 484.351 kb on + strandat 484.363 kb on - strandat 484.378 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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482,936 - ydhY MCAODC_18110 0.17 -0.7
483,163 - ydhY MCAODC_18110 0.53 -2.2
483,292 + ydhY MCAODC_18110 0.74 +0.1
483,297 - ydhY MCAODC_18110 0.74 -0.1
483,511 + -2.0
483,524 - +0.6
483,920 - -1.8
483,997 - fumD MCAODC_18115 0.40 -0.4
484,000 + fumD MCAODC_18115 0.41 +1.5
484,001 - fumD MCAODC_18115 0.42 -0.7
484,021 - fumD MCAODC_18115 0.51 +0.2
484,021 - fumD MCAODC_18115 0.51 +1.2
484,021 - fumD MCAODC_18115 0.51 -1.5
484,138 - -3.1
484,150 - -0.5
484,159 + -1.6
484,159 + +3.0
484,160 - -0.1
484,160 - +0.1
484,222 + +0.4
484,247 + +0.5
484,248 - -2.6
484,254 - +0.4
484,274 + -0.7
484,351 + -1.2
484,363 - -0.7
484,378 - +0.1

Or see this region's nucleotide sequence