Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yedJ and dcm are separated by 66 nucleotides dcm and vsr overlap by 20 nucleotides vsr and yedA overlap by 12 nucleotides
OKFHMN_25595: yedJ - phosphohydrolase, at 4,953,504 to 4,954,199
yedJ
OKFHMN_25600: dcm - DNA-cytosine methyltransferase, at 4,954,266 to 4,955,684
dcm
OKFHMN_25605: vsr - VSPR family DNA mismatch endonuclease, at 4,955,665 to 4,956,135
vsr
OKFHMN_25610: yedA - drug/metabolite exporter YedA, at 4,956,124 to 4,957,044
yedA
Position (kb)
4954
4955
4956 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4953.277 kb on + strand at 4953.472 kb on + strand at 4953.511 kb on + strand at 4953.512 kb on - strand at 4954.045 kb on + strand, within yedJ at 4954.174 kb on + strand at 4954.225 kb on + strand at 4954.348 kb on - strand at 4954.348 kb on - strand at 4954.518 kb on + strand, within dcm at 4954.745 kb on + strand, within dcm at 4955.256 kb on + strand, within dcm at 4955.257 kb on - strand, within dcm at 4955.850 kb on + strand, within vsr at 4955.850 kb on + strand, within vsr at 4955.885 kb on - strand, within vsr at 4955.933 kb on + strand, within vsr at 4955.933 kb on + strand, within vsr at 4956.021 kb on - strand, within vsr at 4956.347 kb on - strand, within yedA at 4956.351 kb on - strand, within yedA at 4956.351 kb on - strand, within yedA at 4956.354 kb on + strand, within yedA at 4956.354 kb on + strand, within yedA at 4956.355 kb on - strand, within yedA at 4956.355 kb on - strand, within yedA at 4956.355 kb on - strand, within yedA at 4956.355 kb on - strand, within yedA at 4956.356 kb on + strand, within yedA at 4956.469 kb on + strand, within yedA at 4956.517 kb on + strand, within yedA
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 4,953,277 + -0.8 4,953,472 + -0.9 4,953,511 + +0.2 4,953,512 - -0.2 4,954,045 + yedJ OKFHMN_25595 0.78 -0.4 4,954,174 + +0.8 4,954,225 + -0.2 4,954,348 - +0.9 4,954,348 - -1.1 4,954,518 + dcm OKFHMN_25600 0.18 +0.6 4,954,745 + dcm OKFHMN_25600 0.34 -0.3 4,955,256 + dcm OKFHMN_25600 0.70 -0.4 4,955,257 - dcm OKFHMN_25600 0.70 -1.0 4,955,850 + vsr OKFHMN_25605 0.39 -0.5 4,955,850 + vsr OKFHMN_25605 0.39 +0.3 4,955,885 - vsr OKFHMN_25605 0.47 +2.0 4,955,933 + vsr OKFHMN_25605 0.57 +0.4 4,955,933 + vsr OKFHMN_25605 0.57 +0.3 4,956,021 - vsr OKFHMN_25605 0.76 +1.7 4,956,347 - yedA OKFHMN_25610 0.24 -2.7 4,956,351 - yedA OKFHMN_25610 0.25 +0.3 4,956,351 - yedA OKFHMN_25610 0.25 +0.1 4,956,354 + yedA OKFHMN_25610 0.25 +0.2 4,956,354 + yedA OKFHMN_25610 0.25 +0.1 4,956,355 - yedA OKFHMN_25610 0.25 +0.1 4,956,355 - yedA OKFHMN_25610 0.25 -0.9 4,956,355 - yedA OKFHMN_25610 0.25 -0.1 4,956,355 - yedA OKFHMN_25610 0.25 -0.8 4,956,356 + yedA OKFHMN_25610 0.25 -0.4 4,956,469 + yedA OKFHMN_25610 0.37 -3.0 4,956,517 + yedA OKFHMN_25610 0.43 +0.1
Or see this region's nucleotide sequence