Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gdhA and ynjH are separated by 235 nucleotides ynjH and nudG overlap by 35 nucleotides nudG and ynjF are separated by 86 nucleotides ynjF and ynjE are separated by 8 nucleotides
OKFHMN_26840: gdhA - NADP-specific glutamate dehydrogenase, at 5,167,193 to 5,168,536
gdhA
OKFHMN_26845: ynjH - Uncharacterized protein YnjH, at 5,168,772 to 5,169,044
ynjH
OKFHMN_26850: nudG - CTP pyrophosphohydrolase, at 5,169,010 to 5,169,417
nudG
OKFHMN_26855: ynjF - Inner membrane protein YnjF, at 5,169,504 to 5,170,124
ynjF
OKFHMN_26860: ynjE - thiosulfate sulfurtransferase YnjE, at 5,170,133 to 5,171,440
ynjE
Position (kb) 5169
5170 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5168.177 kb on - strand, within gdhA at 5168.219 kb on - strand, within gdhA at 5168.219 kb on - strand, within gdhA at 5168.445 kb on - strand at 5168.541 kb on - strand at 5168.541 kb on - strand at 5168.611 kb on - strand at 5168.612 kb on + strand at 5168.613 kb on - strand at 5168.665 kb on + strand at 5168.677 kb on + strand at 5168.678 kb on - strand at 5168.699 kb on - strand at 5168.800 kb on + strand, within ynjH at 5168.812 kb on + strand, within ynjH at 5168.835 kb on + strand, within ynjH at 5169.052 kb on + strand, within nudG at 5169.222 kb on - strand, within nudG at 5169.222 kb on - strand, within nudG at 5169.486 kb on + strand at 5169.488 kb on + strand at 5170.009 kb on + strand, within ynjF at 5170.251 kb on + strand at 5170.336 kb on + strand, within ynjE
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 5,168,177 - gdhA OKFHMN_26840 0.73 -1.1 5,168,219 - gdhA OKFHMN_26840 0.76 -0.1 5,168,219 - gdhA OKFHMN_26840 0.76 +0.0 5,168,445 - -0.3 5,168,541 - +1.8 5,168,541 - +1.5 5,168,611 - +0.7 5,168,612 + -0.4 5,168,613 - -0.3 5,168,665 + -0.4 5,168,677 + +1.6 5,168,678 - +0.2 5,168,699 - +1.9 5,168,800 + ynjH OKFHMN_26845 0.10 -0.4 5,168,812 + ynjH OKFHMN_26845 0.15 -0.0 5,168,835 + ynjH OKFHMN_26845 0.23 +0.6 5,169,052 + nudG OKFHMN_26850 0.10 -0.9 5,169,222 - nudG OKFHMN_26850 0.52 -0.4 5,169,222 - nudG OKFHMN_26850 0.52 -2.8 5,169,486 + +0.1 5,169,488 + +0.5 5,170,009 + ynjF OKFHMN_26855 0.81 +0.6 5,170,251 + +0.8 5,170,336 + ynjE OKFHMN_26860 0.16 -0.7
Or see this region's nucleotide sequence