Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rhmD and MCAODC_14070 are separated by 56 nucleotides MCAODC_14070 and tnp-IS3 are separated by 2 nucleotides tnp-IS3 and tnp-IS3 overlap by 1 nucleotides tnp-IS3 and MCAODC_14085 are separated by 76 nucleotides
MCAODC_14065: rhmD - L-rhamnonate dehydratase, at 2,755,793 to 2,756,998
rhmD
MCAODC_14070: MCAODC_14070 - MFS transporter, at 2,757,055 to 2,757,612
_14070
MCAODC_14075: tnp-IS3 - IS3 family transposase, at 2,757,615 to 2,758,502
tnp-IS3
MCAODC_14080: tnp-IS3 - IS3 family transposase, at 2,758,502 to 2,758,828
tnp-IS3
MCAODC_14085: MCAODC_14085 - MFS transporter, at 2,758,905 to 2,759,657
_14085
Position (kb)
2757
2758
2759 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2756.674 kb on + strand, within rhmD at 2756.674 kb on + strand, within rhmD at 2756.809 kb on + strand, within rhmD at 2756.810 kb on - strand, within rhmD at 2756.810 kb on - strand, within rhmD at 2756.810 kb on - strand, within rhmD at 2756.839 kb on + strand, within rhmD at 2756.840 kb on - strand, within rhmD at 2756.914 kb on + strand at 2756.920 kb on + strand at 2756.921 kb on - strand at 2756.935 kb on + strand at 2756.935 kb on + strand at 2757.037 kb on + strand at 2757.038 kb on - strand at 2757.042 kb on + strand at 2757.043 kb on - strand at 2757.043 kb on - strand at 2757.054 kb on + strand at 2757.055 kb on - strand at 2757.127 kb on + strand, within MCAODC_14070 at 2757.127 kb on + strand, within MCAODC_14070 at 2757.128 kb on - strand, within MCAODC_14070 at 2757.128 kb on - strand, within MCAODC_14070 at 2757.128 kb on - strand, within MCAODC_14070 at 2757.211 kb on + strand, within MCAODC_14070 at 2757.242 kb on + strand, within MCAODC_14070 at 2757.242 kb on + strand, within MCAODC_14070 at 2757.242 kb on + strand, within MCAODC_14070 at 2757.243 kb on - strand, within MCAODC_14070 at 2757.243 kb on - strand, within MCAODC_14070 at 2757.243 kb on - strand, within MCAODC_14070 at 2757.243 kb on - strand, within MCAODC_14070 at 2757.243 kb on - strand, within MCAODC_14070 at 2757.244 kb on + strand, within MCAODC_14070 at 2757.244 kb on + strand, within MCAODC_14070 at 2757.244 kb on + strand, within MCAODC_14070 at 2757.380 kb on + strand, within MCAODC_14070 at 2757.380 kb on + strand, within MCAODC_14070 at 2757.427 kb on - strand, within MCAODC_14070 at 2757.490 kb on + strand, within MCAODC_14070 at 2757.490 kb on + strand, within MCAODC_14070 at 2757.490 kb on + strand, within MCAODC_14070 at 2757.490 kb on + strand, within MCAODC_14070 at 2757.491 kb on - strand, within MCAODC_14070 at 2757.491 kb on - strand, within MCAODC_14070 at 2757.491 kb on - strand, within MCAODC_14070 at 2757.513 kb on - strand, within MCAODC_14070 at 2758.855 kb on + strand at 2759.076 kb on - strand, within MCAODC_14085 at 2759.249 kb on + strand, within MCAODC_14085 at 2759.324 kb on + strand, within MCAODC_14085 at 2759.324 kb on + strand, within MCAODC_14085 at 2759.325 kb on - strand, within MCAODC_14085 at 2759.325 kb on - strand, within MCAODC_14085 at 2759.394 kb on - strand, within MCAODC_14085 at 2759.473 kb on + strand, within MCAODC_14085 at 2759.501 kb on + strand, within MCAODC_14085 at 2759.502 kb on - strand, within MCAODC_14085
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 2,756,674 + rhmD MCAODC_14065 0.73 +0.6 2,756,674 + rhmD MCAODC_14065 0.73 +0.8 2,756,809 + rhmD MCAODC_14065 0.84 -2.0 2,756,810 - rhmD MCAODC_14065 0.84 -1.3 2,756,810 - rhmD MCAODC_14065 0.84 +0.2 2,756,810 - rhmD MCAODC_14065 0.84 -0.8 2,756,839 + rhmD MCAODC_14065 0.87 +0.7 2,756,840 - rhmD MCAODC_14065 0.87 -0.5 2,756,914 + +0.5 2,756,920 + +0.6 2,756,921 - -3.2 2,756,935 + -0.2 2,756,935 + -1.7 2,757,037 + -1.5 2,757,038 - +2.3 2,757,042 + +1.8 2,757,043 - -1.1 2,757,043 - -0.5 2,757,054 + +1.7 2,757,055 - -0.7 2,757,127 + MCAODC_14070 0.13 +1.6 2,757,127 + MCAODC_14070 0.13 -0.3 2,757,128 - MCAODC_14070 0.13 -0.4 2,757,128 - MCAODC_14070 0.13 +1.2 2,757,128 - MCAODC_14070 0.13 +1.0 2,757,211 + MCAODC_14070 0.28 -2.8 2,757,242 + MCAODC_14070 0.34 -0.9 2,757,242 + MCAODC_14070 0.34 +0.5 2,757,242 + MCAODC_14070 0.34 +1.1 2,757,243 - MCAODC_14070 0.34 -0.6 2,757,243 - MCAODC_14070 0.34 +0.4 2,757,243 - MCAODC_14070 0.34 -3.0 2,757,243 - MCAODC_14070 0.34 +0.7 2,757,243 - MCAODC_14070 0.34 +2.8 2,757,244 + MCAODC_14070 0.34 -0.3 2,757,244 + MCAODC_14070 0.34 +0.9 2,757,244 + MCAODC_14070 0.34 +0.2 2,757,380 + MCAODC_14070 0.58 -1.0 2,757,380 + MCAODC_14070 0.58 +0.8 2,757,427 - MCAODC_14070 0.67 +0.2 2,757,490 + MCAODC_14070 0.78 -1.4 2,757,490 + MCAODC_14070 0.78 -0.2 2,757,490 + MCAODC_14070 0.78 +0.2 2,757,490 + MCAODC_14070 0.78 +1.8 2,757,491 - MCAODC_14070 0.78 +0.9 2,757,491 - MCAODC_14070 0.78 +0.3 2,757,491 - MCAODC_14070 0.78 -0.3 2,757,513 - MCAODC_14070 0.82 +3.8 2,758,855 + -1.1 2,759,076 - MCAODC_14085 0.23 +1.8 2,759,249 + MCAODC_14085 0.46 -0.2 2,759,324 + MCAODC_14085 0.56 -2.1 2,759,324 + MCAODC_14085 0.56 -1.0 2,759,325 - MCAODC_14085 0.56 +1.7 2,759,325 - MCAODC_14085 0.56 +0.3 2,759,394 - MCAODC_14085 0.65 -1.3 2,759,473 + MCAODC_14085 0.75 -2.1 2,759,501 + MCAODC_14085 0.79 -1.8 2,759,502 - MCAODC_14085 0.79 -1.4
Or see this region's nucleotide sequence