Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_04255 and MCAODC_04260 are separated by 122 nucleotides MCAODC_04260 and zapB are separated by 38 nucleotides zapB and glpF are separated by 431 nucleotides
MCAODC_04255: MCAODC_04255 - hypothetical protein, at 865,875 to 866,549
_04255
MCAODC_04260: MCAODC_04260 - hypothetical protein, at 866,672 to 867,298
_04260
MCAODC_04265: zapB - septal ring assembly protein ZapB, at 867,337 to 867,576
zapB
MCAODC_04270: glpF - glycerol uptake facilitator protein GlpF, at 868,008 to 868,853
glpF
Position (kb) 867
868 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 866.420 kb on - strand, within MCAODC_04255 at 866.429 kb on + strand, within MCAODC_04255 at 866.518 kb on + strand at 866.518 kb on + strand at 866.567 kb on + strand at 866.571 kb on - strand at 866.589 kb on - strand at 866.593 kb on + strand at 866.594 kb on - strand at 866.605 kb on - strand at 866.605 kb on - strand at 866.608 kb on - strand at 866.629 kb on + strand at 866.648 kb on - strand at 866.657 kb on - strand at 866.697 kb on + strand at 866.698 kb on - strand at 866.737 kb on + strand, within MCAODC_04260 at 866.738 kb on - strand, within MCAODC_04260 at 866.782 kb on + strand, within MCAODC_04260 at 866.782 kb on + strand, within MCAODC_04260 at 866.821 kb on - strand, within MCAODC_04260 at 866.865 kb on - strand, within MCAODC_04260 at 866.867 kb on - strand, within MCAODC_04260 at 866.867 kb on - strand, within MCAODC_04260 at 866.885 kb on - strand, within MCAODC_04260 at 866.885 kb on - strand, within MCAODC_04260 at 866.885 kb on - strand, within MCAODC_04260 at 866.932 kb on + strand, within MCAODC_04260 at 866.932 kb on + strand, within MCAODC_04260 at 866.932 kb on + strand, within MCAODC_04260 at 866.950 kb on + strand, within MCAODC_04260 at 866.950 kb on + strand, within MCAODC_04260 at 866.951 kb on - strand, within MCAODC_04260 at 866.952 kb on + strand, within MCAODC_04260 at 866.952 kb on + strand, within MCAODC_04260 at 866.953 kb on - strand, within MCAODC_04260 at 866.953 kb on - strand, within MCAODC_04260 at 866.966 kb on - strand, within MCAODC_04260 at 866.966 kb on - strand, within MCAODC_04260 at 866.966 kb on - strand, within MCAODC_04260 at 866.966 kb on - strand, within MCAODC_04260 at 866.968 kb on + strand, within MCAODC_04260 at 866.977 kb on + strand, within MCAODC_04260 at 867.008 kb on - strand, within MCAODC_04260 at 867.008 kb on - strand, within MCAODC_04260 at 867.008 kb on - strand, within MCAODC_04260 at 867.033 kb on + strand, within MCAODC_04260 at 867.068 kb on + strand, within MCAODC_04260 at 867.068 kb on + strand, within MCAODC_04260 at 867.100 kb on + strand, within MCAODC_04260 at 867.101 kb on - strand, within MCAODC_04260 at 867.101 kb on - strand, within MCAODC_04260 at 867.101 kb on - strand, within MCAODC_04260 at 867.121 kb on + strand, within MCAODC_04260 at 867.121 kb on + strand, within MCAODC_04260 at 867.121 kb on + strand, within MCAODC_04260 at 867.122 kb on - strand, within MCAODC_04260 at 867.122 kb on - strand, within MCAODC_04260 at 867.122 kb on - strand, within MCAODC_04260 at 867.125 kb on + strand, within MCAODC_04260 at 867.142 kb on - strand, within MCAODC_04260 at 867.180 kb on - strand, within MCAODC_04260 at 867.220 kb on - strand, within MCAODC_04260 