Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_14960 and MCAODC_14965 are separated by 136 nucleotides MCAODC_14965 and MCAODC_14970 are separated by 40 nucleotides MCAODC_14970 and MCAODC_14975 are separated by 77 nucleotides MCAODC_14975 and MCAODC_14980 are separated by 4 nucleotides MCAODC_14980 and MCAODC_14985 are separated by 270 nucleotides MCAODC_14985 and MCAODC_14990 overlap by 1 nucleotides MCAODC_14990 and MCAODC_14995 are separated by 7 nucleotides
MCAODC_14960: MCAODC_14960 - sialate O-acetylesterase, at 2,925,495 to 2,927,441
_14960
MCAODC_14965: MCAODC_14965 - DUF1378 domain-containing protein, at 2,927,578 to 2,927,757
_14965
MCAODC_14970: MCAODC_14970 - DUF826 domain-containing protein, at 2,927,798 to 2,928,043
_14970
MCAODC_14975: MCAODC_14975 - holin, at 2,928,121 to 2,928,336
_14975
MCAODC_14980: MCAODC_14980 - SAR-endolysin, at 2,928,341 to 2,928,874
_14980
MCAODC_14985: MCAODC_14985 - Phage antirepressor protein, at 2,929,145 to 2,929,714
_14985
MCAODC_14990: MCAODC_14990 - hypothetical protein, at 2,929,714 to 2,929,860
_14990
MCAODC_14995: MCAODC_14995 - lysis protein, at 2,929,868 to 2,930,335
_14995
Position (kb)
2928
2929 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2928.042 kb on - strand at 2928.042 kb on - strand at 2928.042 kb on - strand at 2928.042 kb on - strand at 2928.042 kb on - strand at 2928.042 kb on - strand at 2928.042 kb on - strand at 2928.042 kb on - strand at 2928.042 kb on - strand at 2928.589 kb on + strand, within MCAODC_14980
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 2,928,042 - +2.2 2,928,042 - +1.2 2,928,042 - -0.1 2,928,042 - -0.2 2,928,042 - -0.6 2,928,042 - +0.5 2,928,042 - +2.2 2,928,042 - -2.9 2,928,042 - +0.2 2,928,589 + MCAODC_14980 0.46 -0.7
Or see this region's nucleotide sequence