Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_16455 and MCAODC_16460 overlap by 1 nucleotides MCAODC_16460 and ybfL are separated by 507 nucleotides ybfL and tnp-ISAs1 are separated by 99 nucleotides tnp-ISAs1 and pptA are separated by 98 nucleotides pptA and yddH are separated by 3 nucleotides yddH and yddH are separated by 3 nucleotides
MCAODC_16455: MCAODC_16455 - Type IV secretion protein Rhs, at 187,691 to 187,876
_16455
MCAODC_16460: MCAODC_16460 - DsORF-e4, at 187,876 to 188,085
_16460
MCAODC_16465: ybfL - ISAs1 family transposase, at 188,593 to 188,772
ybfL
MCAODC_16470: tnp-ISAs1 - ISAs1 family transposase, at 188,872 to 189,762
tnp-ISAs1
MCAODC_16475: pptA - tautomerase PptA, at 189,861 to 190,088
pptA
MCAODC_16480: yddH - Uncharacterized protein YddH, at 190,092 to 190,535
yddH
MCAODC_16485: yddH - Uncharacterized protein YddH, at 190,539 to 190,661
yddH
Position (kb) 188
189
190 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 189.777 kb on + strand at 189.777 kb on + strand at 189.879 kb on - strand at 190.022 kb on + strand, within pptA at 190.086 kb on + strand at 190.103 kb on + strand at 190.163 kb on + strand, within yddH at 190.247 kb on - strand, within yddH at 190.357 kb on + strand, within yddH at 190.371 kb on - strand, within yddH at 190.415 kb on + strand, within yddH at 190.415 kb on + strand, within yddH at 190.458 kb on - strand, within yddH at 190.491 kb on - strand at 190.554 kb on - strand, within yddH at 190.554 kb on - strand, within yddH at 190.614 kb on + strand, within yddH at 190.636 kb on - strand, within yddH at 190.641 kb on + strand, within yddH at 190.690 kb on - strand at 190.695 kb on + strand at 190.710 kb on + strand at 190.714 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 189,777 + -1.5 189,777 + -0.3 189,879 - -1.7 190,022 + pptA MCAODC_16475 0.71 +0.2 190,086 + +0.5 190,103 + -0.7 190,163 + yddH MCAODC_16480 0.16 +0.5 190,247 - yddH MCAODC_16480 0.35 +2.9 190,357 + yddH MCAODC_16480 0.60 +0.8 190,371 - yddH MCAODC_16480 0.63 +0.2 190,415 + yddH MCAODC_16480 0.73 -0.1 190,415 + yddH MCAODC_16480 0.73 -1.6 190,458 - yddH MCAODC_16480 0.82 -1.5 190,491 - -0.1 190,554 - yddH MCAODC_16485 0.12 -1.8 190,554 - yddH MCAODC_16485 0.12 +1.1 190,614 + yddH MCAODC_16485 0.61 +0.1 190,636 - yddH MCAODC_16485 0.79 -0.5 190,641 + yddH MCAODC_16485 0.83 -1.4 190,690 - +1.1 190,695 + +1.0 190,710 + -0.9 190,714 + -0.2
Or see this region's nucleotide sequence