Strain Fitness in Escherichia coli ECRC101 around MCAODC_00530

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdkgB and Asp_trna are separated by 162 nucleotidesAsp_trna and 5S_rrna are separated by 54 nucleotides5S_rrna and 23S_rrna are separated by 94 nucleotides MCAODC_00520: dkgB - 2,5-didehydrogluconate reductase DkgB, at 83,909 to 84,712 dkgB MCAODC_00525: Asp_trna - tRNA-Asp(gtc), at 84,875 to 84,951 _trna MCAODC_00530: 5S_rrna - 5S ribosomal RNA, at 85,006 to 85,121 _rrna MCAODC_00535: 23S_rrna - 23S ribosomal RNA, at 85,216 to 88,120 _rrna Position (kb) 85 86Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 84.016 kb on + strand, within dkgBat 84.022 kb on + strand, within dkgBat 84.044 kb on - strand, within dkgBat 84.044 kb on - strand, within dkgBat 84.059 kb on + strand, within dkgBat 84.059 kb on + strand, within dkgBat 84.059 kb on + strand, within dkgBat 84.060 kb on - strand, within dkgBat 84.180 kb on + strand, within dkgBat 84.180 kb on + strand, within dkgBat 84.180 kb on + strand, within dkgBat 84.181 kb on - strand, within dkgBat 84.181 kb on - strand, within dkgBat 84.181 kb on - strand, within dkgBat 84.188 kb on - strand, within dkgBat 84.188 kb on - strand, within dkgBat 84.191 kb on - strand, within dkgBat 84.240 kb on + strand, within dkgBat 84.266 kb on - strand, within dkgBat 84.578 kb on + strand, within dkgBat 84.598 kb on - strand, within dkgBat 84.712 kb on + strandat 84.712 kb on + strandat 84.712 kb on + strandat 84.712 kb on + strandat 84.713 kb on - strandat 84.726 kb on + strandat 84.872 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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84,016 + dkgB MCAODC_00520 0.13 +0.7
84,022 + dkgB MCAODC_00520 0.14 +0.3
84,044 - dkgB MCAODC_00520 0.17 -0.7
84,044 - dkgB MCAODC_00520 0.17 +1.1
84,059 + dkgB MCAODC_00520 0.19 -0.1
84,059 + dkgB MCAODC_00520 0.19 -1.1
84,059 + dkgB MCAODC_00520 0.19 +1.0
84,060 - dkgB MCAODC_00520 0.19 +1.6
84,180 + dkgB MCAODC_00520 0.34 +1.0
84,180 + dkgB MCAODC_00520 0.34 -0.4
84,180 + dkgB MCAODC_00520 0.34 +0.2
84,181 - dkgB MCAODC_00520 0.34 -2.4
84,181 - dkgB MCAODC_00520 0.34 -3.1
84,181 - dkgB MCAODC_00520 0.34 -0.0
84,188 - dkgB MCAODC_00520 0.35 +0.7
84,188 - dkgB MCAODC_00520 0.35 -0.8
84,191 - dkgB MCAODC_00520 0.35 -2.9
84,240 + dkgB MCAODC_00520 0.41 -0.0
84,266 - dkgB MCAODC_00520 0.44 +1.8
84,578 + dkgB MCAODC_00520 0.83 +1.7
84,598 - dkgB MCAODC_00520 0.86 +1.9
84,712 + -0.6
84,712 + +2.8
84,712 + -1.5
84,712 + -0.3
84,713 - +0.3
84,726 + +0.9
84,872 - +0.5

Or see this region's nucleotide sequence