Strain Fitness in Escherichia coli ECRC101 around MCAODC_08185

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntacrE and acrS are separated by 398 nucleotidesacrS and yhdU are separated by 2 nucleotidesyhdU and yhdJ are separated by 83 nucleotides MCAODC_08180: acrE - multidrug efflux RND transporter periplasmic adaptor subunit AcrE, at 1,636,215 to 1,637,372 acrE MCAODC_08185: acrS - multidrug efflux transporter transcriptional repressor AcrS, at 1,637,771 to 1,638,433 acrS MCAODC_08190: yhdU - Inner membrane protein YhdU, at 1,638,436 to 1,638,615 yhdU MCAODC_08195: yhdJ - adenine-specific DNA-methyltransferase, at 1,638,699 to 1,639,583 yhdJ Position (kb) 1637 1638 1639Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1636.827 kb on - strand, within acrEat 1636.836 kb on - strand, within acrEat 1636.857 kb on - strand, within acrEat 1636.857 kb on - strand, within acrEat 1636.857 kb on - strand, within acrEat 1636.858 kb on + strand, within acrEat 1636.858 kb on + strand, within acrEat 1636.858 kb on + strand, within acrEat 1636.858 kb on + strand, within acrEat 1636.858 kb on + strand, within acrEat 1636.859 kb on - strand, within acrEat 1636.859 kb on - strand, within acrEat 1636.859 kb on - strand, within acrEat 1636.967 kb on - strand, within acrEat 1636.967 kb on - strand, within acrEat 1636.967 kb on - strand, within acrEat 1636.967 kb on - strand, within acrEat 1636.967 kb on - strand, within acrEat 1637.063 kb on - strand, within acrEat 1637.149 kb on - strand, within acrEat 1637.183 kb on + strand, within acrEat 1637.183 kb on + strand, within acrEat 1637.184 kb on - strand, within acrEat 1637.184 kb on - strand, within acrEat 1637.184 kb on - strand, within acrEat 1637.208 kb on + strand, within acrEat 1637.376 kb on + strandat 1637.377 kb on - strandat 1637.606 kb on + strandat 1637.642 kb on - strandat 1637.899 kb on + strand, within acrSat 1637.912 kb on - strand, within acrSat 1637.912 kb on - strand, within acrSat 1637.915 kb on + strand, within acrSat 1637.916 kb on - strand, within acrSat 1637.916 kb on - strand, within acrSat 1637.916 kb on - strand, within acrSat 1637.947 kb on + strand, within acrSat 1638.065 kb on + strand, within acrSat 1638.066 kb on - strand, within acrSat 1638.108 kb on + strand, within acrSat 1638.246 kb on + strand, within acrSat 1638.246 kb on + strand, within acrSat 1638.266 kb on - strand, within acrSat 1638.360 kb on + strand, within acrSat 1638.361 kb on - strand, within acrSat 1638.558 kb on + strand, within yhdUat 1638.602 kb on + strandat 1638.602 kb on + strandat 1638.602 kb on + strandat 1638.602 kb on + strandat 1638.603 kb on - strandat 1638.603 kb on - strandat 1638.616 kb on + strandat 1638.658 kb on + strandat 1638.689 kb on + strandat 