Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt actS and Gly_trna are separated by 78 nucleotides Gly_trna and MCAODC_10250 are separated by 233 nucleotides MCAODC_10250 and MCAODC_10255 overlap by 17 nucleotides MCAODC_10255 and MCAODC_10260 are separated by 66 nucleotides MCAODC_10260 and araC are separated by 345 nucleotides
MCAODC_10240: actS - amidase activator ActS, at 2,046,576 to 2,047,331
actS
MCAODC_10245: Gly_trna - tRNA-Gly(ccc), at 2,047,410 to 2,047,483
_trna
MCAODC_10250: MCAODC_10250 - Glyco-hydro-15 domain-containing protein, at 2,047,717 to 2,048,280
_10250
MCAODC_10255: MCAODC_10255 - Glyco-hydro-15 domain-containing protein, at 2,048,264 to 2,049,322
_10255
MCAODC_10260: MCAODC_10260 - GhoT/OrtT family toxin, at 2,049,389 to 2,049,568
_10260
MCAODC_10265: araC - AraC family transcriptional regulator, at 2,049,914 to 2,050,663
araC
Position (kb)
2048
2049
2050 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2047.296 kb on + strand at 2047.297 kb on - strand at 2047.297 kb on - strand at 2047.297 kb on - strand at 2047.380 kb on + strand at 2047.388 kb on - strand at 2047.389 kb on + strand at 2047.389 kb on + strand at 2047.390 kb on - strand at 2047.417 kb on + strand at 2047.451 kb on + strand, within Gly_trna at 2047.453 kb on + strand, within Gly_trna at 2047.453 kb on + strand, within Gly_trna at 2047.520 kb on + strand at 2047.535 kb on - strand at 2047.602 kb on - strand at 2047.681 kb on - strand at 2047.816 kb on - strand, within MCAODC_10250 at 2047.816 kb on - strand, within MCAODC_10250 at 2047.875 kb on + strand, within MCAODC_10250 at 2048.037 kb on + strand, within MCAODC_10250 at 2048.038 kb on - strand, within MCAODC_10250 at 2048.074 kb on + strand, within MCAODC_10250 at 2048.074 kb on + strand, within MCAODC_10250 at 2048.098 kb on - strand, within MCAODC_10250 at 2048.111 kb on - strand, within MCAODC_10250 at 2048.111 kb on - strand, within MCAODC_10250 at 2048.112 kb on + strand, within MCAODC_10250 at 2048.120 kb on + strand, within MCAODC_10250 at 2048.140 kb on - strand, within MCAODC_10250 at 2048.179 kb on + strand, within MCAODC_10250 at 2048.180 kb on - strand, within MCAODC_10250 at 2048.180 kb on - strand, within MCAODC_10250 at 2048.181 kb on + strand, within MCAODC_10250 at 2048.181 kb on + strand, within MCAODC_10250 at 2048.182 kb on - strand, within MCAODC_10250 at 2048.223 kb on + strand, within MCAODC_10250 at 2048.275 kb on - strand at 2048.358 kb on + strand at 2048.358 kb on + strand at 2048.366 kb on + strand at 2048.383 kb on - strand, within MCAODC_10255 at 2048.383 kb on - strand, within MCAODC_10255 at 2048.401 kb on + strand, within MCAODC_10255 at 2048.404 kb on + strand, within MCAODC_10255 at 2048.404 kb on + strand, within MCAODC_10255 at 