Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyfF and hyfE are separated by 19 nucleotides hyfE and hyfD are separated by 11 nucleotides hyfD and hyfC are separated by 16 nucleotides hyfC and hyfB are separated by 10 nucleotides
OKFHMN_21875: hyfF - hydrogenase 4 subunit F, at 4,249,987 to 4,251,552
hyfF
OKFHMN_21880: hyfE - hydrogenase 4 membrane subunit, at 4,251,572 to 4,252,222
hyfE
OKFHMN_21885: hyfD - hydrogenase 4 subunit HyfD, at 4,252,234 to 4,253,673
hyfD
OKFHMN_21890: hyfC - hydrogenase 4 subunit HyfC, at 4,253,690 to 4,254,637
hyfC
OKFHMN_21895: hyfB - hydrogenase 4 subunit B, at 4,254,648 to 4,256,666
hyfB
Position (kb) 4252
4253
4254 Strain fitness (log2 ratio) -2
-1
0
1 at 4251.355 kb on - strand, within hyfF at 4251.451 kb on - strand at 4251.451 kb on - strand at 4251.454 kb on + strand at 4251.455 kb on - strand at 4251.455 kb on - strand at 4251.561 kb on - strand at 4251.641 kb on + strand, within hyfE at 4251.716 kb on + strand, within hyfE at 4251.870 kb on - strand, within hyfE at 4251.991 kb on - strand, within hyfE at 4251.991 kb on - strand, within hyfE at 4251.991 kb on - strand, within hyfE at 4251.991 kb on - strand, within hyfE at 4252.027 kb on + strand, within hyfE at 4252.199 kb on - strand at 4252.202 kb on + strand at 4252.211 kb on - strand at 4252.235 kb on + strand at 4252.289 kb on - strand at 4252.448 kb on + strand, within hyfD at 4252.579 kb on + strand, within hyfD at 4252.579 kb on + strand, within hyfD at 4252.579 kb on + strand, within hyfD at 4252.637 kb on - strand, within hyfD at 4252.680 kb on - strand, within hyfD at 4253.054 kb on + strand, within hyfD at 4253.055 kb on - strand, within hyfD at 4253.136 kb on - strand, within hyfD at 4253.136 kb on - strand, within hyfD at 4253.163 kb on + strand, within hyfD at 4253.163 kb on + strand, within hyfD at 4253.164 kb on - strand, within hyfD at 4253.176 kb on + strand, within hyfD at 4253.208 kb on - strand, within hyfD at 4253.211 kb on - strand, within hyfD at 4253.285 kb on - strand, within hyfD at 4253.647 kb on + strand at 4253.840 kb on - strand, within hyfC at 4254.006 kb on - strand, within hyfC at 4254.089 kb on + strand, within hyfC at 4254.127 kb on + strand, within hyfC at 4254.309 kb on + strand, within hyfC at 4254.310 kb on - strand, within hyfC at 4254.310 kb on - strand, within hyfC at 4254.310 kb on - strand, within hyfC at 4254.358 kb on + strand, within hyfC at 4254.358 kb on + strand, within hyfC at 4254.440 kb on + strand, within hyfC at 4254.442 kb on + strand, within hyfC at 4254.443 kb on - strand, within hyfC at 4254.523 kb on + strand, within hyfC at 4254.533 kb on + strand, within hyfC at 4254.612 kb on + strand at 4254.660 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 4,251,355 - hyfF OKFHMN_21875 0.87 +0.0 4,251,451 - +0.4 4,251,451 - +0.0 4,251,454 + -0.1 4,251,455 - +0.9 4,251,455 - +0.1 4,251,561 - +0.3 4,251,641 + hyfE OKFHMN_21880 0.11 +0.1 4,251,716 + hyfE OKFHMN_21880 0.22 +0.7 4,251,870 - hyfE OKFHMN_21880 0.46 +0.3 4,251,991 - hyfE OKFHMN_21880 0.64 -1.1 4,251,991 - hyfE OKFHMN_21880 0.64 +0.3 4,251,991 - hyfE OKFHMN_21880 0.64 -0.1 4,251,991 - hyfE OKFHMN_21880 0.64 +0.5 4,252,027 + hyfE OKFHMN_21880 0.70 +1.2 4,252,199 - -1.0 4,252,202 + +0.3 4,252,211 - -0.0 4,252,235 + -0.3 4,252,289 - -2.3 4,252,448 + hyfD OKFHMN_21885 0.15 +0.3 4,252,579 + hyfD OKFHMN_21885 0.24 -0.6 4,252,579 + hyfD OKFHMN_21885 0.24 -0.9 4,252,579 + hyfD OKFHMN_21885 0.24 -0.5 4,252,637 - hyfD OKFHMN_21885 0.28 -1.9 4,252,680 - hyfD OKFHMN_21885 0.31 +0.6 4,253,054 + hyfD OKFHMN_21885 0.57 -0.4 4,253,055 - hyfD OKFHMN_21885 0.57 +0.1 4,253,136 - hyfD OKFHMN_21885 0.63 +0.4 4,253,136 - hyfD OKFHMN_21885 0.63 +0.8 4,253,163 + hyfD OKFHMN_21885 0.65 +1.3 4,253,163 + hyfD OKFHMN_21885 0.65 -0.4 4,253,164 - hyfD OKFHMN_21885 0.65 -0.0 4,253,176 + hyfD OKFHMN_21885 0.65 -0.5 4,253,208 - hyfD OKFHMN_21885 0.68 -0.2 4,253,211 - hyfD OKFHMN_21885 0.68 -1.4 4,253,285 - hyfD OKFHMN_21885 0.73 -0.1 4,253,647 + +0.9 4,253,840 - hyfC OKFHMN_21890 0.16 -0.4 4,254,006 - hyfC OKFHMN_21890 0.33 -0.1 4,254,089 + hyfC OKFHMN_21890 0.42 +1.2 4,254,127 + hyfC OKFHMN_21890 0.46 -1.1 4,254,309 + hyfC OKFHMN_21890 0.65 -1.5 4,254,310 - hyfC OKFHMN_21890 0.65 -0.7 4,254,310 - hyfC OKFHMN_21890 0.65 +0.3 4,254,310 - hyfC OKFHMN_21890 0.65 +0.1 4,254,358 + hyfC OKFHMN_21890 0.70 -0.3 4,254,358 + hyfC OKFHMN_21890 0.70 +0.9 4,254,440 + hyfC OKFHMN_21890 0.79 -1.1 4,254,442 + hyfC OKFHMN_21890 0.79 +0.1 4,254,443 - hyfC OKFHMN_21890 0.79 -1.1 4,254,523 + hyfC OKFHMN_21890 0.88 +0.5 4,254,533 + hyfC OKFHMN_21890 0.89 -0.2 4,254,612 + +1.2 4,254,660 - +0.4
Or see this region's nucleotide sequence