1638.690 kb on - strandat 1638.690 kb on - strandat 1638.700 kb on + strandat 1638.707 kb on + strandat 1638.799 kb on + strand, within yhdJat 1638.799 kb on + strand, within yhdJat 1638.799 kb on + strand, within yhdJat 1638.842 kb on - strand, within yhdJat 1638.861 kb on - strand, within yhdJat 1638.891 kb on + strand, within yhdJat 1638.906 kb on - strand, within yhdJat 1638.906 kb on - strand, within yhdJat 1638.906 kb on - strand, within yhdJat 1638.906 kb on - strand, within yhdJat 1638.912 kb on - strand, within yhdJat 1639.025 kb on - strand, within yhdJat 1639.075 kb on + strand, within yhdJat 1639.075 kb on + strand, within yhdJat 1639.075 kb on + strand, within yhdJat 1639.075 kb on + strand, within yhdJat 1639.076 kb on - strand, within yhdJat 1639.076 kb on - strand, within yhdJat 1639.138 kb on - strand, within yhdJat 1639.145 kb on + strand, within yhdJat 1639.146 kb on - strand, within yhdJat 1639.165 kb on + strand, within yhdJat 1639.204 kb on + strand, within yhdJat 1639.204 kb on + strand, within yhdJat 1639.205 kb on - strand, within yhdJat 1639.205 kb on - strand, within yhdJat 1639.205 kb on - strand, within yhdJat 1639.205 kb on - strand, within yhdJat 1639.205 kb on - strand, within yhdJat 1639.205 kb on - strand, within yhdJat 1639.249 kb on + strand, within yhdJat 1639.277 kb on + strand, within yhdJat 1639.336 kb on + strand, within yhdJat 1639.336 kb on + strand, within yhdJat 1639.336 kb on + strand, within yhdJat 1639.336 kb on + strand, within yhdJat 1639.336 kb on + strand, within yhdJat 1639.336 kb on + strand, within yhdJat 1639.337 kb on - strand, within yhdJat 1639.337 kb on - strand, within yhdJat 1639.387 kb on - strand, within yhdJ

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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1,636,827 - acrE MCAODC_08180 0.53 +0.4
1,636,836 - acrE MCAODC_08180 0.54 -0.4
1,636,857 - acrE MCAODC_08180 0.55 -0.6
1,636,857 - acrE MCAODC_08180 0.55 -0.1
1,636,857 - acrE MCAODC_08180 0.55 -1.5
1,636,858 + acrE MCAODC_08180 0.56 +0.2
1,636,858 + acrE MCAODC_08180 0.56 -2.9
1,636,858 + acrE MCAODC_08180 0.56 +0.8
1,636,858 + acrE MCAODC_08180 0.56 +1.4
1,636,858 + acrE MCAODC_08180 0.56 -1.3
1,636,859 - acrE MCAODC_08180 0.56 -2.5
1,636,859 - acrE MCAODC_08180 0.56 +0.1
1,636,859 - acrE MCAODC_08180 0.56 +0.3
1,636,967 - acrE MCAODC_08180 0.65 -0.2
1,636,967 - acrE MCAODC_08180 0.65 +1.1
1,636,967 - acrE MCAODC_08180 0.65 +0.0
1,636,967 - acrE MCAODC_08180 0.65 +0.6
1,636,967 - acrE MCAODC_08180 0.65 -0.7
1,637,063 - acrE MCAODC_08180 0.73 -2.9
1,637,149 - acrE MCAODC_08180 0.81 +1.1
1,637,183 + acrE MCAODC_08180 0.84 +0.8
1,637,183 + acrE MCAODC_08180 0.84 -2.0
1,637,184 - acrE MCAODC_08180 0.84 +0.1
