Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt narK and narG are separated by 392 nucleotides narG and narH overlap by 4 nucleotides
OKFHMN_28535: narK - nitrate transporter NarK, at 5,471,920 to 5,473,311
narK
OKFHMN_28540: narG - nitrate reductase subunit alpha, at 5,473,704 to 5,477,447
narG
OKFHMN_28545: narH - nitrate reductase subunit beta, at 5,477,444 to 5,478,982
narH
Position (kb) 5473
5474
5475
5476
5477
5478 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5472.797 kb on - strand, within narK at 5472.852 kb on - strand, within narK at 5473.286 kb on + strand at 5473.431 kb on + strand at 5474.117 kb on + strand, within narG at 5474.150 kb on + strand, within narG at 5474.437 kb on - strand, within narG at 5474.873 kb on + strand, within narG at 5475.321 kb on - strand, within narG at 5475.435 kb on + strand, within narG at 5475.439 kb on + strand, within narG at 5475.542 kb on + strand, within narG at 5475.848 kb on + strand, within narG at 5475.895 kb on - strand, within narG at 5476.100 kb on + strand, within narG at 5476.392 kb on + strand, within narG at 5476.653 kb on - strand, within narG at 5476.887 kb on + strand, within narG at 5477.252 kb on + strand at 5477.273 kb on + strand at 5477.330 kb on + strand at 5477.330 kb on + strand at 5477.331 kb on - strand at 5477.334 kb on + strand at 5477.337 kb on + strand at 5477.354 kb on + strand at 5477.374 kb on + strand at 5477.978 kb on + strand, within narH at 5478.048 kb on - strand, within narH at 5478.241 kb on + strand, within narH at 5478.391 kb on + strand, within narH at 5478.391 kb on + strand, within narH
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 5,472,797 - narK OKFHMN_28535 0.63 +0.9 5,472,852 - narK OKFHMN_28535 0.67 +0.7 5,473,286 + -0.1 5,473,431 + -0.4 5,474,117 + narG OKFHMN_28540 0.11 -0.1 5,474,150 + narG OKFHMN_28540 0.12 +0.1 5,474,437 - narG OKFHMN_28540 0.20 -1.0 5,474,873 + narG OKFHMN_28540 0.31 -0.7 5,475,321 - narG OKFHMN_28540 0.43 +0.3 5,475,435 + narG OKFHMN_28540 0.46 +1.3 5,475,439 + narG OKFHMN_28540 0.46 -0.4 5,475,542 + narG OKFHMN_28540 0.49 -1.1 5,475,848 + narG OKFHMN_28540 0.57 +0.9 5,475,895 - narG OKFHMN_28540 0.59 -2.8 5,476,100 + narG OKFHMN_28540 0.64 -1.5 5,476,392 + narG OKFHMN_28540 0.72 +0.2 5,476,653 - narG OKFHMN_28540 0.79 +0.0 5,476,887 + narG OKFHMN_28540 0.85 -0.0 5,477,252 + +2.1 5,477,273 + -0.2 5,477,330 + +0.7 5,477,330 + +1.5 5,477,331 - +1.3 5,477,334 + +1.0 5,477,337 + +0.4 5,477,354 + +0.3 5,477,374 + -0.6 5,477,978 + narH OKFHMN_28545 0.35 -0.9 5,478,048 - narH OKFHMN_28545 0.39 +0.6 5,478,241 + narH OKFHMN_28545 0.52 -0.3 5,478,391 + narH OKFHMN_28545 0.62 +0.1 5,478,391 + narH OKFHMN_28545 0.62 -1.3
Or see this region's nucleotide sequence