Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fimA and fimC are separated by 49 nucleotides fimC and fimD are separated by 12 nucleotides fimD and fimD are separated by 174 nucleotides fimD and MCAODC_01535 are separated by 12 nucleotides
MCAODC_01515: fimA - fimbrial protein, at 294,163 to 294,696
fimA
MCAODC_01520: fimC - molecular chaperone, at 294,746 to 295,429
fimC
MCAODC_01525: fimD - fimbrial assembly protein, at 295,442 to 297,343
fimD
MCAODC_01530: fimD - fimbrial assembly protein, at 297,518 to 297,892
fimD
MCAODC_01535: MCAODC_01535 - fimbrial family protein, at 297,905 to 298,864
_01535
Position (kb) 295
296
297
298 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 294.471 kb on + strand, within fimA at 294.579 kb on + strand, within fimA at 294.619 kb on + strand, within fimA at 294.660 kb on - strand at 294.660 kb on - strand at 294.678 kb on + strand at 294.757 kb on - strand at 295.340 kb on - strand, within fimC at 295.602 kb on - strand at 295.602 kb on - strand at 295.688 kb on - strand, within fimD at 295.723 kb on + strand, within fimD at 295.742 kb on - strand, within fimD at 295.781 kb on + strand, within fimD at 295.782 kb on - strand, within fimD at 295.832 kb on + strand, within fimD at 295.907 kb on + strand, within fimD at 295.908 kb on - strand, within fimD at 296.063 kb on - strand, within fimD at 296.219 kb on - strand, within fimD at 296.318 kb on - strand, within fimD at 296.370 kb on + strand, within fimD at 296.582 kb on + strand, within fimD at 296.708 kb on + strand, within fimD at 296.710 kb on + strand, within fimD at 296.711 kb on - strand, within fimD at 296.722 kb on - strand, within fimD at 297.013 kb on + strand, within fimD at 297.232 kb on - strand at 297.233 kb on + strand at 297.233 kb on + strand at 297.309 kb on + strand at 297.309 kb on + strand at 297.320 kb on + strand at 297.361 kb on + strand at 297.522 kb on + strand at 297.665 kb on + strand, within fimD at 297.666 kb on - strand, within fimD at 297.668 kb on - strand, within fimD at 297.669 kb on + strand, within fimD at 297.669 kb on + strand, within fimD at 297.880 kb on + strand at 298.001 kb on + strand, within MCAODC_01535 at 298.002 kb on - strand, within MCAODC_01535 at 298.003 kb on + strand, within MCAODC_01535 at 298.221 kb on + strand, within MCAODC_01535
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 294,471 + fimA MCAODC_01515 0.58 +0.7 294,579 + fimA MCAODC_01515 0.78 -0.2 294,619 + fimA MCAODC_01515 0.85 -0.3 294,660 - -1.5 294,660 - -1.6 294,678 + +0.4 294,757 - -1.1 295,340 - fimC MCAODC_01520 0.87 +0.7 295,602 - -1.9 295,602 - +0.2 295,688 - fimD MCAODC_01525 0.13 -1.6 295,723 + fimD MCAODC_01525 0.15 +0.6 295,742 - fimD MCAODC_01525 0.16 -0.0 295,781 + fimD MCAODC_01525 0.18 +2.0 295,782 - fimD MCAODC_01525 0.18 -0.5 295,832 + fimD MCAODC_01525 0.21 +0.1 295,907 + fimD MCAODC_01525 0.24 -0.0 295,908 - fimD MCAODC_01525 0.25 +0.7 296,063 - fimD MCAODC_01525 0.33 -0.1 296,219 - fimD MCAODC_01525 0.41 +2.4 296,318 - fimD MCAODC_01525 0.46 -1.9 296,370 + fimD MCAODC_01525 0.49 -2.0 296,582 + fimD MCAODC_01525 0.60 +0.2 296,708 + fimD MCAODC_01525 0.67 -0.9 296,710 + fimD MCAODC_01525 0.67 +1.0 296,711 - fimD MCAODC_01525 0.67 +1.3 296,722 - fimD MCAODC_01525 0.67 +1.1 297,013 + fimD MCAODC_01525 0.83 +0.1 297,232 - +0.4 297,233 + +0.3 297,233 + -3.5 297,309 + -0.3 297,309 + +0.0 297,320 + +0.0 297,361 + -1.1 297,522 + +1.1 297,665 + fimD MCAODC_01530 0.39 +0.2 297,666 - fimD MCAODC_01530 0.39 +2.0 297,668 - fimD MCAODC_01530 0.40 +1.8 297,669 + fimD MCAODC_01530 0.40 +0.3 297,669 + fimD MCAODC_01530 0.40 +0.7 297,880 + +0.7 298,001 + MCAODC_01535 0.10 +0.3 298,002 - MCAODC_01535 0.10 -0.4 298,003 + MCAODC_01535 0.10 +0.7 298,221 + MCAODC_01535 0.33 +1.5
Or see this region's nucleotide sequence