Strain Fitness in Escherichia coli ECRC100 around OKFHMN_26350

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntedd and kdgA are separated by 36 nucleotideskdgA and purT are separated by 55 nucleotidespurT and yebG are separated by 133 nucleotidesyebG and yebF are separated by 66 nucleotidesyebF and OKFHMN_26365 overlap by 87 nucleotides OKFHMN_26340: edd - phosphogluconate dehydratase, at 5,073,984 to 5,075,795 edd OKFHMN_26345: kdgA - bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase, at 5,075,832 to 5,076,473 kdgA OKFHMN_26350: purT - formate-dependent phosphoribosylglycinamide formyltransferase, at 5,076,529 to 5,077,707 purT OKFHMN_26355: yebG - DNA damage-inducible protein YebG, at 5,077,841 to 5,078,131 yebG OKFHMN_26360: yebF - Protein YebF, at 5,078,198 to 5,078,554 yebF OKFHMN_26365: OKFHMN_26365 - hypothetical protein, at 5,078,468 to 5,078,674 _26365 Position (kb) 5076 5077 5078Strain fitness (log2 ratio) -2 -1 0 1at 5075.558 kb on - strand, within eddat 5075.794 kb on - strandat 5076.155 kb on + strand, within kdgAat 5076.156 kb on - strand, within kdgAat 5076.211 kb on - strand, within kdgAat 5076.320 kb on + strand, within kdgAat 5076.336 kb on + strand, within kdgAat 5076.337 kb on - strand, within kdgAat 5076.454 kb on + strandat 5077.132 kb on + strand, within purTat 5077.476 kb on - strand, within purTat 5077.528 kb on - strand, within purTat 5077.531 kb on - strand, within purTat 5077.655 kb on - strandat 5077.658 kb on - strandat 5077.725 kb on + strandat 5077.859 kb on + strandat 5077.859 kb on + strandat 5077.860 kb on - strandat 5077.863 kb on + strandat 5077.863 kb on + strandat 5077.939 kb on + strand, within yebGat 5078.016 kb on + strand, within yebGat 5078.443 kb on + strand, within yebFat 5078.475 kb on + strandat 5078.501 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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5,075,558 - edd OKFHMN_26340 0.87 +0.3
5,075,794 - -0.6
5,076,155 + kdgA OKFHMN_26345 0.50 +1.3
5,076,156 - kdgA OKFHMN_26345 0.50 -1.5
5,076,211 - kdgA OKFHMN_26345 0.59 +1.0
5,076,320 + kdgA OKFHMN_26345 0.76 -0.5
5,076,336 + kdgA OKFHMN_26345 0.79 -1.9
5,076,337 - kdgA OKFHMN_26345 0.79 -1.3
5,076,454 + -1.0
5,077,132 + purT OKFHMN_26350 0.51 -0.3
5,077,476 - purT OKFHMN_26350 0.80 +0.5
5,077,528 - purT OKFHMN_26350 0.85 +0.7
5,077,531 - purT OKFHMN_26350 0.85 -0.2
5,077,655 - -0.9
5,077,658 - +0.1
5,077,725 + -0.9
5,077,859 + +1.2
5,077,859 + -0.6
5,077,860 - +0.4
5,077,863 + -0.6
5,077,863 + -0.2
5,077,939 + yebG OKFHMN_26355 0.34 -0.4
5,078,016 + yebG OKFHMN_26355 0.60 -0.1
5,078,443 + yebF OKFHMN_26360 0.69 -0.3
5,078,475 + +0.5
5,078,501 + +1.4

Or see this region's nucleotide sequence