Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hupA and yjaG are separated by 186 nucleotides yjaG and nfi are separated by 42 nucleotides nfi and hemE are separated by 9 nucleotides
MCAODC_03845: hupA - DNA-binding protein HU-alpha, at 776,774 to 777,046
hupA
MCAODC_03850: yjaG - Uncharacterized protein YjaG, at 777,233 to 777,823
yjaG
MCAODC_03855: nfi - deoxyribonuclease V, at 777,866 to 778,537
nfi
MCAODC_03860: hemE - uroporphyrinogen decarboxylase, at 778,547 to 779,611
hemE
Position (kb) 777
778
779 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 776.922 kb on - strand, within hupA at 776.984 kb on - strand, within hupA at 777.021 kb on - strand at 777.021 kb on - strand at 777.046 kb on + strand at 777.046 kb on + strand at 777.047 kb on - strand at 777.051 kb on + strand at 777.057 kb on + strand at 777.057 kb on + strand at 777.058 kb on - strand at 777.058 kb on - strand at 777.058 kb on - strand at 777.069 kb on - strand at 777.078 kb on + strand at 777.078 kb on + strand at 777.078 kb on + strand at 777.079 kb on - strand at 777.108 kb on + strand at 777.108 kb on + strand at 777.108 kb on + strand at 777.109 kb on - strand at 777.123 kb on + strand at 777.162 kb on + strand at 777.163 kb on - strand at 777.214 kb on + strand at 777.214 kb on + strand at 777.214 kb on + strand at 777.215 kb on - strand at 777.234 kb on + strand at 777.235 kb on - strand at 777.249 kb on - strand at 777.256 kb on + strand at 777.299 kb on + strand, within yjaG at 777.332 kb on + strand, within yjaG at 777.375 kb on - strand, within yjaG at 777.463 kb on + strand, within yjaG at 777.463 kb on + strand, within yjaG at 777.463 kb on + strand, within yjaG at 777.464 kb on - strand, within yjaG at 777.464 kb on - strand, within yjaG at 777.464 kb on - strand, within yjaG at 777.464 kb on - strand, within yjaG at 777.464 kb on - strand, within yjaG at 777.525 kb on - strand, within yjaG at 777.561 kb on + strand, within yjaG at 777.726 kb on + strand, within yjaG at 777.727 kb on - strand, within yjaG at 777.735 kb on + strand, within yjaG at 777.750 kb on - strand, within yjaG at 777.750 kb on - strand, within yjaG at 777.819 kb on - strand at 777.884 kb on - strand at 777.885 kb on + strand at 777.885 kb on + strand at 777.886 kb on - strand at 777.971 kb on + strand, within nfi at 777.971 kb on + strand, within nfi at 778.012 kb on + strand, within nfi at 778.018 kb on + strand, within nfi at 778.018 kb on + strand, within nfi at 778.018 kb on + strand, within nfi at 778.019 kb on - strand, within nfi at 778.022 kb on + strand, within nfi at 778.023 kb on - strand, within nfi at 778.075 kb on + strand, within nfi at 778.127 kb on - strand, within nfi at 778.133 kb on + strand, within nfi at 778.133 kb on + strand, within nfi at 778.134 kb on - strand, within nfi at 778.134 kb on - strand, within nfi at 778.241 kb on + strand, within nfi at 778.242 kb on - strand, within nfi at 778.242 kb on - strand, within nfi at 778.291 kb on - strand, within nfi at 778.320 kb on + strand, within nfi at 778.320 kb on + strand, within