Experiment: Cytidine 0.41115 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS15220 and A4249_RS15225 are separated by 299 nucleotides A4249_RS15225 and A4249_RS15230 overlap by 1 nucleotides A4249_RS15230 and A4249_RS15235 overlap by 8 nucleotides
A4249_RS15220: A4249_RS15220 - GTP cyclohydrolase I FolE, at 3,080,861 to 3,081,445
_RS15220
A4249_RS15225: A4249_RS15225 - flagellar biosynthesis protein FliQ, at 3,081,745 to 3,082,008
_RS15225
A4249_RS15230: A4249_RS15230 - flagellar biosynthetic protein FliR, at 3,082,008 to 3,082,781
_RS15230
A4249_RS15235: A4249_RS15235 - flagellar biosynthesis protein FlhB, at 3,082,774 to 3,083,850
_RS15235
Position (kb)
3082
3083 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3081.454 kb on - strand at 3081.457 kb on + strand at 3081.500 kb on + strand at 3081.501 kb on - strand at 3081.501 kb on - strand at 3081.547 kb on + strand at 3081.547 kb on + strand at 3081.547 kb on + strand at 3081.548 kb on - strand at 3081.548 kb on - strand at 3081.548 kb on - strand at 3081.556 kb on + strand at 3081.556 kb on + strand at 3081.557 kb on - strand at 3081.697 kb on - strand at 3081.726 kb on - strand at 3081.726 kb on - strand at 3081.895 kb on + strand, within A4249_RS15225 at 3081.895 kb on + strand, within A4249_RS15225 at 3081.895 kb on + strand, within A4249_RS15225 at 3081.896 kb on - strand, within A4249_RS15225 at 3081.896 kb on - strand, within A4249_RS15225 at 3081.896 kb on - strand, within A4249_RS15225 at 3082.006 kb on + strand at 3082.006 kb on + strand at 3082.006 kb on + strand at 3082.006 kb on + strand at 3082.006 kb on + strand at 3082.006 kb on + strand at 3082.006 kb on + strand at 3082.006 kb on + strand at 3082.006 kb on + strand at 3082.006 kb on + strand at 3082.006 kb on + strand at 3082.007 kb on - strand at 3082.007 kb on - strand at 3082.007 kb on - strand at 3082.007 kb on - strand at 3082.007 kb on - strand at 3082.007 kb on - strand at 3082.007 kb on - strand at 3082.007 kb on - strand at 3082.007 kb on - strand at 3082.007 kb on - strand at 3082.007 kb on - strand at 3082.017 kb on + strand at 3082.018 kb on - strand at 3082.107 kb on + strand, within A4249_RS15230 at 3082.107 kb on + strand, within A4249_RS15230 at 3082.107 kb on + strand, within A4249_RS15230 at 3082.107 kb on + strand, within A4249_RS15230 at 3082.107 kb on + strand, within A4249_RS15230 at 3082.107 kb on + strand, within A4249_RS15230 at 3082.107 kb on + strand, within A4249_RS15230 at 3082.107 kb on + strand, within A4249_RS15230 at 3082.107 kb on + strand, within A4249_RS15230 at 3082.107 kb on + strand, within A4249_RS15230 at 3082.107 kb on + strand, within A4249_RS15230 at 3082.108 kb on - strand, within A4249_RS15230 at 3082.108 kb on - strand, within A4249_RS15230 at 3082.108 kb on - strand, within A4249_RS15230 at 3082.108 kb on - strand, within A4249_RS15230 at 3082.108 kb on - strand, within A4249_RS15230 at 3082.108 kb on - strand, within A4249_RS15230 at 3082.108 kb on - strand, within A4249_RS15230 at 3082.108 kb on - strand, within A4249_RS15230 at 3082.544 kb on + strand, within A4249_RS15230 at 3082.544 kb on + strand, within A4249_RS15230 at 3082.544 kb on + strand, within A4249_RS15230 at 3082.544 kb on + strand, within