Strain Fitness in Escherichia coli ECRC100 around OKFHMN_28295

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdadA and dadX are separated by 9 nucleotidesdadX and tnp-IS629 are separated by 337 nucleotidestnp-IS629 and tnp-IS3 overlap by 1 nucleotides OKFHMN_28290: dadA - D-amino acid dehydrogenase, at 5,426,392 to 5,427,690 dadA OKFHMN_28295: dadX - catabolic alanine racemase DadX, at 5,427,700 to 5,428,770 dadX OKFHMN_28300: tnp-IS629 - IS3 family IS629 transposase, at 5,429,108 to 5,429,434 tnp-IS629 OKFHMN_28305: tnp-IS3 - IS3 family transposase, at 5,429,434 to 5,430,321 tnp-IS3 Position (kb) 5427 5428 5429Strain fitness (log2 ratio) -2 -1 0 1at 5426.779 kb on + strand, within dadAat 5426.779 kb on + strand, within dadAat 5427.040 kb on - strand, within dadAat 5427.872 kb on + strand, within dadXat 5427.999 kb on - strand, within dadXat 5428.404 kb on - strand, within dadXat 5428.471 kb on - strand, within dadX

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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5,426,779 + dadA OKFHMN_28290 0.30 -2.0
5,426,779 + dadA OKFHMN_28290 0.30 +1.5
5,427,040 - dadA OKFHMN_28290 0.50 +0.7
5,427,872 + dadX OKFHMN_28295 0.16 -0.8
5,427,999 - dadX OKFHMN_28295 0.28 -0.6
5,428,404 - dadX OKFHMN_28295 0.66 +0.7
5,428,471 - dadX OKFHMN_28295 0.72 -0.3

Or see this region's nucleotide sequence