nfi at 778.320 kb on + strand, within nfi at 778.320 kb on + strand, within nfi at 778.320 kb on + strand, within nfi at 778.320 kb on + strand, within nfi at 778.321 kb on - strand, within nfi at 778.321 kb on - strand, within nfi at 778.321 kb on - strand, within nfi at 778.321 kb on - strand, within nfi at 778.351 kb on + strand, within nfi at 778.353 kb on + strand, within nfi at 778.520 kb on + strand at 778.521 kb on - strand at 778.558 kb on - strand at 778.558 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 776,922 - hupA MCAODC_03845 0.54 -0.3 776,984 - hupA MCAODC_03845 0.77 -1.2 777,021 - -0.7 777,021 - +0.4 777,046 + -1.0 777,046 + -2.0 777,047 - +0.3 777,051 + -2.7 777,057 + -0.8 777,057 + -0.8 777,058 - +0.0 777,058 - -2.0 777,058 - -0.6 777,069 - +0.2 777,078 + -0.3 777,078 + -0.4 777,078 + -0.6 777,079 - -0.9 777,108 + +1.5 777,108 + +0.0 777,108 + -0.0 777,109 - +0.4 777,123 + +0.1 777,162 + +0.3 777,163 - +0.3 777,214 + -0.7 777,214 + -1.6 777,214 + -0.4 777,215 - +1.4 777,234 + +0.7 777,235 - +1.2 777,249 - +0.6 777,256 + -0.3 777,299 + yjaG MCAODC_03850 0.11 -2.5 777,332 + yjaG MCAODC_03850 0.17 +0.1 777,375 - yjaG MCAODC_03850 0.24 +0.6 777,463 + yjaG MCAODC_03850 0.39 -0.8 777,463 + yjaG MCAODC_03850 0.39 -0.6 777,463 + yjaG MCAODC_03850 0.39 +0.6 777,464 - yjaG MCAODC_03850 0.39 +0.5 777,464 - yjaG MCAODC_03850 0.39 +1.0 777,464 - yjaG MCAODC_03850 0.39 -1.7 777,464 - yjaG MCAODC_03850 0.39 +0.6 777,464 - yjaG MCAODC_03850 0.39 -1.8 777,525 - yjaG MCAODC_03850 0.49 -0.3 777,561 + yjaG MCAODC_03850 0.55 -0.6 777,726 + yjaG MCAODC_03850 0.83 +0.4 777,727 - yjaG MCAODC_03850 0.84 +0.1 777,735 + yjaG MCAODC_03850 0.85 -0.6 777,750 - yjaG MCAODC_03850 0.87 -3.3 777,750 - yjaG MCAODC_03850 0.87 +0.2 777,819 - -0.7 777,884 - -0.2 777,885 + -1.7 777,885 + -0.5 777,886 - -2.0 777,971 + nfi MCAODC_03855 0.16 +0.7 777,971 + nfi MCAODC_03855 0.16 +0.8 778,012 + nfi MCAODC_03855 0.22 +0.7 778,018 + nfi MCAODC_03855 0.23 +0.3 778,018 + nfi MCAODC_03855 0.23 -0.9 778,018 + nfi MCAODC_03855 0.23 +0.9 778,019 - nfi MCAODC_03855 0.23 +0.3 778,022 + nfi MCAODC_03855 0.23 +1.0 778,023 - nfi MCAODC_03855 0.23 +0.0 778,075 + nfi MCAODC_03855 0.31 -0.0 778,127 - nfi MCAODC_03855 0.39 +0.4 778,133 + nfi MCAODC_03855 0.40 +1.2 778,133 + nfi MCAODC_03855 0.40 +0.1 778,134 - nfi MCAODC_03855 0.40 +0.9 778,134 - nfi MCAODC_03855 0.40 -0.1 778,241 + nfi MCAODC_03855 0.56 +0.0 778,242 - nfi MCAODC_03855 0.56 +1.1 778,242 - nfi MCAODC_03855 0.56 +0.3 778,291 - nfi MCAODC_03855 0.63 +0.7 778,320 + nfi MCAODC_03855 0.68 +0.9 778,320 + nfi MCAODC_03855 0.68 +1.7 778,320 + nfi MCAODC_03855 0.68 +0.5 778,320 + nfi MCAODC_03855 0.68 +0.6 778,320 + nfi MCAODC_03855 0.68 +0.3 778,320 + nfi MCAODC_03855 0.68 -0.5 778,321 - nfi MCAODC_03855 0.68 +1.3 778,321 - nfi MCAODC_03855 0.68 +1.0 778,321 - nfi MCAODC_03855 0.68 +0.6 778,321 - nfi MCAODC_03855 0.68 -0.7 778,351 + nfi MCAODC_03855 0.72 +0.6 778,353 + nfi MCAODC_03855 0.72 +1.4 778,520 + +0.3 778,521 - -1.6 778,558 - +1.0 778,558 - -2.0
Or see this region's nucleotide sequence