Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ynfH and dmsD are separated by 42 nucleotides dmsD and clcB are separated by 193 nucleotides clcB and bioD overlap by 48 nucleotides bioD and mlc are separated by 124 nucleotides
MCAODC_17650: ynfH - Anaerobic dimethyl sulfoxide reductase chain YnfH, at 392,680 to 393,534
ynfH
MCAODC_17655: dmsD - Tat proofreading chaperone DmsD, at 393,577 to 394,191
dmsD
MCAODC_17660: clcB - voltage-gated ClC-type chloride channel ClcB, at 394,385 to 395,641
clcB
MCAODC_17665: bioD - dethiobiotin synthase, at 395,594 to 396,289
bioD
MCAODC_17670: mlc - DNA-binding transcriptional repressor Mlc, at 396,414 to 397,634
mlc
Position (kb) 394
395
396 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 393.438 kb on + strand, within ynfH at 393.493 kb on + strand at 393.644 kb on - strand, within dmsD at 393.719 kb on + strand, within dmsD at 393.720 kb on - strand, within dmsD at 394.157 kb on + strand at 394.272 kb on + strand at 394.360 kb on - strand at 394.367 kb on + strand at 394.367 kb on + strand at 394.468 kb on - strand at 394.691 kb on - strand, within clcB at 394.717 kb on - strand, within clcB at 394.916 kb on - strand, within clcB at 395.056 kb on + strand, within clcB at 395.062 kb on + strand, within clcB at 395.106 kb on + strand, within clcB at 395.239 kb on - strand, within clcB at 395.390 kb on + strand, within clcB at 395.390 kb on + strand, within clcB at 395.390 kb on + strand, within clcB at 395.399 kb on - strand, within clcB at 395.409 kb on + strand, within clcB at 395.409 kb on + strand, within clcB at 395.498 kb on - strand, within clcB at 395.514 kb on + strand, within clcB at 395.541 kb on + strand at 395.541 kb on + strand at 395.569 kb on - strand at 395.636 kb on - strand at 395.640 kb on - strand at 395.640 kb on - strand at 395.650 kb on - strand at 395.650 kb on - strand at 396.178 kb on + strand, within bioD at 396.318 kb on + strand at 396.321 kb on - strand at 396.337 kb on + strand at 396.351 kb on + strand at 396.356 kb on + strand at 396.357 kb on - strand at 396.357 kb on - strand at 396.451 kb on + strand at 396.451 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 393,438 + ynfH MCAODC_17650 0.89 +1.5 393,493 + -1.5 393,644 - dmsD MCAODC_17655 0.11 -1.8 393,719 + dmsD MCAODC_17655 0.23 -2.6 393,720 - dmsD MCAODC_17655 0.23 -0.9 394,157 + +2.1 394,272 + -1.0 394,360 - -0.5 394,367 + +0.2 394,367 + -1.1 394,468 - +1.3 394,691 - clcB MCAODC_17660 0.24 +0.4 394,717 - clcB MCAODC_17660 0.26 +0.1 394,916 - clcB MCAODC_17660 0.42 +2.1 395,056 + clcB MCAODC_17660 0.53 -0.1 395,062 + clcB MCAODC_17660 0.54 +3.1 395,106 + clcB MCAODC_17660 0.57 -1.2 395,239 - clcB MCAODC_17660 0.68 -1.2 395,390 + clcB MCAODC_17660 0.80 -0.4 395,390 + clcB MCAODC_17660 0.80 -0.4 395,390 + clcB MCAODC_17660 0.80 +0.4 395,399 - clcB MCAODC_17660 0.81 -0.5 395,409 + clcB MCAODC_17660 0.81 -0.5 395,409 + clcB MCAODC_17660 0.81 +0.3 395,498 - clcB MCAODC_17660 0.89 -0.1 395,514 + clcB MCAODC_17660 0.90 -1.8 395,541 + +0.6 395,541 + +0.0 395,569 - -0.2 395,636 - -3.7 395,640 - +0.1 395,640 - +0.1 395,650 - -0.6 395,650 - -2.0 396,178 + bioD MCAODC_17665 0.84 +1.3 396,318 + +3.4 396,321 - +1.7 396,337 + -0.7 396,351 + +0.2 396,356 + -0.2 396,357 - -1.9 396,357 - -0.7 396,451 + -0.8 396,451 + -0.5
Or see this region's nucleotide sequence