Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ykfJ and yafP are separated by 306 nucleotides yafP and yafO are separated by 84 nucleotides yafO and yafN are separated by 2 nucleotides yafN and dinB are separated by 51 nucleotides
MCAODC_00230: ykfJ - Putative uncharacterized protein YkfJ, at 22,974 to 23,240
ykfJ
MCAODC_00235: yafP - GNAT family N-acetyltransferase, at 23,547 to 23,924
yafP
MCAODC_00240: yafO - type II toxin-antitoxin system mRNA interferase toxin YafO, at 24,009 to 24,407
yafO
MCAODC_00245: yafN - type I toxin-antitoxin system antitoxin YafN, at 24,410 to 24,703
yafN
MCAODC_00250: dinB - DNA polymerase IV, at 24,755 to 25,810
dinB
Position (kb) 24
25 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 23.085 kb on + strand, within ykfJ at 23.103 kb on + strand, within ykfJ at 23.140 kb on - strand, within ykfJ at 23.189 kb on + strand, within ykfJ at 23.190 kb on - strand, within ykfJ at 23.190 kb on - strand, within ykfJ at 23.194 kb on - strand, within ykfJ at 23.216 kb on + strand at 23.217 kb on - strand at 23.218 kb on + strand at 23.219 kb on - strand at 23.231 kb on - strand at 23.242 kb on + strand at 23.242 kb on + strand at 23.242 kb on + strand at 23.243 kb on - strand at 23.254 kb on - strand at 23.254 kb on - strand at 23.306 kb on - strand at 23.310 kb on - strand at 23.369 kb on + strand at 23.563 kb on + strand at 23.619 kb on - strand, within yafP at 23.619 kb on - strand, within yafP at 23.679 kb on - strand, within yafP at 23.696 kb on + strand, within yafP at 23.769 kb on - strand, within yafP at 23.769 kb on - strand, within yafP at 23.770 kb on + strand, within yafP at 23.950 kb on - strand at 23.975 kb on - strand at 24.051 kb on - strand, within yafO at 24.145 kb on + strand, within yafO at 24.146 kb on - strand, within yafO at 24.146 kb on - strand, within yafO at 24.228 kb on - strand, within yafO at 24.297 kb on + strand, within yafO at 24.297 kb on + strand, within yafO at 24.298 kb on - strand, within yafO at 24.298 kb on - strand, within yafO at 24.326 kb on - strand, within yafO at 24.419 kb on - strand at 24.703 kb on + strand at 24.703 kb on + strand at 24.719 kb on + strand at 24.743 kb on + strand at 24.744 kb on - strand at 24.746 kb on + strand at 24.747 kb on - strand at 24.749 kb on + strand at 24.749 kb on + strand at 24.751 kb on + strand at 24.751 kb on + strand at 24.875 kb on + strand, within dinB at 24.980 kb on + strand, within dinB at 24.980 kb on + strand, within dinB at 25.054 kb on + strand, within dinB at 25.136 kb on - strand, within dinB at 25.342 kb on + strand, within dinB
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 23,085 + ykfJ MCAODC_00230 0.42 +0.5 23,103 + ykfJ MCAODC_00230 0.48 +0.5 23,140 - ykfJ MCAODC_00230 0.62 -0.2 23,189 + ykfJ MCAODC_00230 0.81 +1.8 23,190 - ykfJ MCAODC_00230 0.81 +0.7 23,190 - ykfJ MCAODC_00230 0.81 -0.6 23,194 - ykfJ MCAODC_00230 0.82 +0.9 23,216 + +0.7 23,217 - -0.4 23,218 + -0.2 23,219 - -1.3 23,231 - +0.5 23,242 + -1.6 23,242 + +2.3 23,242 + +2.1 23,243 - +3.5 23,254 - +0.6 23,254 - -0.3 23,306 - +0.5 23,310 - -0.1 23,369 + +1.1 23,563 + -1.6 23,619 - yafP MCAODC_00235 0.19 -0.6 23,619 - yafP MCAODC_00235 0.19 +0.5 23,679 - yafP MCAODC_00235 0.35 -0.4 23,696 + yafP MCAODC_00235 0.39 +0.8 23,769 - yafP MCAODC_00235 0.59 +0.9 23,769 - yafP MCAODC_00235 0.59 -0.8 23,770 + yafP MCAODC_00235 0.59 +0.1 23,950 - +0.6 23,975 - -0.4 24,051 - yafO MCAODC_00240 0.11 -1.3 24,145 + yafO MCAODC_00240 0.34 -2.6 24,146 - yafO MCAODC_00240 0.34 +0.0 24,146 - yafO MCAODC_00240 0.34 -0.3 24,228 - yafO MCAODC_00240 0.55 +1.4 24,297 + yafO MCAODC_00240 0.72 +1.4 24,297 + yafO MCAODC_00240 0.72 -1.4 24,298 - yafO MCAODC_00240 0.72 +3.2 24,298 - yafO MCAODC_00240 0.72 -0.5 24,326 - yafO MCAODC_00240 0.79 +0.2 24,419 - -1.5 24,703 + -0.5 24,703 + -1.3 24,719 + -2.1 24,743 + -0.7 24,744 - +0.7 24,746 + -1.3 24,747 - +0.3 24,749 + +0.5 24,749 + -0.3 24,751 + +0.8 24,751 + +0.8 24,875 + dinB MCAODC_00250 0.11 -2.7 24,980 + dinB MCAODC_00250 0.21 -0.5 24,980 + dinB MCAODC_00250 0.21 +0.6 25,054 + dinB MCAODC_00250 0.28 +0.4 25,136 - dinB MCAODC_00250 0.36 -0.1 25,342 + dinB MCAODC_00250 0.56 +0.7
Or see this region's nucleotide sequence