2048.405 kb on - strand, within MCAODC_10255 at 2048.407 kb on + strand, within MCAODC_10255 at 2048.408 kb on - strand, within MCAODC_10255 at 2048.408 kb on - strand, within MCAODC_10255 at 2048.465 kb on + strand, within MCAODC_10255 at 2048.466 kb on - strand, within MCAODC_10255 at 2048.622 kb on + strand, within MCAODC_10255 at 2048.632 kb on + strand, within MCAODC_10255 at 2048.632 kb on + strand, within MCAODC_10255 at 2048.690 kb on - strand, within MCAODC_10255 at 2049.101 kb on - strand, within MCAODC_10255 at 2049.194 kb on + strand, within MCAODC_10255 at 2049.195 kb on - strand, within MCAODC_10255 at 2049.471 kb on + strand, within MCAODC_10260 at 2049.713 kb on + strand at 2049.790 kb on + strand at 2049.933 kb on + strand at 2050.177 kb on + strand, within araC
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 2,047,296 + -0.9 2,047,297 - +0.3 2,047,297 - -0.4 2,047,297 - -0.1 2,047,380 + -2.6 2,047,388 - +0.5 2,047,389 + -1.5 2,047,389 + +0.1 2,047,390 - -1.3 2,047,417 + -0.6 2,047,451 + Gly_trna MCAODC_10245 0.55 -2.1 2,047,453 + Gly_trna MCAODC_10245 0.58 +0.4 2,047,453 + Gly_trna MCAODC_10245 0.58 +0.8 2,047,520 + -1.2 2,047,535 - +0.4 2,047,602 - -0.4 2,047,681 - +0.4 2,047,816 - MCAODC_10250 0.18 +0.0 2,047,816 - MCAODC_10250 0.18 +0.6 2,047,875 + MCAODC_10250 0.28 -2.3 2,048,037 + MCAODC_10250 0.57 -0.7 2,048,038 - MCAODC_10250 0.57 -1.5 2,048,074 + MCAODC_10250 0.63 +0.5 2,048,074 + MCAODC_10250 0.63 +0.9 2,048,098 - MCAODC_10250 0.68 -0.3 2,048,111 - MCAODC_10250 0.70 -1.8 2,048,111 - MCAODC_10250 0.70 +1.9 2,048,112 + MCAODC_10250 0.70 -0.9 2,048,120 + MCAODC_10250 0.71 -1.9 2,048,140 - MCAODC_10250 0.75 -0.4 2,048,179 + MCAODC_10250 0.82 +0.6 2,048,180 - MCAODC_10250 0.82 +0.3 2,048,180 - MCAODC_10250 0.82 +0.5 2,048,181 + MCAODC_10250 0.82 +0.9 2,048,181 + MCAODC_10250 0.82 +0.0 2,048,182 - MCAODC_10250 0.82 -0.1 2,048,223 + MCAODC_10250 0.90 -0.7 2,048,275 - -1.4 2,048,358 + +0.7 2,048,358 + +0.8 2,048,366 + -0.0 2,048,383 - MCAODC_10255 0.11 +1.0 2,048,383 - MCAODC_10255 0.11 -3.3 2,048,401 + MCAODC_10255 0.13 -0.1 2,048,404 + MCAODC_10255 0.13 -2.6 2,048,404 + MCAODC_10255 0.13 +0.1 2,048,405 - MCAODC_10255 0.13 +0.2 2,048,407 + MCAODC_10255 0.14 +0.0 2,048,408 - MCAODC_10255 0.14 +1.0 2,048,408 - MCAODC_10255 0.14 -1.6 2,048,465 + MCAODC_10255 0.19 -0.5 2,048,466 - MCAODC_10255 0.19 +1.0 2,048,622 + MCAODC_10255 0.34 +1.3 2,048,632 + MCAODC_10255 0.35 -1.2 2,048,632 + MCAODC_10255 0.35 +0.6 2,048,690 - MCAODC_10255 0.40 +0.8 2,049,101 - MCAODC_10255 0.79 -3.1 2,049,194 + MCAODC_10255 0.88 -0.6 2,049,195 - MCAODC_10255 0.88 +0.3 2,049,471 + MCAODC_10260 0.46 +3.6 2,049,713 + +0.8 2,049,790 + -2.8 2,049,933 + -0.2 2,050,177 + araC MCAODC_10265 0.35 +0.2
Or see this region's nucleotide sequence