Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uvrC and pgsA are separated by 56 nucleotides pgsA and Gly_trna are separated by 151 nucleotides Gly_trna and Cys_trna are separated by 53 nucleotides Cys_trna and Leu_trna are separated by 12 nucleotides
OKFHMN_25825: uvrC - excinuclease ABC subunit UvrC, at 4,992,128 to 4,993,960
uvrC
OKFHMN_25830: pgsA - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 4,994,017 to 4,994,565
pgsA
OKFHMN_25835: Gly_trna - tRNA-Gly(gcc), at 4,994,717 to 4,994,792
_trna
OKFHMN_25840: Cys_trna - tRNA-Cys(gca), at 4,994,846 to 4,994,919
_trna
OKFHMN_25845: Leu_trna - tRNA-Leu(taa), at 4,994,932 to 4,995,018
_trna
Position (kb) 4994
4995 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4993.037 kb on + strand, within uvrC at 4993.041 kb on + strand, within uvrC at 4993.333 kb on + strand, within uvrC at 4993.412 kb on - strand, within uvrC at 4993.412 kb on - strand, within uvrC at 4993.498 kb on + strand, within uvrC at 4993.498 kb on + strand, within uvrC at 4993.851 kb on - strand at 4994.683 kb on + strand at 4995.461 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 4,993,037 + uvrC OKFHMN_25825 0.50 -0.0 4,993,041 + uvrC OKFHMN_25825 0.50 -3.3 4,993,333 + uvrC OKFHMN_25825 0.66 +0.3 4,993,412 - uvrC OKFHMN_25825 0.70 +0.2 4,993,412 - uvrC OKFHMN_25825 0.70 +0.1 4,993,498 + uvrC OKFHMN_25825 0.75 +1.1 4,993,498 + uvrC OKFHMN_25825 0.75 -1.6 4,993,851 - +0.1 4,994,683 + -1.5 4,995,461 + +1.9
Or see this region's nucleotide sequence