Strain Fitness in Escherichia coli ECRC100 around OKFHMN_20380

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcysI and cysH are separated by 73 nucleotidescysH and hokG are separated by 263 nucleotideshokG and cas3 are separated by 191 nucleotides OKFHMN_20375: cysI - assimilatory sulfite reductase (NADPH) hemoprotein subunit, at 3,966,172 to 3,967,884 cysI OKFHMN_20380: cysH - phosphoadenosine phosphosulfate reductase, at 3,967,958 to 3,968,692 cysH OKFHMN_20390: hokG - Putative protein HokG, at 3,968,956 to 3,969,108 hokG OKFHMN_20395: cas3 - CRISPR-associated helicase/endonuclease Cas3, at 3,969,300 to 3,971,999 cas3 Position (kb) 3967 3968 3969Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3966.997 kb on + strand, within cysIat 3966.997 kb on + strand, within cysIat 3967.096 kb on + strand, within cysIat 3967.097 kb on - strand, within cysIat 3967.128 kb on + strand, within cysIat 3967.207 kb on - strand, within cysIat 3967.234 kb on - strand, within cysIat 3967.584 kb on + strand, within cysIat 3967.723 kb on + strandat 3967.724 kb on - strandat 3967.958 kb on - strandat 3968.029 kb on + strandat 3968.100 kb on - strand, within cysHat 3968.236 kb on - strand, within cysHat 3968.268 kb on - strand, within cysHat 3968.349 kb on - strand, within cysHat 3968.526 kb on + strand, within cysHat 3968.528 kb on + strand, within cysHat 3968.558 kb on + strand, within cysHat 3968.605 kb on + strand, within cysHat 3968.803 kb on + strandat 3968.804 kb on - strandat 3968.804 kb on - strandat 3968.880 kb on + strandat 3968.928 kb on + strandat 3968.988 kb on - strand, within hokGat 3968.988 kb on - strand, within hokGat 3968.988 kb on - strand, within hokGat 3968.991 kb on - strand, within hokGat 3969.000 kb on + strand, within hokGat 3969.000 kb on + strand, within hokGat 3969.000 kb on + strand, within hokGat 3969.038 kb on + strand, within hokGat 3969.338 kb on + strandat 3969.594 kb on + strand, within cas3at 3969.595 kb on - strand, within cas3

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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3,966,997 + cysI OKFHMN_20375 0.48 -3.1
3,966,997 + cysI OKFHMN_20375 0.48 -3.8
3,967,096 + cysI OKFHMN_20375 0.54 -1.7
3,967,097 - cysI OKFHMN_20375 0.54 -2.0
3,967,128 + cysI OKFHMN_20375 0.56 -2.2
3,967,207 - cysI OKFHMN_20375 0.60 -2.0
3,967,234 - cysI OKFHMN_20375 0.62 -2.2
3,967,584 + cysI OKFHMN_20375 0.82 -3.5
3,967,723 + -2.4
3,967,724 - -3.6
3,967,958 - -4.8
3,968,029 + -5.2
3,968,100 - cysH OKFHMN_20380 0.19 -2.5
3,968,236 - cysH OKFHMN_20380 0.38 -1.7
3,968,268 - cysH OKFHMN_20380 0.42 -3.8
3,968,349 - cysH OKFHMN_20380 0.53 -1.7
3,968,526 + cysH OKFHMN_20380 0.77 +0.3
3,968,528 + cysH OKFHMN_20380 0.78 -5.2
3,968,558 + cysH OKFHMN_20380 0.82 -4.7
3,968,605 + cysH OKFHMN_20380 0.88 -1.6
3,968,803 + +0.7
3,968,804 - +1.4
3,968,804 - -0.1
3,968,880 + +0.0
3,968,928 + +0.1
3,968,988 - hokG OKFHMN_20390 0.21 -0.2
3,968,988 - hokG OKFHMN_20390 0.21 +0.2
3,968,988 - hokG OKFHMN_20390 0.21 -0.5
3,968,991 - hokG OKFHMN_20390 0.23 +0.1
3,969,000 + hokG OKFHMN_20390 0.29 +0.2
3,969,000 + hokG OKFHMN_20390 0.29 -0.2
3,969,000 + hokG OKFHMN_20390 0.29 +0.3
3,969,038 + hokG OKFHMN_20390 0.54 -0.7
3,969,338 + -0.7
3,969,594 + cas3 OKFHMN_20395 0.11 -1.7
3,969,595 - cas3 OKFHMN_20395 0.11 -1.2

Or see this region's nucleotide sequence