at 867.232 kb on + strand, within MCAODC_04260 at 867.233 kb on - strand, within MCAODC_04260 at 867.241 kb on + strand at 867.241 kb on + strand at 867.242 kb on - strand at 867.242 kb on - strand at 867.242 kb on - strand at 867.268 kb on + strand at 867.268 kb on + strand at 867.268 kb on + strand at 867.268 kb on + strand at 867.268 kb on + strand at 867.268 kb on + strand at 867.268 kb on + strand at 867.268 kb on + strand at 867.268 kb on + strand at 867.268 kb on + strand at 867.269 kb on - strand at 867.269 kb on - strand at 867.269 kb on - strand at 867.269 kb on - strand at 867.269 kb on - strand at 867.269 kb on - strand at 867.287 kb on - strand at 867.287 kb on - strand at 867.338 kb on + strand at 867.338 kb on + strand at 867.339 kb on - strand at 867.539 kb on - strand, within zapB at 867.568 kb on + strand at 867.569 kb on - strand at 867.569 kb on - strand at 867.569 kb on - strand at 867.569 kb on - strand at 867.582 kb on + strand at 867.582 kb on + strand at 867.582 kb on + strand at 867.582 kb on + strand at 867.583 kb on - strand at 867.583 kb on - strand at 867.583 kb on - strand at 867.647 kb on + strand at 867.650 kb on + strand at 867.650 kb on + strand at 867.650 kb on + strand at 867.651 kb on - strand at 867.651 kb on - strand at 867.651 kb on - strand at 867.654 kb on + strand at 867.654 kb on + strand at 867.668 kb on - strand at 867.672 kb on - strand at 867.683 kb on + strand at 867.684 kb on - strand at 867.684 kb on - strand at 867.764 kb on + strand at 867.765 kb on - strand at 867.787 kb on + strand at 867.891 kb on - strand at 867.942 kb on + strand at 867.943 kb on - strand at 867.976 kb on + strand at 867.976 kb on + strand at 867.976 kb on + strand at 867.977 kb on - strand at 867.977 kb on - strand at 867.977 kb on - strand at 867.977 kb on - strand at 867.977 kb on - strand at 867.988 kb on + strand at 867.989 kb on - strand at 868.008 kb on - strand at 868.102 kb on + strand, within glpF at 868.103 kb on - strand, within glpF at 868.103 kb on - strand, within glpF at 868.176 kb on + strand, within glpF at 868.176 kb on + strand, within glpF at 868.176 kb on + strand, within glpF at 868.209 kb on - strand, within glpF at 868.220 kb on + strand, within glpF at 868.221 kb on - strand, within glpF at 868.274 kb on + strand, within glpF at 868.316 kb on - strand, within glpF at 868.329 kb on + strand, within glpF at 868.332 kb on + strand, within glpF at 868.332 kb on + strand, within glpF at 868.415 kb on + strand, within glpF at 868.416 kb on - strand, within glpF at 868.419 kb on + strand, within glpF at 868.419 kb on + strand, within glpF at 868.419 kb on + strand, within glpF at 868.420 kb on - strand, within glpF at 868.420 kb on - strand, within glpF
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 866,420 - MCAODC_04255 0.81 +0.2 866,429 + MCAODC_04255 0.82 +1.2 866,518 + +0.6 866,518 + +0.3 866,567 + -1.0 866,571 - -1.6 866,589 - -1.4 866,593 + -0.2 866,594 - -3.6 866,605 - +0.0 866,605 - +0.4 866,608 - +0.4 866,629 + -1.6 866,648 - -0.9 866,657 - +2.1 866,697 + +1.1 866,698 - +0.6 866,737 + MCAODC_04260 0.10 -0.9 866,738 - MCAODC_04260 0.11 +1.6 866,782 + MCAODC_04260 0.18 -0.3 866,782 + MCAODC_04260 0.18 +2.0 866,821 - MCAODC_04260 0.24 +1.5 866,865 - MCAODC_04260 0.31 -2.6 866,867 - MCAODC_04260 