Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ansA and sIMPL are separated by 131 nucleotides sIMPL and sppA are separated by 115 nucleotides sppA and ydjA are separated by 160 nucleotides ydjA and selD are separated by 116 nucleotides
OKFHMN_26805: ansA - asparaginase, at 5,158,240 to 5,159,256
ansA
OKFHMN_26810: sIMPL - SIMPL domain-containing protein, at 5,159,388 to 5,160,098
sIMPL
OKFHMN_26815: sppA - signal peptide peptidase SppA, at 5,160,214 to 5,162,070
sppA
OKFHMN_26820: ydjA - NAD(P)H nitroreductase, at 5,162,231 to 5,162,782
ydjA
OKFHMN_26825: selD - selenide, water dikinase SelD, at 5,162,899 to 5,163,942
selD
Position (kb) 5160
5161
5162
5163 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5159.237 kb on + strand at 5159.265 kb on + strand at 5159.295 kb on + strand at 5159.295 kb on + strand at 5159.526 kb on - strand, within sIMPL at 5160.078 kb on + strand at 5160.742 kb on - strand, within sppA at 5160.778 kb on - strand, within sppA at 5160.812 kb on + strand, within sppA at 5161.248 kb on - strand, within sppA at 5161.489 kb on - strand, within sppA at 5161.592 kb on + strand, within sppA at 5161.593 kb on - strand, within sppA at 5161.978 kb on - strand at 5161.990 kb on - strand at 5162.073 kb on - strand at 5162.205 kb on + strand at 5162.224 kb on + strand at 5162.347 kb on + strand, within ydjA at 5162.348 kb on - strand, within ydjA at 5162.470 kb on + strand, within ydjA at 5162.530 kb on + strand, within ydjA at 5162.775 kb on - strand at 5162.930 kb on - strand at 5163.034 kb on - strand, within selD
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 5,159,237 + +1.0 5,159,265 + -0.0 5,159,295 + +0.2 5,159,295 + -0.6 5,159,526 - sIMPL OKFHMN_26810 0.19 -0.6 5,160,078 + -0.4 5,160,742 - sppA OKFHMN_26815 0.28 -0.0 5,160,778 - sppA OKFHMN_26815 0.30 +0.4 5,160,812 + sppA OKFHMN_26815 0.32 +0.3 5,161,248 - sppA OKFHMN_26815 0.56 +0.5 5,161,489 - sppA OKFHMN_26815 0.69 +2.5 5,161,592 + sppA OKFHMN_26815 0.74 +0.6 5,161,593 - sppA OKFHMN_26815 0.74 +0.5 5,161,978 - +0.4 5,161,990 - -2.2 5,162,073 - -2.3 5,162,205 + -0.2 5,162,224 + -1.8 5,162,347 + ydjA OKFHMN_26820 0.21 -1.0 5,162,348 - ydjA OKFHMN_26820 0.21 +0.0 5,162,470 + ydjA OKFHMN_26820 0.43 -0.3 5,162,530 + ydjA OKFHMN_26820 0.54 -1.6 5,162,775 - +0.5 5,162,930 - -1.5 5,163,034 - selD OKFHMN_26825 0.13 +0.2
Or see this region's nucleotide sequence