A4249_RS15230 at 3082.545 kb on - strand, within A4249_RS15230 at 3082.545 kb on - strand, within A4249_RS15230 at 3082.545 kb on - strand, within A4249_RS15230 at 3082.545 kb on - strand, within A4249_RS15230 at 3082.545 kb on - strand, within A4249_RS15230 at 3082.545 kb on - strand, within A4249_RS15230 at 3082.545 kb on - strand, within A4249_RS15230 at 3082.545 kb on - strand, within A4249_RS15230 at 3082.545 kb on - strand, within A4249_RS15230 at 3082.645 kb on - strand, within A4249_RS15230 at 3082.752 kb on + strand at 3082.752 kb on + strand at 3083.600 kb on - strand, within A4249_RS15235 at 3083.600 kb on - strand, within A4249_RS15235 at 3083.617 kb on + strand, within A4249_RS15235 at 3083.618 kb on - strand, within A4249_RS15235 at 3083.752 kb on + strand at 3083.778 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cytidine 0.41115 mM (C) remove 3,081,454 - +0.3 3,081,457 + +0.3 3,081,500 + +0.9 3,081,501 - -0.1 3,081,501 - +1.3 3,081,547 + +1.3 3,081,547 + +0.8 3,081,547 + +0.6 3,081,548 - -1.5 3,081,548 - -0.2 3,081,548 - +1.0 3,081,556 + -0.6 3,081,556 + -0.1 3,081,557 - -0.3 3,081,697 - +2.8 3,081,726 - +0.6 3,081,726 - -0.7 3,081,895 + A4249_RS15225 0.57 -0.8 3,081,895 + A4249_RS15225 0.57 +2.3 3,081,895 + A4249_RS15225 0.57 +0.1 3,081,896 - A4249_RS15225 0.57 +0.1 3,081,896 - A4249_RS15225 0.57 +1.1 3,081,896 - A4249_RS15225 0.57 +0.1 3,082,006 + -1.8 3,082,006 + -0.7 3,082,006 + -0.8 3,082,006 + +0.7 3,082,006 + -0.1 3,082,006 + -0.3 3,082,006 + -0.2 3,082,006 + +0.3 3,082,006 + +0.2 3,082,006 + -0.6 3,082,006 + +0.5 3,082,007 - -2.0 3,082,007 - +1.1 3,082,007 - -1.3 3,082,007 - +0.5 3,082,007 - -0.8 3,082,007 - +0.3 3,082,007 - -0.1 3,082,007 - -0.5 3,082,007 - +1.0 3,082,007 - +0.6 3,082,007 - -1.1 3,082,017 + +0.2 3,082,018 - +0.1 3,082,107 + A4249_RS15230 0.13 +0.5 3,082,107 + A4249_RS15230 0.13 +1.2 3,082,107 + A4249_RS15230 0.13 -0.7 3,082,107 + A4249_RS15230 0.13 +1.1 3,082,107 + A4249_RS15230 0.13 -0.4 3,082,107 + A4249_RS15230 0.13 -0.3 3,082,107 + A4249_RS15230 0.13 +1.0 3,082,107 + A4249_RS15230 0.13 +0.8 3,082,107 + A4249_RS15230 0.13 +0.9 3,082,107 + A4249_RS15230 0.13 +0.3 3,082,107 + A4249_RS15230 0.13 -0.6 3,082,108 - A4249_RS15230 0.13 +0.8 3,082,108 - A4249_RS15230 0.13 -0.7 3,082,108 - A4249_RS15230 0.13 -0.6 3,082,108 - A4249_RS15230 0.13 +0.5 3,082,108 - A4249_RS15230 0.13 +0.3 3,082,108 - A4249_RS15230 0.13 +1.3 3,082,108 - A4249_RS15230 0.13 +1.3 3,082,108 - A4249_RS15230 0.13 -0.8 3,082,544 + A4249_RS15230 0.69 -0.3 3,082,544 + A4249_RS15230 0.69 +1.2 3,082,544 + A4249_RS15230 0.69 +0.7 3,082,544 + A4249_RS15230 0.69 +0.3 3,082,545 - A4249_RS15230 0.69 -0.3 3,082,545 - A4249_RS15230 0.69 +0.9 3,082,545 - A4249_RS15230 0.69 -0.4 3,082,545 - A4249_RS15230 0.69 -0.2 3,082,545 - A4249_RS15230 0.69 +1.3 3,082,545 - A4249_RS15230 0.69 -1.0 3,082,545 - A4249_RS15230 0.69 +0.5 3,082,545 - A4249_RS15230 0.69 -0.5 3,082,545 - A4249_RS15230 0.69 +0.7 3,082,645 - A4249_RS15230 0.82 +0.5 3,082,752 + -1.8 3,082,752 + -0.9 3,083,600 - A4249_RS15235 0.77 +2.5 3,083,600 - A4249_RS15235 0.77 +0.1 3,083,617 + A4249_RS15235 0.78 +0.2 3,083,618 - A4249_RS15235 0.78 +0.9 3,083,752 + -2.1 3,083,778 + +0.3
Or see this region's nucleotide sequence