Strain Fitness in Escherichia coli ECRC101 around MCAODC_00195

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntproA and proB are separated by 11 nucleotidesproB and phoE are separated by 287 nucleotides MCAODC_00190: proA - glutamate-5-semialdehyde dehydrogenase, at 14,727 to 15,980 proA MCAODC_00195: proB - Glutamate 5-kinase, at 15,992 to 17,095 proB MCAODC_00200: phoE - phosphoporin PhoE, at 17,383 to 18,438 phoE Position (kb) 15 16 17 18Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 15.056 kb on - strand, within proAat 15.129 kb on + strand, within proAat 15.129 kb on + strand, within proAat 15.180 kb on - strand, within proAat 15.180 kb on - strand, within proAat 15.181 kb on + strand, within proAat 15.213 kb on + strand, within proAat 15.219 kb on - strand, within proAat 15.219 kb on - strand, within proAat 15.219 kb on - strand, within proAat 15.221 kb on + strand, within proAat 15.221 kb on + strand, within proAat 15.221 kb on + strand, within proAat 15.221 kb on + strand, within proAat 15.221 kb on + strand, within proAat 15.222 kb on - strand, within proAat 15.222 kb on - strand, within proAat 15.222 kb on - strand, within proAat 15.222 kb on - strand, within proAat 15.222 kb on - strand, within proAat 15.222 kb on - strand, within proAat 15.222 kb on - strand, within proAat 15.222 kb on - strand, within proAat 15.222 kb on - strand, within proAat 15.222 kb on - strand, within proAat 15.259 kb on - strand, within proAat 15.298 kb on + strand, within proAat 15.302 kb on + strand, within proAat 15.302 kb on + strand, within proAat 15.303 kb on - strand, within proAat 15.310 kb on - strand, within proAat 15.403 kb on + strand, within proAat 15.403 kb on + strand, within proAat 15.404 kb on - strand, within proAat 15.404 kb on - strand, within proAat 15.404 kb on - strand, within proAat 15.414 kb on - strand, within proAat 15.450 kb on - strand, within proAat 15.625 kb on + strand, within proAat 15.625 kb on + strand, within proAat 15.688 kb on - strand, within proAat 15.741 kb on + strand, within proAat 15.741 kb on + strand, within proAat 15.741 kb on + strand, within proAat 15.742 kb on - strand, within proAat 15.742 kb on - strand, within proAat 15.742 kb on - strand, within proAat 15.939 kb on - strandat 15.939 kb on - strandat 15.939 kb on - strandat 16.042 kb on - strandat 16.050 kb on - strandat 16.085 kb on - strandat 16.091 kb on + strandat 16.147 kb on + strand, within proBat 16.148 kb on - strand, within proBat 16.196 kb on - strand, within proBat 16.257 kb on - strand, within proBat 16.572 kb on + strand, within proBat 16.573 kb on - strand, within proBat 16.573 kb on - strand, within proBat 16.701 kb on - strand, within proBat 16.816 kb on - strand, within proBat 16.957 kb on + strand, within proBat 16.958 kb on - strand, within proBat 16.982 kb on + strand, within proBat 16.982 kb on + strand, within proBat 16.982 kb on + strand, within proBat 16.983 kb on - strand, within proBat 17.070 kb on - strandat 17.218 kb on - strandat 17.259 kb on - strandat 17.274 kb on + strandat 17.362 kb on + strandat 17.453 kb on + strandat 17.454 kb on - strandat 17.509 kb on + strand, within phoEat 17.529 kb on + strand, within phoEat 17.549 kb on - strand, within phoEat 17.692 kb on + strand, within phoEat 17.740 kb on + strand, within phoEat 17.740 kb on + strand, within phoEat 17.740 kb on + strand, within phoEat 17.740 kb on + strand, within phoEat 17.740 kb on + strand, within phoEat 17.741 kb on - strand, within phoEat 17.741 kb on - strand, within phoEat 17.741 kb on - strand, within phoEat 17.763 kb on + strand, within phoEat 17.763 kb on + strand, within phoEat 17.764 kb on - strand, within phoEat 17.843 kb on - strand, within phoEat 17.965 kb on + strand, within phoEat 17.965 kb on + strand, within phoEat 17.966 kb on - strand, within phoEat 17.995 kb on - strand, within phoEat 18.006 kb on + strand, within phoEat 18.007 kb on - strand, within phoEat 18.007 kb on - strand, within phoEat 18.092 kb on - strand, within phoE

