Strain Fitness in Escherichia coli ECRC100 around OKFHMN_26250

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntaspS and nudB are separated by 60 nucleotidesnudB and OKFHMN_26255 are separated by 28 nucleotidesOKFHMN_26255 and OKFHMN_26260 overlap by 1 nucleotidesOKFHMN_26260 and ruvC overlap by 1 nucleotides OKFHMN_26245: aspS - aspartate--tRNA ligase, at 5,057,967 to 5,059,739 aspS OKFHMN_26250: nudB - dihydroneopterin triphosphate diphosphatase, at 5,059,800 to 5,060,309 nudB OKFHMN_26255: OKFHMN_26255 - putative transcriptional regulatory protein YebC, at 5,060,338 to 5,061,078 _26255 OKFHMN_26260: OKFHMN_26260 - Crossover junction endodeoxyribonuclease RuvC, at 5,061,078 to 5,061,113 _26260 OKFHMN_26265: ruvC - crossover junction endodeoxyribonuclease RuvC, at 5,061,113 to 5,061,634 ruvC Position (kb) 5059 5060 5061Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 5059.740 kb on + strandat 5059.807 kb on + strandat 5059.807 kb on + strandat 5059.811 kb on + strandat 5059.811 kb on + strandat 5059.815 kb on + strandat 5059.815 kb on + strandat 5059.815 kb on + strandat 5059.865 kb on + strand, within nudBat 5059.872 kb on + strand, within nudBat 5059.894 kb on + strand, within nudBat 5059.905 kb on + strand, within nudBat 5059.916 kb on + strand, within nudBat 5060.026 kb on + strand, within nudBat 5060.071 kb on + strand, within nudBat 5060.116 kb on + strand, within nudBat 5060.293 kb on + strandat 5060.415 kb on + strand, within OKFHMN_26255at 5060.830 kb on + strand, within OKFHMN_26255at 5060.854 kb on + strand, within OKFHMN_26255at 5060.854 kb on + strand, within OKFHMN_26255

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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5,059,740 + -0.5
5,059,807 + -0.4
5,059,807 + -0.5
5,059,811 + +0.3
5,059,811 + -0.2
5,059,815 + +0.2
5,059,815 + -0.8
5,059,815 + +0.6
5,059,865 + nudB OKFHMN_26250 0.13 -4.5
5,059,872 + nudB OKFHMN_26250 0.14 -2.3
5,059,894 + nudB OKFHMN_26250 0.18 -0.9
5,059,905 + nudB OKFHMN_26250 0.21 -1.5
5,059,916 + nudB OKFHMN_26250 0.23 -3.5
5,060,026 + nudB OKFHMN_26250 0.44 -1.4
5,060,071 + nudB OKFHMN_26250 0.53 -2.0
5,060,116 + nudB OKFHMN_26250 0.62 -2.0
5,060,293 + -1.3
5,060,415 + OKFHMN_26255 0.10 -1.4
5,060,830 + OKFHMN_26255 0.66 -0.5
5,060,854 + OKFHMN_26255 0.70 -0.9
5,060,854 + OKFHMN_26255 0.70 +0.9

Or see this region's nucleotide sequence