0.31 +0.0 866,867 - MCAODC_04260 0.31 +0.3 866,885 - MCAODC_04260 0.34 -3.0 866,885 - MCAODC_04260 0.34 +0.5 866,885 - MCAODC_04260 0.34 +1.7 866,932 + MCAODC_04260 0.41 +1.0 866,932 + MCAODC_04260 0.41 -0.5 866,932 + MCAODC_04260 0.41 +1.6 866,950 + MCAODC_04260 0.44 -0.1 866,950 + MCAODC_04260 0.44 +0.3 866,951 - MCAODC_04260 0.44 -0.4 866,952 + MCAODC_04260 0.45 +0.9 866,952 + MCAODC_04260 0.45 +0.6 866,953 - MCAODC_04260 0.45 +0.1 866,953 - MCAODC_04260 0.45 -3.1 866,966 - MCAODC_04260 0.47 -1.0 866,966 - MCAODC_04260 0.47 -2.7 866,966 - MCAODC_04260 0.47 -1.3 866,966 - MCAODC_04260 0.47 +0.8 866,968 + MCAODC_04260 0.47 -1.3 866,977 + MCAODC_04260 0.49 +0.4 867,008 - MCAODC_04260 0.54 +0.4 867,008 - MCAODC_04260 0.54 +0.2 867,008 - MCAODC_04260 0.54 -0.4 867,033 + MCAODC_04260 0.58 -0.6 867,068 + MCAODC_04260 0.63 +1.2 867,068 + MCAODC_04260 0.63 +0.4 867,100 + MCAODC_04260 0.68 -0.1 867,101 - MCAODC_04260 0.68 +0.8 867,101 - MCAODC_04260 0.68 -0.8 867,101 - MCAODC_04260 0.68 +0.6 867,121 + MCAODC_04260 0.72 +0.8 867,121 + MCAODC_04260 0.72 -0.3 867,121 + MCAODC_04260 0.72 -0.2 867,122 - MCAODC_04260 0.72 -1.3 867,122 - MCAODC_04260 0.72 +0.0 867,122 - MCAODC_04260 0.72 +0.6 867,125 + MCAODC_04260 0.72 -0.7 867,142 - MCAODC_04260 0.75 +0.7 867,180 - MCAODC_04260 0.81 +1.0 867,220 - MCAODC_04260 0.87 +1.3 867,232 + MCAODC_04260 0.89 +0.5 867,233 - MCAODC_04260 0.89 -0.7 867,241 + +0.9 867,241 + +1.3 867,242 - +0.1 867,242 - +1.0 867,242 - +1.9 867,268 + +2.6 867,268 + -0.4 867,268 + +0.8 867,268 + +0.8 867,268 + -1.1 867,268 + -0.4 867,268 + +0.4 867,268 + -0.3 867,268 + -0.7 867,268 + +0.2 867,269 - +0.5 867,269 - -0.0 867,269 - -1.4 867,269 - +0.7 867,269 - -0.2 867,269 - -1.7 867,287 - +0.5 867,287 - +0.4 867,338 + -0.8 867,338 + +0.4 867,339 - +2.4 867,539 - zapB MCAODC_04265 0.84 -0.6 867,568 + +0.8 867,569 - -0.6 867,569 - +0.2 867,569 - +0.4 867,569 - -4.3 867,582 + +0.4 867,582 + +0.4 867,582 + +0.9 867,582 + +2.2 867,583 - -0.1 867,583 - +0.4 867,583 - +0.9 867,647 + -3.3 867,650 + -0.1 867,650 + -0.1 867,650 + -0.1 867,651 - +0.8 867,651 - -1.6 867,651 - +1.4 867,654 + +3.1 867,654 + +0.4 867,668 - -0.5 867,672 - -3.0 867,683 + +0.5 867,684 - +1.5 867,684 - +0.1 867,764 + +0.2 867,765 - +0.9 867,787 + -0.4 867,891 - +1.3 867,942 + +1.2 867,943 - -0.4 867,976 + +2.3 867,976 + +0.9 867,976 + +0.1 867,977 - +0.3 867,977 - +0.1 867,977 - +0.4 867,977 - +0.3 867,977 - -1.7 867,988 + +0.2 867,989 - +1.4 868,008 - +0.7 868,102 + glpF MCAODC_04270 0.11 -3.5 868,103 - glpF MCAODC_04270 0.11 +1.3 868,103 - glpF MCAODC_04270 0.11 +0.4 868,176 + glpF MCAODC_04270 0.20 +1.7 868,176 + glpF MCAODC_04270 0.20 +1.4 868,176 + glpF MCAODC_04270 0.20 -2.8 868,209 - glpF MCAODC_04270 0.24 -0.6 868,220 + glpF MCAODC_04270 0.25 -2.6 868,221 - glpF MCAODC_04270 0.25 +1.9 868,274 + glpF MCAODC_04270 0.31 +0.7 868,316 - glpF MCAODC_04270 0.36 +0.1 868,329 + glpF MCAODC_04270 0.38 +1.9 868,332 + glpF MCAODC_04270 0.38 -1.2 868,332 + glpF MCAODC_04270 0.38 +1.4 868,415 + glpF MCAODC_04270 0.48 +1.6 868,416 - glpF MCAODC_04270 0.48 +1.4 868,419 + glpF MCAODC_04270 0.49 +0.9 868,419 + glpF MCAODC_04270 0.49 +1.7 868,419 + glpF MCAODC_04270 0.49 -0.9 868,420 - glpF MCAODC_04270 0.49 +1.0 868,420 - glpF MCAODC_04270 0.49 -0.7
Or see this region's nucleotide sequence