1,637,184 - acrE MCAODC_08180 0.84 +0.2
1,637,184 - acrE MCAODC_08180 0.84 +0.1
1,637,208 + acrE MCAODC_08180 0.86 +0.5
1,637,376 + -0.4
1,637,377 - +0.9
1,637,606 + +1.4
1,637,642 - -0.8
1,637,899 + acrS MCAODC_08185 0.19 +0.3
1,637,912 - acrS MCAODC_08185 0.21 -0.1
1,637,912 - acrS MCAODC_08185 0.21 -1.5
1,637,915 + acrS MCAODC_08185 0.22 +1.8
1,637,916 - acrS MCAODC_08185 0.22 +1.7
1,637,916 - acrS MCAODC_08185 0.22 -2.0
1,637,916 - acrS MCAODC_08185 0.22 -0.4
1,637,947 + acrS MCAODC_08185 0.27 -0.6
1,638,065 + acrS MCAODC_08185 0.44 +0.3
1,638,066 - acrS MCAODC_08185 0.44 +0.1
1,638,108 + acrS MCAODC_08185 0.51 +0.3
1,638,246 + acrS MCAODC_08185 0.72 +0.4
1,638,246 + acrS MCAODC_08185 0.72 -1.0
1,638,266 - acrS MCAODC_08185 0.75 -0.5
1,638,360 + acrS MCAODC_08185 0.89 -0.0
1,638,361 - acrS MCAODC_08185 0.89 +2.1
1,638,558 + yhdU MCAODC_08190 0.68 -0.2
1,638,602 + +0.3
1,638,602 + -1.4
1,638,602 + -0.4
1,638,602 + -1.2
1,638,603 - -0.1
1,638,603 - -0.2
1,638,616 + -0.0
1,638,658 + +0.1
1,638,689 + +1.3
1,638,690 - +1.1
1,638,690 - +1.5
1,638,700 + -0.2
1,638,707 + +0.1
1,638,799 + yhdJ MCAODC_08195 0.11 -1.3
1,638,799 + yhdJ MCAODC_08195 0.11 +0.2
1,638,799 + yhdJ MCAODC_08195 0.11 -1.8
1,638,842 - yhdJ MCAODC_08195 0.16 -3.3
1,638,861 - yhdJ MCAODC_08195 0.18 +1.0
1,638,891 + yhdJ MCAODC_08195 0.22 +0.6
1,638,906 - yhdJ MCAODC_08195 0.23 -1.2
1,638,906 - yhdJ MCAODC_08195 0.23 +0.4
1,638,906 - yhdJ MCAODC_08195 0.23 -0.8
1,638,906 - yhdJ MCAODC_08195 0.23 -0.6
1,638,912 - yhdJ MCAODC_08195 0.24 -3.0
1,639,025 - yhdJ MCAODC_08195 0.37 -1.2
1,639,075 + yhdJ MCAODC_08195 0.42 -2.0
1,639,075 + yhdJ MCAODC_08195 0.42 +1.4
1,639,075 + yhdJ MCAODC_08195 0.42 +0.3
1,639,075 + yhdJ MCAODC_08195 0.42 -0.3
1,639,076 - yhdJ MCAODC_08195 0.43 +0.6
1,639,076 - yhdJ MCAODC_08195 0.43 +0.8
1,639,138 - yhdJ MCAODC_08195 0.50 +0.7
1,639,145 + yhdJ MCAODC_08195 0.50 -1.2
1,639,146 - yhdJ MCAODC_08195 0.51 +0.2
1,639,165 + yhdJ MCAODC_08195 0.53 +0.0
1,639,204 + yhdJ MCAODC_08195 0.57 +0.0
1,639,204 + yhdJ MCAODC_08195 0.57 -1.2
1,639,205 - yhdJ MCAODC_08195 0.57 +0.2
1,639,205 - yhdJ MCAODC_08195 0.57 +1.9
1,639,205 - yhdJ MCAODC_08195 0.57 -1.1
1,639,205 - yhdJ MCAODC_08195 0.57 +0.5
1,639,205 - yhdJ MCAODC_08195 0.57 -1.2
1,639,205 - yhdJ MCAODC_08195 0.57 -0.5
1,639,249 + yhdJ MCAODC_08195 0.62 +0.1
1,639,277 + yhdJ MCAODC_08195 0.65 -0.5
1,639,336 + yhdJ MCAODC_08195 0.72 -0.8
1,639,336 + yhdJ MCAODC_08195 0.72 +1.6
1,639,336 + yhdJ MCAODC_08195 0.72 +0.5
1,639,336 + yhdJ MCAODC_08195 0.72 -0.1
1,639,336 + yhdJ MCAODC_08195 0.72 +0.6
1,639,336 + yhdJ MCAODC_08195 0.72 +0.5
1,639,337 - yhdJ MCAODC_08195 0.72 -0.2
1,639,337 - yhdJ MCAODC_08195 0.72 +0.3
1,639,387 - yhdJ MCAODC_08195 0.78 +0.6

Or see this region's nucleotide sequence