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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15,056 - proA MCAODC_00190 0.26 -4.1
15,129 + proA MCAODC_00190 0.32 -0.8
15,129 + proA MCAODC_00190 0.32 -0.7
15,180 - proA MCAODC_00190 0.36 -4.3
15,180 - proA MCAODC_00190 0.36 -3.1
15,181 + proA MCAODC_00190 0.36 -3.9
15,213 + proA MCAODC_00190 0.39 -2.8
15,219 - proA MCAODC_00190 0.39 -4.3
15,219 - proA MCAODC_00190 0.39 -3.5
15,219 - proA MCAODC_00190 0.39 -0.5
15,221 + proA MCAODC_00190 0.39 -3.4
15,221 + proA MCAODC_00190 0.39 -4.0
15,221 + proA MCAODC_00190 0.39 -1.4
15,221 + proA MCAODC_00190 0.39 -4.2
15,221 + proA MCAODC_00190 0.39 -1.5
15,222 - proA MCAODC_00190 0.39 -2.9
15,222 - proA MCAODC_00190 0.39 -2.0
15,222 - proA MCAODC_00190 0.39 -2.2
15,222 - proA MCAODC_00190 0.39 -2.4
15,222 - proA MCAODC_00190 0.39 -4.4
15,222 - proA MCAODC_00190 0.39 -1.9
15,222 - proA MCAODC_00190 0.39 -3.2
15,222 - proA MCAODC_00190 0.39 -2.1
15,222 - proA MCAODC_00190 0.39 -0.3
15,222 - proA MCAODC_00190 0.39 -5.0
15,259 - proA MCAODC_00190 0.42 -0.9
15,298 + proA MCAODC_00190 0.46 -2.1
15,302 + proA MCAODC_00190 0.46 +0.7
15,302 + proA MCAODC_00190 0.46 -2.3
15,303 - proA MCAODC_00190 0.46 -2.7
15,310 - proA MCAODC_00190 0.46 -4.1
15,403 + proA MCAODC_00190 0.54 -0.1
15,403 + proA MCAODC_00190 0.54 -3.2
15,404 - proA MCAODC_00190 0.54 -2.3
15,404 - proA MCAODC_00190 0.54 -4.7
15,404 - proA MCAODC_00190 0.54 -3.2
15,414 - proA MCAODC_00190 0.55 -3.7
15,450 - proA MCAODC_00190 0.58 -3.4
15,625 + proA MCAODC_00190 0.72 -0.1
15,625 + proA MCAODC_00190 0.72 -2.8
15,688 - proA MCAODC_00190 0.77 -0.9
15,741 + proA MCAODC_00190 0.81 -1.4
15,741 + proA MCAODC_00190 0.81 -5.2
15,741 + proA MCAODC_00190 0.81 -4.1
15,742 - proA MCAODC_00190 0.81 -2.1
15,742 - proA MCAODC_00190 0.81 -1.7
15,742 - proA MCAODC_00190 0.81 -2.9
15,939 - -5.1
15,939 - -4.4
15,939 - -3.4
16,042 - -3.2
16,050 - -4.1
16,085 - -1.2
16,091 + -2.4
16,147 + proB MCAODC_00195 0.14 -3.0
16,148 - proB MCAODC_00195 0.14 -0.9
16,196 - proB MCAODC_00195 0.18 -4.0
16,257 - proB MCAODC_00195 0.24 -1.5
16,572 + proB MCAODC_00195 0.53 -4.4
16,573 - proB MCAODC_00195 0.53 -2.6
16,573 - proB MCAODC_00195 0.53 -4.3
16,701 - proB MCAODC_00195 0.64 -1.4
16,816 - proB MCAODC_00195 0.75 -3.2
16,957 + proB MCAODC_00195 0.87 -2.5
16,958 - proB MCAODC_00195 0.88 -1.0
16,982 + proB MCAODC_00195 0.90 -2.8
16,982 + proB MCAODC_00195 0.90 -3.3
16,982 + proB MCAODC_00195 0.90 -3.1
16,983 - proB MCAODC_00195 0.90 -3.4
17,070 - -4.3
17,218 - -1.2
17,259 - -2.1
17,274 + -0.5
17,362 + -0.7
17,453 + +0.3
17,454 - -0.5
17,509 + phoE MCAODC_00200 0.12 -0.0
17,529 + phoE MCAODC_00200 0.14 -0.9
17,549 - phoE MCAODC_00200 0.16 +0.3
17,692 + phoE MCAODC_00200 0.29 +0.5
17,740 + phoE MCAODC_00200 0.34 -2.4
17,740 + phoE MCAODC_00200 0.34 -1.1
17,740 + phoE MCAODC_00200 0.34 -0.9
17,740 + phoE MCAODC_00200 0.34 +1.8
17,740 + phoE MCAODC_00200 0.34 +0.6
17,741 - phoE MCAODC_00200 0.34 +0.4
17,741 - phoE MCAODC_00200 0.34 +2.2
17,741 - phoE MCAODC_00200 0.34 -1.1
17,763 + phoE MCAODC_00200 0.36 -1.0
17,763 + phoE MCAODC_00200 0.36 +1.1
17,764 - phoE MCAODC_00200 0.36 -0.1
17,843 - phoE MCAODC_00200 0.44 +0.5
17,965 + phoE MCAODC_00200 0.55 +0.3
17,965 + phoE MCAODC_00200 0.55 -0.4
17,966 - phoE MCAODC_00200 0.55 +0.6
17,995 - phoE MCAODC_00200 0.58 -0.3
18,006 + phoE MCAODC_00200 0.59 +0.9
18,007 - phoE MCAODC_00200 0.59 +1.4
18,007 - phoE MCAODC_00200 0.59 -0.4
18,092 - phoE MCAODC_00200 0.67 +2.3

Or see this region's nucleotide sequence