Strain Fitness in Escherichia coli ECRC100 around OKFHMN_22745

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisQ and hisM overlap by 4 nucleotideshisM and hisP are separated by 7 nucleotideshisP and rpnB are separated by 196 nucleotides OKFHMN_22735: hisQ - histidine ABC transporter permease HisQ, at 4,422,583 to 4,423,269 hisQ OKFHMN_22740: hisM - Histidine transport system permease protein HisM, at 4,423,266 to 4,423,982 hisM OKFHMN_22745: hisP - histidine ABC transporter ATP-binding protein HisP, at 4,423,990 to 4,424,763 hisP OKFHMN_22750: rpnB - recombination-promoting nuclease RpnB, at 4,424,960 to 4,425,850 rpnB Position (kb) 4423 4424 4425Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4423.075 kb on - strand, within hisQat 4423.425 kb on + strand, within hisMat 4423.512 kb on + strand, within hisMat 4423.513 kb on - strand, within hisMat 4423.513 kb on - strand, within hisMat 4423.529 kb on + strand, within hisMat 4423.737 kb on - strand, within hisMat 4423.784 kb on + strand, within hisMat 4423.855 kb on - strand, within hisMat 4423.908 kb on + strand, within hisMat 4423.909 kb on - strand, within hisMat 4423.909 kb on - strand, within hisMat 4424.088 kb on - strand, within hisPat 4424.088 kb on - strand, within hisPat 4424.090 kb on + strand, within hisPat 4424.124 kb on + strand, within hisPat 4424.142 kb on + strand, within hisPat 4424.308 kb on - strand, within hisPat 4424.646 kb on + strand, within hisPat 4424.678 kb on + strand, within hisPat 4424.746 kb on - strandat 4424.761 kb on + strandat 4424.762 kb on - strandat 4425.405 kb on + strand, within rpnBat 4425.460 kb on + strand, within rpnBat 4425.531 kb on + strand, within rpnBat 4425.559 kb on + strand, within rpnBat 4425.585 kb on + strand, within rpnBat 4425.598 kb on + strand, within rpnBat 4425.598 kb on + strand, within rpnBat 4425.598 kb on + strand, within rpnBat 4425.603 kb on - strand, within rpnBat 4425.607 kb on - strand, within rpnBat 4425.607 kb on - strand, within rpnBat 4425.610 kb on - strand, within rpnBat 4425.655 kb on - strand, within rpnBat 4425.709 kb on - strand, within rpnB

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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4,423,075 - hisQ OKFHMN_22735 0.72 +0.1
4,423,425 + hisM OKFHMN_22740 0.22 +0.3
4,423,512 + hisM OKFHMN_22740 0.34 +0.1
4,423,513 - hisM OKFHMN_22740 0.34 -1.3
4,423,513 - hisM OKFHMN_22740 0.34 -0.1
4,423,529 + hisM OKFHMN_22740 0.37 -0.3
4,423,737 - hisM OKFHMN_22740 0.66 -1.6
4,423,784 + hisM OKFHMN_22740 0.72 +1.3
4,423,855 - hisM OKFHMN_22740 0.82 -0.4
4,423,908 + hisM OKFHMN_22740 0.90 +0.2
4,423,909 - hisM OKFHMN_22740 0.90 -0.1
4,423,909 - hisM OKFHMN_22740 0.90 -0.1
4,424,088 - hisP OKFHMN_22745 0.13 -2.0
4,424,088 - hisP OKFHMN_22745 0.13 -1.0
4,424,090 + hisP OKFHMN_22745 0.13 +0.3
4,424,124 + hisP OKFHMN_22745 0.17 -0.6
4,424,142 + hisP OKFHMN_22745 0.20 -0.1
4,424,308 - hisP OKFHMN_22745 0.41 -3.6
4,424,646 + hisP OKFHMN_22745 0.85 -0.3
4,424,678 + hisP OKFHMN_22745 0.89 +1.6
4,424,746 - +0.1
4,424,761 + +1.1
4,424,762 - +2.1
4,425,405 + rpnB OKFHMN_22750 0.50 -2.2
4,425,460 + rpnB OKFHMN_22750 0.56 +0.4
4,425,531 + rpnB OKFHMN_22750 0.64 -0.7
4,425,559 + rpnB OKFHMN_22750 0.67 +0.6
4,425,585 + rpnB OKFHMN_22750 0.70 -0.3
4,425,598 + rpnB OKFHMN_22750 0.72 +1.0
4,425,598 + rpnB OKFHMN_22750 0.72 -1.6
4,425,598 + rpnB OKFHMN_22750 0.72 -2.1
4,425,603 - rpnB OKFHMN_22750 0.72 +0.4
4,425,607 - rpnB OKFHMN_22750 0.73 +0.4
4,425,607 - rpnB OKFHMN_22750 0.73 -0.1
4,425,610 - rpnB OKFHMN_22750 0.73 -0.3
4,425,655 - rpnB OKFHMN_22750 0.78 +0.0
4,425,709 - rpnB OKFHMN_22750 0.84 +0.2

Or see this region's nucleotide sequence