Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dkgB and Asp_trna are separated by 162 nucleotides Asp_trna and 5S_rrna are separated by 54 nucleotides 5S_rrna and 23S_rrna are separated by 94 nucleotides 23S_rrna and Ala_trna are separated by 183 nucleotides Ala_trna and Ile_trna are separated by 42 nucleotides Ile_trna and 16S_rrna are separated by 68 nucleotides
MCAODC_00520: dkgB - 2,5-didehydrogluconate reductase DkgB, at 83,909 to 84,712
dkgB
MCAODC_00525: Asp_trna - tRNA-Asp(gtc), at 84,875 to 84,951
_trna
MCAODC_00530: 5S_rrna - 5S ribosomal RNA, at 85,006 to 85,121
_rrna
MCAODC_00535: 23S_rrna - 23S ribosomal RNA, at 85,216 to 88,120
_rrna
MCAODC_00545: Ala_trna - tRNA-Ala(tgc), at 88,304 to 88,379
_trna
MCAODC_00550: Ile_trna - tRNA-Ile(gat), at 88,422 to 88,498
_trna
MCAODC_00555: 16S_rrna - 16S ribosomal RNA, at 88,567 to 90,108
_rrna
Position (kb) 85
86
87
88
89 Strain fitness (log2 ratio) -1
0
1
2
3 at 84.240 kb on + strand, within dkgB at 84.266 kb on - strand, within dkgB at 84.578 kb on + strand, within dkgB at 84.598 kb on - strand, within dkgB at 84.712 kb on + strand at 84.712 kb on + strand at 84.712 kb on + strand at 84.712 kb on + strand at 84.713 kb on - strand at 84.726 kb on + strand at 84.872 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 84,240 + dkgB MCAODC_00520 0.41 -0.0 84,266 - dkgB MCAODC_00520 0.44 +1.8 84,578 + dkgB MCAODC_00520 0.83 +1.7 84,598 - dkgB MCAODC_00520 0.86 +1.9 84,712 + -0.6 84,712 + +2.8 84,712 + -1.5 84,712 + -0.3 84,713 - +0.3 84,726 + +0.9 84,872 - +0.5
Or see this region's nucleotide sequence