Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nikE and nikD overlap by 4 nucleotides nikD and nikC overlap by 1 nucleotides nikC and nikB overlap by 4 nucleotides nikB and nikA overlap by 1 nucleotides
OKFHMN_16530: nikE - nickel import ATP-binding protein NikE, at 3,221,897 to 3,222,703
nikE
OKFHMN_16535: nikD - nickel import ATP-binding protein NikD, at 3,222,700 to 3,223,464
nikD
OKFHMN_16540: nikC - nickel ABC transporter permease subunit NikC, at 3,223,464 to 3,224,297
nikC
OKFHMN_16545: nikB - nickel ABC transporter permease subunit NikB, at 3,224,294 to 3,225,238
nikB
OKFHMN_16550: nikA - nickel ABC transporter substrate-binding protein, at 3,225,238 to 3,226,812
nikA
Position (kb)
3223
3224
3225 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3222.497 kb on - strand, within nikE at 3222.521 kb on + strand, within nikE at 3222.666 kb on + strand at 3222.692 kb on - strand at 3222.692 kb on - strand at 3222.695 kb on + strand at 3222.695 kb on + strand at 3222.695 kb on + strand at 3222.696 kb on - strand at 3222.723 kb on - strand at 3222.865 kb on - strand, within nikD at 3222.907 kb on - strand, within nikD at 3222.981 kb on + strand, within nikD at 3222.981 kb on + strand, within nikD at 3223.099 kb on + strand, within nikD at 3223.099 kb on + strand, within nikD at 3223.099 kb on + strand, within nikD at 3223.117 kb on - strand, within nikD at 3223.440 kb on + strand at 3223.440 kb on + strand at 3223.440 kb on + strand at 3223.441 kb on - strand at 3223.441 kb on - strand at 3223.444 kb on + strand at 3223.444 kb on + strand at 3223.445 kb on - strand at 3223.547 kb on + strand at 3223.595 kb on + strand, within nikC at 3223.595 kb on + strand, within nikC at 3223.596 kb on - strand, within nikC at 3223.692 kb on - strand, within nikC at 3223.884 kb on - strand, within nikC at 3223.887 kb on + strand, within nikC at 3223.888 kb on - strand, within nikC at 3223.888 kb on - strand, within nikC at 3223.923 kb on - strand, within nikC at 3224.198 kb on - strand, within nikC at 3224.365 kb on + strand at 3224.366 kb on - strand at 3224.399 kb on - strand, within nikB at 3224.399 kb on - strand, within nikB at 3224.683 kb on + strand, within nikB at 3224.730 kb on + strand, within nikB at 3224.907 kb on - strand, within nikB at 3224.910 kb on - strand, within nikB at 3225.045 kb on + strand, within nikB at 3225.045 kb on + strand, within nikB at 3225.224 kb on + strand at 3225.225 kb on - strand at 3225.229 kb on - strand at 3225.240 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 3,222,497 - nikE OKFHMN_16530 0.74 +0.0 3,222,521 + nikE OKFHMN_16530 0.77 -0.3 3,222,666 + +0.1 3,222,692 - -0.8 3,222,692 - +0.7 3,222,695 + +0.7 3,222,695 + +0.7 3,222,695 + -0.9 3,222,696 - +0.9 3,222,723 - -0.1 3,222,865 - nikD OKFHMN_16535 0.22 +0.4 3,222,907 - nikD OKFHMN_16535 0.27 +0.3 3,222,981 + nikD OKFHMN_16535 0.37 -0.5 3,222,981 + nikD OKFHMN_16535 0.37 -0.3 3,223,099 + nikD OKFHMN_16535 0.52 +0.3 3,223,099 + nikD OKFHMN_16535 0.52 +0.1 3,223,099 + nikD OKFHMN_16535 0.52 +0.0 3,223,117 - nikD OKFHMN_16535 0.55 -1.3 3,223,440 + +0.1 3,223,440 + -2.1 3,223,440 + +0.3 3,223,441 - -0.1 3,223,441 - -0.5 3,223,444 + +0.4 3,223,444 + -0.6 3,223,445 - -0.0 3,223,547 + +1.7 3,223,595 + nikC OKFHMN_16540 0.16 +0.2 3,223,595 + nikC OKFHMN_16540 0.16 -1.2 3,223,596 - nikC OKFHMN_16540 0.16 -0.2 3,223,692 - nikC OKFHMN_16540 0.27 +0.3 3,223,884 - nikC OKFHMN_16540 0.50 +0.8 3,223,887 + nikC OKFHMN_16540 0.51 +2.3 3,223,888 - nikC OKFHMN_16540 0.51 +0.9 3,223,888 - nikC OKFHMN_16540 0.51 +0.7 3,223,923 - nikC OKFHMN_16540 0.55 +0.3 3,224,198 - nikC OKFHMN_16540 0.88 +0.6 3,224,365 + +0.5 3,224,366 - -0.6 3,224,399 - nikB OKFHMN_16545 0.11 +0.1 3,224,399 - nikB OKFHMN_16545 0.11 -0.9 3,224,683 + nikB OKFHMN_16545 0.41 -0.2 3,224,730 + nikB OKFHMN_16545 0.46 +0.3 3,224,907 - nikB OKFHMN_16545 0.65 -0.7 3,224,910 - nikB OKFHMN_16545 0.65 -0.2 3,225,045 + nikB OKFHMN_16545 0.79 +0.4 3,225,045 + nikB OKFHMN_16545 0.79 +0.4 3,225,224 + +0.1 3,225,225 - -0.4 3,225,229 - +1.5 3,225,240 - -0.1
Or see this region's nucleotide sequence