Experiment: Cytidine 0.41115 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS05495 and A4249_RS05500 are separated by 84 nucleotides A4249_RS05500 and A4249_RS05505 are separated by 134 nucleotides A4249_RS05505 and A4249_RS05510 overlap by 4 nucleotides A4249_RS05510 and A4249_RS05515 are separated by 50 nucleotides
A4249_RS05495: A4249_RS05495 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 1,122,667 to 1,123,230
_RS05495
A4249_RS05500: A4249_RS05500 - DNA-directed RNA polymerase subunit omega, at 1,123,315 to 1,123,674
_RS05500
A4249_RS05505: A4249_RS05505 - bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 1,123,809 to 1,125,968
_RS05505
A4249_RS05510: A4249_RS05510 - TIGR00730 family Rossman fold protein, at 1,125,965 to 1,126,723
_RS05510
A4249_RS05515: A4249_RS05515 - orotate phosphoribosyltransferase, at 1,126,774 to 1,127,358
_RS05515
Position (kb)
1123
1124
1125
1126 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1123.700 kb on + strand at 1123.848 kb on + strand at 1123.848 kb on + strand at 1123.848 kb on + strand at 1123.848 kb on + strand at 1123.887 kb on + strand at 1123.887 kb on + strand at 1123.887 kb on + strand at 1123.887 kb on + strand at 1123.887 kb on + strand at 1123.887 kb on + strand at 1123.893 kb on + strand at 1123.893 kb on + strand at 1123.893 kb on + strand at 1123.893 kb on + strand at 1123.894 kb on - strand at 1123.983 kb on + strand at 1123.983 kb on + strand at 1123.985 kb on + strand at 1124.237 kb on + strand, within A4249_RS05505 at 1124.304 kb on + strand, within A4249_RS05505 at 1124.977 kb on - strand, within A4249_RS05505 at 1124.977 kb on - strand, within A4249_RS05505 at 1125.057 kb on + strand, within A4249_RS05505 at 1125.057 kb on + strand, within A4249_RS05505 at 1125.058 kb on - strand, within A4249_RS05505 at 1125.058 kb on - strand, within A4249_RS05505 at 1125.245 kb on + strand, within A4249_RS05505 at 1125.245 kb on + strand, within A4249_RS05505 at 1125.553 kb on + strand, within A4249_RS05505 at 1125.554 kb on - strand, within A4249_RS05505 at 1125.740 kb on + strand, within A4249_RS05505 at 1126.085 kb on + strand, within A4249_RS05510 at 1126.085 kb on + strand, within A4249_RS05510 at 1126.085 kb on + strand, within A4249_RS05510 at 1126.085 kb on + strand, within A4249_RS05510 at 1126.085 kb on + strand, within A4249_RS05510 at 1126.085 kb on + strand, within A4249_RS05510 at 1126.086 kb on - strand, within A4249_RS05510 at 1126.086 kb on - strand, within A4249_RS05510 at 1126.178 kb on + strand, within A4249_RS05510 at 1126.178 kb on + strand, within A4249_RS05510 at 1126.178 kb on + strand, within A4249_RS05510 at 1126.179 kb on - strand, within A4249_RS05510 at 1126.179 kb on - strand, within A4249_RS05510 at 1126.382 kb on + strand, within A4249_RS05510 at 1126.382 kb on + strand, within A4249_RS05510 at 1126.415 kb on + strand, within A4249_RS05510 at 1126.415 kb on + strand, within A4249_RS05510 at 1126.415 kb on + strand, within A4249_RS05510 at 1126.415 kb on + strand, within A4249_RS05510 at 1126.415 kb on + strand, within A4249_RS05510 at 1126.416 kb on - strand, within A4249_RS05510 at 1126.416 kb on - strand, within A4249_RS05510 at 1126.416 kb on - strand, within A4249_RS05510 at 1126.571 kb on + strand, within A4249_RS05510 at 1126.571 kb on + strand, within A4249_RS05510 at 1126.572 kb on - strand, within A4249_RS05510 at 1126.762 kb on + strand at 1126.762 kb on + strand at 1126.763 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cytidine 0.41115 mM (C) remove 1,123,700 + -0.6 1,123,848 + -1.3 1,123,848 + -0.1 1,123,848 + -1.2 1,123,848 + +1.2 1,123,887 + +1.0 1,123,887 + +0.8 1,123,887 + -0.1 1,123,887 + -0.1 1,123,887 + -1.8 1,123,887 + +0.2 1,123,893 + -0.0 1,123,893 + +0.6 1,123,893 + -0.7 1,123,893 + +0.9 1,123,894 - -0.7 1,123,983 + +0.2 1,123,983 + -0.9 1,123,985 + +0.4 1,124,237 + A4249_RS05505 0.20 +2.4 1,124,304 + A4249_RS05505 0.23 +0.4 1,124,977 - A4249_RS05505 0.54 +0.4 1,124,977 - A4249_RS05505 0.54 +0.4 1,125,057 + A4249_RS05505 0.58 -0.1 1,125,057 + A4249_RS05505 0.58 -0.2 1,125,058 - A4249_RS05505 0.58 -1.1 1,125,058 - A4249_RS05505 0.58 +1.4 1,125,245 + A4249_RS05505 0.66 -0.1 1,125,245 + A4249_RS05505 0.66 -0.2 1,125,553 + A4249_RS05505 0.81 -1.1 1,125,554 - A4249_RS05505 0.81 -0.1 1,125,740 + A4249_RS05505 0.89 +0.6 1,126,085 + A4249_RS05510 0.16 -0.3 1,126,085 + A4249_RS05510 0.16 +1.0 1,126,085 + A4249_RS05510 0.16 -0.2 1,126,085 + A4249_RS05510 0.16 +1.0 1,126,085 + A4249_RS05510 0.16 +0.1 1,126,085 + A4249_RS05510 0.16 +0.0 1,126,086 - A4249_RS05510 0.16 -0.5 1,126,086 - A4249_RS05510 0.16 -0.8 1,126,178 + A4249_RS05510 0.28 -1.0 1,126,178 + A4249_RS05510 0.28 +0.1 1,126,178 + A4249_RS05510 0.28 +1.0 1,126,179 - A4249_RS05510 0.28 -0.3 1,126,179 - A4249_RS05510 0.28 +0.4 1,126,382 + A4249_RS05510 0.55 +0.4 1,126,382 + A4249_RS05510 0.55 +1.3 1,126,415 + A4249_RS05510 0.59 +0.0 1,126,415 + A4249_RS05510 0.59 -1.4 1,126,415 + A4249_RS05510 0.59 +0.6 1,126,415 + A4249_RS05510 0.59 -0.1 1,126,415 + A4249_RS05510 0.59 +0.1 1,126,416 - A4249_RS05510 0.59 -0.4 1,126,416 - A4249_RS05510 0.59 +1.0 1,126,416 - A4249_RS05510 0.59 -1.1 1,126,571 + A4249_RS05510 0.80 +2.0 1,126,571 + A4249_RS05510 0.80 -0.2 1,126,572 - A4249_RS05510 0.80 -0.1 1,126,762 + +0.3 1,126,762 + -1.3 1,126,763 - +0.7
Or see this region's nucleotide sequence